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Protein

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase

Gene

pfkb-1.2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Synthesis and degradation of fructose 2,6-bisphosphate.By similarity

Catalytic activityi

Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69Fructose 6-phosphateBy similarity1
Binding sitei98Fructose 6-phosphateBy similarity1
Active sitei124Sequence analysis1
Binding sitei126Fructose 6-phosphateBy similarity1
Binding sitei132Fructose 6-phosphateBy similarity1
Active sitei154Sequence analysis1
Binding sitei168Fructose 6-phosphateBy similarity1
Binding sitei190Fructose 6-phosphateBy similarity1
Binding sitei194Fructose 6-phosphateBy similarity1
Binding sitei252Fructose 2,6-bisphosphateBy similarity1
Sitei252Transition state stabilizerBy similarity1
Active sitei253Tele-phosphohistidine intermediateBy similarity1
Binding sitei259Fructose 2,6-bisphosphateBy similarity1
Sitei259Transition state stabilizerBy similarity1
Binding sitei265Fructose 2,6-bisphosphate; via amide nitrogenBy similarity1
Active sitei324Proton donor/acceptorBy similarity1
Binding sitei335Fructose 2,6-bisphosphateBy similarity1
Binding sitei349Fructose 2,6-bisphosphateBy similarity1
Binding sitei353Fructose 2,6-bisphosphateBy similarity1
Binding sitei364Fructose 2,6-bisphosphateBy similarity1
Sitei389Transition state stabilizerBy similarity1
Binding sitei390Fructose 2,6-bisphosphateBy similarity1
Binding sitei394Fructose 2,6-bisphosphateBy similarity1
Binding sitei426ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 44ATPBy similarity9
Nucleotide bindingi163 – 168ATPBy similarity6
Nucleotide bindingi346 – 349ATPBy similarity4
Nucleotide bindingi390 – 394ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Kinase, Multifunctional enzyme, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-70171 Glycolysis

Names & Taxonomyi

Protein namesi
Recommended name:
6-phosphofructo-2-kinase/fructose-2,6-bisphosphataseImported
Short name:
6PF-2-K/Fru-2,6-P2ase
Short name:
PFK/FBPase
Including the following 2 domains:
Fructose-2,6-bisphosphatase (EC:3.1.3.46)
Gene namesi
Name:pfkb-1.2Imported
ORF Names:K02B2.1Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiK02B2.1 ; CE30082 ; WBGene00019295 ; pfkb-1.2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001799741 – 4576-phosphofructo-2-kinase/fructose-2,6-bisphosphataseCuratedAdd BLAST457

Proteomic databases

EPDiQ21122
PaxDbiQ21122
PeptideAtlasiQ21122
PRIDEiQ21122

Expressioni

Gene expression databases

BgeeiWBGene00019295

Interactioni

Protein-protein interaction databases

STRINGi6239.K02B2.1

Structurei

3D structure databases

ProteinModelPortaliQ21122
SMRiQ21122
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 2446-phosphofructo-2-kinaseAdd BLAST244
Regioni245 – 457Fructose-2,6-bisphosphataseAdd BLAST213

Sequence similaritiesi

In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated

Phylogenomic databases

eggNOGiKOG0234 Eukaryota
COG0406 LUCA
GeneTreeiENSGT00390000018751
HOGENOMiHOG000181112
InParanoidiQ21122
OMAiHDSCEWL
OrthoDBiEOG091G0A43
PhylomeDBiQ21122

Family and domain databases

CDDicd07067 HP_PGM_like, 1 hit
Gene3Di3.40.50.1240, 1 hit
InterProiView protein in InterPro
IPR003094 6Pfruct_kin
IPR013079 6Phosfructo_kin
IPR013078 His_Pase_superF_clade-1
IPR029033 His_PPase_superfam
IPR027417 P-loop_NTPase
IPR001345 PG/BPGM_mutase_AS
PANTHERiPTHR10606 PTHR10606, 1 hit
PfamiView protein in Pfam
PF01591 6PF2K, 1 hit
PF00300 His_Phos_1, 1 hit
PRINTSiPR00991 6PFRUCTKNASE
SMARTiView protein in SMART
SM00855 PGAM, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
SSF53254 SSF53254, 1 hit
PROSITEiView protein in PROSITE
PS00175 PG_MUTASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q21122-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIPPGLETT KRKVAHSDEH GFSDQVRVPN VIVMVGLPAR GKTYISKKLC
60 70 80 90 100
RYLKWTGFTT KVFNVGEYRR SDANAADAIH GANASFFSPN NADALKVRAE
110 120 130 140 150
SARRAMEDMA DYLNSGTGGV AIFDATNTTK DRRRIIIDFC KKQRLRCFFI
160 170 180 190 200
ESVCDDPAII DCNVTDVKVN SPDYKGLMTA EQAKEDFMNR IENYKKQYEP
210 220 230 240 250
LDESEDESLS FIKVINAGRS FKVHQVRGHV QSRVVYFLMN IHLLPRSIYL
260 270 280 290 300
TRHGQSEYNA MGRLGGDSPL TEDGQKYASA LADFFEEEEV PGLRVWCSQK
310 320 330 340 350
VRAAQTAQHL KPDFHTEYWK ALDELDAGIC EGLTYEDILQ RYPKQADDRA
360 370 380 390 400
TDKYHYRYPS GESYEDVVSR LEPVIMELER QANVLVVSHQ AVLRCVLAYF
410 420 430 440 450
YDRPLSELPY IDIPLHSLVK LTPRAYHCDS TIYALDLESG EWTETSDQLP

LCDSPRD
Length:457
Mass (Da):52,091
Last modified:June 6, 2002 - v2
Checksum:iE04A9A892378E413
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080181 Genomic DNA Translation: CCD61801.1
RefSeqiNP_500893.2, NM_068492.5
UniGeneiCel.7619

Genome annotation databases

EnsemblMetazoaiK02B2.1; K02B2.1; WBGene00019295
GeneIDi177363
KEGGicel:CELE_K02B2.1
UCSCiK02B2.1 c. elegans

Similar proteinsi

Entry informationi

Entry nameiF2612_CAEEL
AccessioniPrimary (citable) accession number: Q21122
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 6, 2002
Last modified: May 23, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

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