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Protein

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase

Gene

pfkb-1.2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Synthesis and degradation of fructose 2,6-bisphosphate.By similarity

Catalytic activityi

Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691Fructose 6-phosphateBy similarity
Binding sitei98 – 981Fructose 6-phosphateBy similarity
Active sitei124 – 1241Sequence analysis
Binding sitei126 – 1261Fructose 6-phosphateBy similarity
Binding sitei132 – 1321Fructose 6-phosphateBy similarity
Active sitei154 – 1541Sequence analysis
Binding sitei168 – 1681Fructose 6-phosphateBy similarity
Binding sitei190 – 1901Fructose 6-phosphateBy similarity
Binding sitei194 – 1941Fructose 6-phosphateBy similarity
Binding sitei252 – 2521Fructose 2,6-bisphosphateBy similarity
Sitei252 – 2521Transition state stabilizerBy similarity
Active sitei253 – 2531Tele-phosphohistidine intermediateBy similarity
Binding sitei259 – 2591Fructose 2,6-bisphosphateBy similarity
Sitei259 – 2591Transition state stabilizerBy similarity
Binding sitei265 – 2651Fructose 2,6-bisphosphate; via amide nitrogenBy similarity
Active sitei324 – 3241Proton donor/acceptorBy similarity
Binding sitei335 – 3351Fructose 2,6-bisphosphateBy similarity
Binding sitei349 – 3491Fructose 2,6-bisphosphateBy similarity
Binding sitei353 – 3531Fructose 2,6-bisphosphateBy similarity
Binding sitei364 – 3641Fructose 2,6-bisphosphateBy similarity
Sitei389 – 3891Transition state stabilizerBy similarity
Binding sitei390 – 3901Fructose 2,6-bisphosphateBy similarity
Binding sitei394 – 3941Fructose 2,6-bisphosphateBy similarity
Binding sitei426 – 4261ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 449ATPBy similarity
Nucleotide bindingi163 – 1686ATPBy similarity
Nucleotide bindingi346 – 3494ATPBy similarity
Nucleotide bindingi390 – 3945ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-70171. Glycolysis.
R-CEL-70263. Gluconeogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phosphofructo-2-kinase/fructose-2,6-bisphosphataseImported
Short name:
6PF-2-K/Fru-2,6-P2ase
Short name:
PFK/FBPase
Including the following 2 domains:
Fructose-2,6-bisphosphatase (EC:3.1.3.46)
Gene namesi
Name:pfkb-1.2Imported
ORF Names:K02B2.1Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiK02B2.1; CE30082; WBGene00019295; pfkb-1.2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4574576-phosphofructo-2-kinase/fructose-2,6-bisphosphataseCuratedPRO_0000179974Add
BLAST

Proteomic databases

EPDiQ21122.
PaxDbiQ21122.
PRIDEiQ21122.

Expressioni

Gene expression databases

BgeeiWBGene00019295.

Interactioni

Protein-protein interaction databases

STRINGi6239.K02B2.1.

Structurei

3D structure databases

ProteinModelPortaliQ21122.
SMRiQ21122. Positions 29-439.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2442446-phosphofructo-2-kinaseAdd
BLAST
Regioni245 – 457213Fructose-2,6-bisphosphataseAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated

Phylogenomic databases

eggNOGiKOG0234. Eukaryota.
COG0406. LUCA.
GeneTreeiENSGT00390000018751.
HOGENOMiHOG000181112.
InParanoidiQ21122.
OMAiADEMPYL.
OrthoDBiEOG091G0A43.
PhylomeDBiQ21122.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR027417. P-loop_NTPase.
IPR001345. PG/BPGM_mutase_AS.
[Graphical view]
PANTHERiPTHR10606. PTHR10606. 1 hit.
PfamiPF01591. 6PF2K. 1 hit.
PF00300. His_Phos_1. 1 hit.
[Graphical view]
PRINTSiPR00991. 6PFRUCTKNASE.
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53254. SSF53254. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q21122-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIPPGLETT KRKVAHSDEH GFSDQVRVPN VIVMVGLPAR GKTYISKKLC
60 70 80 90 100
RYLKWTGFTT KVFNVGEYRR SDANAADAIH GANASFFSPN NADALKVRAE
110 120 130 140 150
SARRAMEDMA DYLNSGTGGV AIFDATNTTK DRRRIIIDFC KKQRLRCFFI
160 170 180 190 200
ESVCDDPAII DCNVTDVKVN SPDYKGLMTA EQAKEDFMNR IENYKKQYEP
210 220 230 240 250
LDESEDESLS FIKVINAGRS FKVHQVRGHV QSRVVYFLMN IHLLPRSIYL
260 270 280 290 300
TRHGQSEYNA MGRLGGDSPL TEDGQKYASA LADFFEEEEV PGLRVWCSQK
310 320 330 340 350
VRAAQTAQHL KPDFHTEYWK ALDELDAGIC EGLTYEDILQ RYPKQADDRA
360 370 380 390 400
TDKYHYRYPS GESYEDVVSR LEPVIMELER QANVLVVSHQ AVLRCVLAYF
410 420 430 440 450
YDRPLSELPY IDIPLHSLVK LTPRAYHCDS TIYALDLESG EWTETSDQLP

LCDSPRD
Length:457
Mass (Da):52,091
Last modified:June 6, 2002 - v2
Checksum:iE04A9A892378E413
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080181 Genomic DNA. Translation: CCD61801.1.
RefSeqiNP_500893.2. NM_068492.5.
UniGeneiCel.7619.

Genome annotation databases

EnsemblMetazoaiK02B2.1; K02B2.1; WBGene00019295.
GeneIDi177363.
KEGGicel:CELE_K02B2.1.
UCSCiK02B2.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080181 Genomic DNA. Translation: CCD61801.1.
RefSeqiNP_500893.2. NM_068492.5.
UniGeneiCel.7619.

3D structure databases

ProteinModelPortaliQ21122.
SMRiQ21122. Positions 29-439.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.K02B2.1.

Proteomic databases

EPDiQ21122.
PaxDbiQ21122.
PRIDEiQ21122.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiK02B2.1; K02B2.1; WBGene00019295.
GeneIDi177363.
KEGGicel:CELE_K02B2.1.
UCSCiK02B2.1. c. elegans.

Organism-specific databases

CTDi177363.
WormBaseiK02B2.1; CE30082; WBGene00019295; pfkb-1.2.

Phylogenomic databases

eggNOGiKOG0234. Eukaryota.
COG0406. LUCA.
GeneTreeiENSGT00390000018751.
HOGENOMiHOG000181112.
InParanoidiQ21122.
OMAiADEMPYL.
OrthoDBiEOG091G0A43.
PhylomeDBiQ21122.

Enzyme and pathway databases

ReactomeiR-CEL-70171. Glycolysis.
R-CEL-70263. Gluconeogenesis.

Miscellaneous databases

PROiQ21122.

Gene expression databases

BgeeiWBGene00019295.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR027417. P-loop_NTPase.
IPR001345. PG/BPGM_mutase_AS.
[Graphical view]
PANTHERiPTHR10606. PTHR10606. 1 hit.
PfamiPF01591. 6PF2K. 1 hit.
PF00300. His_Phos_1. 1 hit.
[Graphical view]
PRINTSiPR00991. 6PFRUCTKNASE.
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53254. SSF53254. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiF2612_CAEEL
AccessioniPrimary (citable) accession number: Q21122
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 6, 2002
Last modified: September 7, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.