Q21178 (NAS17_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc metalloproteinase nas-17
EC=3.4.24.21

Alternative name(s):
Nematode astacin 17

Gene names

Name:	nas-17
ORF Names:	K03B8.2

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable metalloprotease By similarity.
Catalytic activity	Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the peptidase M12A family. Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	EGF-like domain Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 429	408	Zinc metalloproteinase nas-17		PRO_0000028921
Regions
	Domain	245 – 284	40	EGF-like
Sites
	Active site	153	1	By similarity
	Metal binding	152	1	Zinc; catalytic By similarity
	Metal binding	156	1	Zinc; catalytic By similarity
	Metal binding	162	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	54	1	N-linked (GlcNAc...) Potential
	Disulfide bond	250 ↔ 258		By similarity
	Disulfide bond	252 ↔ 272		By similarity
	Disulfide bond	274 ↔ 283		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q21178 [UniParc]. Last modified January 4, 2005. Version 2. Checksum: D3C603863FD4C9BF	FASTA	429	48,563
	10 20 30 40 50 60 MFLRPSTLLL TLFLALVAGS AIRKDVDEFD SNKGKDGIVD GDIMLTEAQL RILNGTAKRS 70 80 90 100 110 120 KRQITKIWKK WPDAKVFYYY ENEFTSLKRE LMSYAMAHIS SNTCVKFQES NSATNRIRFT 130 140 150 160 170 180 NTGGCASYIG MNGGEQTLWF GDGCLIFGTA VHEIMHSLGL FHTHSRFDRD NFLSVSYKDV 190 200 210 220 230 240 PENMVGNLEK ETEQTTYNAV PFEYGSTMLY RYNTFGEGTL VSKNEDYQKT MGLRRVSFYD 250 260 270 280 290 300 LVNINVRYSC GCAKSLTCEN GGYTNPSNCA TCVCPTGFAG TLCNEAPSNT IKLTAESYWK 310 320 330 340 350 360 GYWVNFGYST SIQTTNYYLA YLWITAPADK TIEVKIMDLS GFTCSYGCNY NGVEVKYMGD 370 380 390 400 410 420 PRITNPLRCC AQDTEYLNQV ISSKQNPTPI VMQQRYGSSK LTIHYRYVDT PLSSNKKSTN GYDNYQYYV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"The astacin protein family in Caenorhabditis elegans." Moehrlen F., Hutter H., Zwilling R. Eur. J. Biochem. 270:4909-4920(2003) [PubMed: 14653817] [Abstract] Cited for: IDENTIFICATION, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z74039 Genomic DNA. Translation: CAA98501.1.
PIR	T23263.
RefSeq	NP_505892.1. NM_073491.1.
UniGene	Cel.32978.
3D structure databases
ProteinModelPortal	Q21178.
SMR	Q21178. Positions 34-287, 305-410.
ModBase	Search...
Protein family/group databases
MEROPS	M12.A31.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	186923.
KEGG	cel:K03B8.2.
NMPDR	fig|6239.3.peg.19718.
UCSC	K03B8.2. c. elegans.
Organism-specific databases
CTD	186923.
WormBase	K03B8.2; CE06076; WBGene00003536; nas-17.
Phylogenomic databases
eggNOG	KOG3714.
GeneTree	EMGT00050000002037.
HOGENOM	HBG385598.
InParanoid	Q21178.
Gene expression databases
ArrayExpress	Q21178.
Family and domain databases
InterPro	IPR006210. EGF-like. IPR024079. MetalloPept_cat_dom. IPR001506. Peptidase_M12A. IPR017050. Peptidase_M12A_nem. IPR006026. Peptidase_Metallo. [Graphical view]
Gene3D	G3DSA:3.40.390.10. G3DSA:3.40.390.10. 1 hit.
KO	K08076.
Pfam	PF01400. Astacin. 1 hit. [Graphical view]
PIRSF	PIRSF036365. Astacin_nematoda. 1 hit.
PRINTS	PR00480. ASTACIN.
SMART	SM00181. EGF. 1 hit. SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAS17_CAEEL

Accession

Primary (citable) accession number: Q21178

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	January 4, 2005
Last modified:	November 16, 2011
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project