Zinc metalloproteinase nas-34 precursor

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Q21059 (NAS34_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 85. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Zinc metalloproteinase nas-34
EC=3.4.24.21

Alternative name(s):
Defective hatching protein 1
Nematode astacin 34

Gene names

Name:	hch-1
Synonyms:	nas-34
ORF Names:	F40E10.1

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	605 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable metalloprotease required for normal hatching and migration of neuroblasts. May act by degrading eggshells proteins at hatching. Ref.1
Catalytic activity	Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted Potential.
Tissue specificity	Expressed in hypodermal cells. First expressed in the dorsal and lateral surface area of the middle and posterior region of embryos. At later stages, it localizes to lateral surface regions, probably corresponding to hypodermal seam cells. In L1 larvae, it is expressed in seam cells and in a few cells anterior to the nerve ring. Ref.1
Developmental stage	In embryos, it is first expressed just before elongation. Ref.1
Sequence similarities	Belongs to the peptidase M12A family. Contains 1 CUB domain. Contains 1 EGF-like domain. Contains 1 TSP type-1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	EGF-like domain Signal
Ligand	Metal-binding Zinc
Molecular function	Developmental protein Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	hatching Inferred from mutant phenotype Ref.1. Source: WormBase neuron migration Inferred from mutant phenotype Ref.1. Source: WormBase proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

Potential

Chain

20 – 605

586

Zinc metalloproteinase nas-34

PRO_0000028938

Regions

Domain

317 – 357

EGF-like

Domain

366 – 469

104

CUB

Domain

525 – 566

TSP type-1

Compositional bias

173 – 178

Poly-Ser

Compositional bias

479 – 487

Poly-Thr

Compositional bias

498 – 502

Poly-Thr

Compositional bias

505 – 508

Poly-Thr

Compositional bias

511 – 518

Poly-Thr

Sites

Active site

220

By similarity

Metal binding

219

Zinc; catalytic By similarity

Metal binding

223

Zinc; catalytic By similarity

Metal binding

229

Zinc; catalytic By similarity

Amino acid modifications

Glycosylation

322

N-linked (GlcNAc...) Ref.4

Disulfide bond

321 ↔ 331

By similarity

Disulfide bond

325 ↔ 345

By similarity

Disulfide bond

347 ↔ 356

By similarity

Disulfide bond

366 ↔ 388

By similarity

Disulfide bond

415 ↔ 436

By similarity

Disulfide bond

531 ↔ 551

By similarity

Disulfide bond

537 ↔ 560

By similarity

Disulfide bond

541 ↔ 565

By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q21059 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: E32E67A0F2674809
Show »

FASTA

605

66,259

        10         20         30         40         50         60 
MVSYWPVLIV LCLLPICHAK SYFADFVNGK GPFKQADALK FMDKMTILNK LQADILGIPQ 

        70         80         90        100        110        120 
PDEFSALDFE DKIESKPDEI PYLFEGDMVL TDEQMDLIIK NVRDQYWARK SSTNEFLYAI 

       130        140        150        160        170        180 
RGKRSMTSFL SERWSFPVPY YIDTSSGVNT NAVLAGVAKW EQETCARFTR LNSYSSSSRQ 

       190        200        210        220        230        240 
NALRFISGNG CYSNIGKVSR FPQDVSIGWG CTSLGTVCHE IGHALGFYHE QARYDRDDYV 

       250        260        270        280        290        300 
SILTQNIQDM YLSQFTKQSA SSMVDYGVGY DYGSVMHYDQ AAFSSTGGNT IATRDPNFQA 

       310        320        330        340        350        360 
TIGQRVAPSF ADVKRINFAY CNSTCSNYLD CQNGGYINPN DCNNCKCPPG FGGQLCDVAG 

       370        380        390        400        410        420 
TNSNGCGAGD ITATSSIQTI SASGALTCNY VIKAPVGAKV YFQMTAATFS RYSPCTTNYL 

       430        440        450        460        470        480 
EINYGRDFSR VGARFCASYP TISLSETNTL VVIYKGVNGA RFSLNYRYDP VTFSTSAPTT 

       490        500        510        520        530        540 
TSTTTTTAPI TVPTVSPTTT TTRQTTTTAR TSTTTTTTQA PPTTTTSTSQ CASWSACSAQ 

       550        560        570        580        590        600 
CGGCGTQSRR CGTYVETVYC NTNPCTGGYC CRPFFYVTSF GTGYCRRPGA DTPAAPQRYV 


EQRKG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"hch-1, a gene required for normal hatching and normal migration of a neuroblast in C. elegans, encodes a protein related to TOLLOID and BMP-1." Hishida R., Ishihara T., Kondo K., Katsura I. EMBO J. 15:4111-4122(1996) [PubMed: 8861940] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"The astacin protein family in Caenorhabditis elegans." Moehrlen F., Hutter H., Zwilling R. Eur. J. Biochem. 270:4909-4920(2003) [PubMed: 14653817] [Abstract] Cited for: NOMENCLATURE.
[4]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-322, MASS SPECTROMETRY. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D85744 mRNA. Translation: BAA12861.1. Z69792 Genomic DNA. Translation: CAB61002.1.
PIR	S71630.
RefSeq	NP_510440.1. NM_078039.4.
UniGene	Cel.23554.
3D structure databases
ProteinModelPortal	Q21059.
SMR	Q21059. Positions 78-327, 381-469, 525-565.
ModBase	Search...
Protein-protein interaction databases
STRING	Q21059.
Protein family/group databases
MEROPS	M12.A25.
Proteomic databases
PRIDE	Q21059.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F40E10.1; F40E10.1; F40E10.1.
GeneID	181564.
KEGG	cel:F40E10.1.
NMPDR	fig\|6239.3.peg.25288.
UCSC	F40E10.1. c. elegans.
Organism-specific databases
CTD	181564.
WormBase	F40E10.1; CE24964; WBGene00001828; hch-1.
Phylogenomic databases
eggNOG	meNOG26466.
GeneTree	EMGT00050000002037.
HOGENOM	HBG389352.
InParanoid	Q21059.
OMA	FLYAIRG.
PhylomeDB	Q21059.
Gene expression databases
ArrayExpress	Q21059.
Family and domain databases
InterPro	IPR000859. CUB. IPR006210. EGF-like. IPR013032. EGF-like_reg_CS. IPR000742. EGF_3. IPR024079. MetalloPept_cat_dom. IPR001506. Peptidase_M12A. IPR017050. Peptidase_M12A_nem. IPR006026. Peptidase_Metallo. IPR000884. Thrombospondin_1_rpt. [Graphical view]
Gene3D	G3DSA:2.60.120.290. CUB. 1 hit. G3DSA:3.40.390.10. G3DSA:3.40.390.10. 1 hit.
KO	K08076.
Pfam	PF01400. Astacin. 1 hit. PF00431. CUB. 1 hit. [Graphical view]
PIRSF	PIRSF036365. Astacin_nematoda. 1 hit.
PRINTS	PR00480. ASTACIN.
SMART	SM00042. CUB. 1 hit. SM00181. EGF. 1 hit. SM00235. ZnMc. 1 hit. [Graphical view]
SUPFAM	SSF49854. CUB. 1 hit.
PROSITE	PS01180. CUB. 1 hit. PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 1 hit. PS50092. TSP1. 1 hit. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	914458.

Entry information

Entry name

NAS34_CAEEL

Accession

Primary (citable) accession number: Q21059

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents