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Protein

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase

Gene

pfkb-1.2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Synthesis and degradation of fructose 2,6-bisphosphate.By similarity

Catalytic activityi

Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69Fructose 6-phosphateBy similarity1
Binding sitei98Fructose 6-phosphateBy similarity1
Active sitei124Sequence analysis1
Binding sitei126Fructose 6-phosphateBy similarity1
Binding sitei132Fructose 6-phosphateBy similarity1
Active sitei154Sequence analysis1
Binding sitei168Fructose 6-phosphateBy similarity1
Binding sitei190Fructose 6-phosphateBy similarity1
Binding sitei194Fructose 6-phosphateBy similarity1
Binding sitei252Fructose 2,6-bisphosphateBy similarity1
Sitei252Transition state stabilizerBy similarity1
Active sitei253Tele-phosphohistidine intermediateBy similarity1
Binding sitei259Fructose 2,6-bisphosphateBy similarity1
Sitei259Transition state stabilizerBy similarity1
Binding sitei265Fructose 2,6-bisphosphate; via amide nitrogenBy similarity1
Active sitei324Proton donor/acceptorBy similarity1
Binding sitei335Fructose 2,6-bisphosphateBy similarity1
Binding sitei349Fructose 2,6-bisphosphateBy similarity1
Binding sitei353Fructose 2,6-bisphosphateBy similarity1
Binding sitei364Fructose 2,6-bisphosphateBy similarity1
Sitei389Transition state stabilizerBy similarity1
Binding sitei390Fructose 2,6-bisphosphateBy similarity1
Binding sitei394Fructose 2,6-bisphosphateBy similarity1
Binding sitei426ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 44ATPBy similarity9
Nucleotide bindingi163 – 168ATPBy similarity6
Nucleotide bindingi346 – 349ATPBy similarity4
Nucleotide bindingi390 – 394ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-70171. Glycolysis.
R-CEL-70263. Gluconeogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phosphofructo-2-kinase/fructose-2,6-bisphosphataseImported
Short name:
6PF-2-K/Fru-2,6-P2ase
Short name:
PFK/FBPase
Including the following 2 domains:
Fructose-2,6-bisphosphatase (EC:3.1.3.46)
Gene namesi
Name:pfkb-1.2Imported
ORF Names:K02B2.1Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiK02B2.1; CE30082; WBGene00019295; pfkb-1.2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001799741 – 4576-phosphofructo-2-kinase/fructose-2,6-bisphosphataseCuratedAdd BLAST457

Proteomic databases

EPDiQ21122.
PaxDbiQ21122.
PeptideAtlasiQ21122.

Expressioni

Gene expression databases

BgeeiWBGene00019295.

Interactioni

Protein-protein interaction databases

STRINGi6239.K02B2.1.

Structurei

3D structure databases

ProteinModelPortaliQ21122.
SMRiQ21122.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 2446-phosphofructo-2-kinaseAdd BLAST244
Regioni245 – 457Fructose-2,6-bisphosphataseAdd BLAST213

Sequence similaritiesi

In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated

Phylogenomic databases

eggNOGiKOG0234. Eukaryota.
COG0406. LUCA.
GeneTreeiENSGT00390000018751.
HOGENOMiHOG000181112.
InParanoidiQ21122.
OMAiADEMPYL.
OrthoDBiEOG091G0A43.
PhylomeDBiQ21122.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR027417. P-loop_NTPase.
IPR001345. PG/BPGM_mutase_AS.
[Graphical view]
PANTHERiPTHR10606. PTHR10606. 1 hit.
PfamiPF01591. 6PF2K. 1 hit.
PF00300. His_Phos_1. 1 hit.
[Graphical view]
PRINTSiPR00991. 6PFRUCTKNASE.
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53254. SSF53254. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q21122-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIPPGLETT KRKVAHSDEH GFSDQVRVPN VIVMVGLPAR GKTYISKKLC
60 70 80 90 100
RYLKWTGFTT KVFNVGEYRR SDANAADAIH GANASFFSPN NADALKVRAE
110 120 130 140 150
SARRAMEDMA DYLNSGTGGV AIFDATNTTK DRRRIIIDFC KKQRLRCFFI
160 170 180 190 200
ESVCDDPAII DCNVTDVKVN SPDYKGLMTA EQAKEDFMNR IENYKKQYEP
210 220 230 240 250
LDESEDESLS FIKVINAGRS FKVHQVRGHV QSRVVYFLMN IHLLPRSIYL
260 270 280 290 300
TRHGQSEYNA MGRLGGDSPL TEDGQKYASA LADFFEEEEV PGLRVWCSQK
310 320 330 340 350
VRAAQTAQHL KPDFHTEYWK ALDELDAGIC EGLTYEDILQ RYPKQADDRA
360 370 380 390 400
TDKYHYRYPS GESYEDVVSR LEPVIMELER QANVLVVSHQ AVLRCVLAYF
410 420 430 440 450
YDRPLSELPY IDIPLHSLVK LTPRAYHCDS TIYALDLESG EWTETSDQLP

LCDSPRD
Length:457
Mass (Da):52,091
Last modified:June 6, 2002 - v2
Checksum:iE04A9A892378E413
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080181 Genomic DNA. Translation: CCD61801.1.
RefSeqiNP_500893.2. NM_068492.5.
UniGeneiCel.7619.

Genome annotation databases

EnsemblMetazoaiK02B2.1; K02B2.1; WBGene00019295.
GeneIDi177363.
KEGGicel:CELE_K02B2.1.
UCSCiK02B2.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080181 Genomic DNA. Translation: CCD61801.1.
RefSeqiNP_500893.2. NM_068492.5.
UniGeneiCel.7619.

3D structure databases

ProteinModelPortaliQ21122.
SMRiQ21122.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.K02B2.1.

Proteomic databases

EPDiQ21122.
PaxDbiQ21122.
PeptideAtlasiQ21122.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiK02B2.1; K02B2.1; WBGene00019295.
GeneIDi177363.
KEGGicel:CELE_K02B2.1.
UCSCiK02B2.1. c. elegans.

Organism-specific databases

CTDi177363.
WormBaseiK02B2.1; CE30082; WBGene00019295; pfkb-1.2.

Phylogenomic databases

eggNOGiKOG0234. Eukaryota.
COG0406. LUCA.
GeneTreeiENSGT00390000018751.
HOGENOMiHOG000181112.
InParanoidiQ21122.
OMAiADEMPYL.
OrthoDBiEOG091G0A43.
PhylomeDBiQ21122.

Enzyme and pathway databases

ReactomeiR-CEL-70171. Glycolysis.
R-CEL-70263. Gluconeogenesis.

Miscellaneous databases

PROiQ21122.

Gene expression databases

BgeeiWBGene00019295.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR027417. P-loop_NTPase.
IPR001345. PG/BPGM_mutase_AS.
[Graphical view]
PANTHERiPTHR10606. PTHR10606. 1 hit.
PfamiPF01591. 6PF2K. 1 hit.
PF00300. His_Phos_1. 1 hit.
[Graphical view]
PRINTSiPR00991. 6PFRUCTKNASE.
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53254. SSF53254. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiF2612_CAEEL
AccessioniPrimary (citable) accession number: Q21122
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 6, 2002
Last modified: November 2, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.