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Protein

Zinc metalloproteinase nas-34

Gene

hch-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable metalloprotease required for normal hatching and migration of neuroblasts. May act by degrading eggshells proteins at hatching.1 Publication

Catalytic activityi

Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi219 – 2191Zinc; catalyticPROSITE-ProRule annotation
Active sitei220 – 2201PROSITE-ProRule annotation
Metal bindingi223 – 2231Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi229 – 2291Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • hatching Source: WormBase
  • molting cycle, collagen and cuticulin-based cuticle Source: InterPro
  • neuron migration Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.A25.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase nas-34 (EC:3.4.24.21)
Alternative name(s):
Defective hatching protein 1
Nematode astacin 34
Gene namesi
Name:hch-1
Synonyms:nas-34
ORF Names:F40E10.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiF40E10.1; CE24964; WBGene00001828; hch-1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 605586Zinc metalloproteinase nas-34PRO_0000028938Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi321 ↔ 331By similarity
Glycosylationi322 – 3221N-linked (GlcNAc...)1 Publication
Disulfide bondi325 ↔ 345By similarity
Disulfide bondi347 ↔ 356By similarity
Disulfide bondi366 ↔ 388By similarity
Disulfide bondi415 ↔ 436By similarity
Disulfide bondi531 ↔ 551By similarity
Disulfide bondi537 ↔ 560By similarity
Disulfide bondi541 ↔ 565By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ21059.
PRIDEiQ21059.

Expressioni

Tissue specificityi

Expressed in hypodermal cells. First expressed in the dorsal and lateral surface area of the middle and posterior region of embryos. At later stages, it localizes to lateral surface regions, probably corresponding to hypodermal seam cells. In L1 larvae, it is expressed in seam cells and in a few cells anterior to the nerve ring.1 Publication

Developmental stagei

In embryos, it is first expressed just before elongation.1 Publication

Gene expression databases

BgeeiWBGene00001828.

Interactioni

Protein-protein interaction databases

STRINGi6239.F40E10.1.

Structurei

3D structure databases

ProteinModelPortaliQ21059.
SMRiQ21059. Positions 138-320.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini317 – 35741EGF-likePROSITE-ProRule annotationAdd
BLAST
Domaini366 – 469104CUBPROSITE-ProRule annotationAdd
BLAST
Domaini525 – 56642TSP type-1PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi173 – 1786Poly-Ser
Compositional biasi479 – 4879Poly-Thr
Compositional biasi498 – 5025Poly-Thr
Compositional biasi505 – 5084Poly-Thr
Compositional biasi511 – 5188Poly-Thr

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated
Contains 1 CUB domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 TSP type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119227.
HOGENOMiHOG000018339.
InParanoidiQ21059.
KOiK08076.
OMAiFLYAIRG.
OrthoDBiEOG091G0N5F.
PhylomeDBiQ21059.

Family and domain databases

Gene3Di2.60.120.290. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000859. CUB_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR017050. Metallopeptidase_nem.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
[Graphical view]
PIRSFiPIRSF036365. Astacin_nematoda. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00042. CUB. 1 hit.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
PROSITEiPS01180. CUB. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS50092. TSP1. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q21059-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSYWPVLIV LCLLPICHAK SYFADFVNGK GPFKQADALK FMDKMTILNK
60 70 80 90 100
LQADILGIPQ PDEFSALDFE DKIESKPDEI PYLFEGDMVL TDEQMDLIIK
110 120 130 140 150
NVRDQYWARK SSTNEFLYAI RGKRSMTSFL SERWSFPVPY YIDTSSGVNT
160 170 180 190 200
NAVLAGVAKW EQETCARFTR LNSYSSSSRQ NALRFISGNG CYSNIGKVSR
210 220 230 240 250
FPQDVSIGWG CTSLGTVCHE IGHALGFYHE QARYDRDDYV SILTQNIQDM
260 270 280 290 300
YLSQFTKQSA SSMVDYGVGY DYGSVMHYDQ AAFSSTGGNT IATRDPNFQA
310 320 330 340 350
TIGQRVAPSF ADVKRINFAY CNSTCSNYLD CQNGGYINPN DCNNCKCPPG
360 370 380 390 400
FGGQLCDVAG TNSNGCGAGD ITATSSIQTI SASGALTCNY VIKAPVGAKV
410 420 430 440 450
YFQMTAATFS RYSPCTTNYL EINYGRDFSR VGARFCASYP TISLSETNTL
460 470 480 490 500
VVIYKGVNGA RFSLNYRYDP VTFSTSAPTT TSTTTTTAPI TVPTVSPTTT
510 520 530 540 550
TTRQTTTTAR TSTTTTTTQA PPTTTTSTSQ CASWSACSAQ CGGCGTQSRR
560 570 580 590 600
CGTYVETVYC NTNPCTGGYC CRPFFYVTSF GTGYCRRPGA DTPAAPQRYV

EQRKG
Length:605
Mass (Da):66,259
Last modified:November 1, 1996 - v1
Checksum:iE32E67A0F2674809
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85744 mRNA. Translation: BAA12861.1.
Z69792 Genomic DNA. Translation: CAB61002.1.
PIRiS71630.
RefSeqiNP_510440.1. NM_078039.5.
UniGeneiCel.23554.

Genome annotation databases

EnsemblMetazoaiF40E10.1; F40E10.1; WBGene00001828.
GeneIDi181564.
KEGGicel:CELE_F40E10.1.
UCSCiF40E10.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85744 mRNA. Translation: BAA12861.1.
Z69792 Genomic DNA. Translation: CAB61002.1.
PIRiS71630.
RefSeqiNP_510440.1. NM_078039.5.
UniGeneiCel.23554.

3D structure databases

ProteinModelPortaliQ21059.
SMRiQ21059. Positions 138-320.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.F40E10.1.

Protein family/group databases

MEROPSiM12.A25.

Proteomic databases

PaxDbiQ21059.
PRIDEiQ21059.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF40E10.1; F40E10.1; WBGene00001828.
GeneIDi181564.
KEGGicel:CELE_F40E10.1.
UCSCiF40E10.1. c. elegans.

Organism-specific databases

CTDi181564.
WormBaseiF40E10.1; CE24964; WBGene00001828; hch-1.

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119227.
HOGENOMiHOG000018339.
InParanoidiQ21059.
KOiK08076.
OMAiFLYAIRG.
OrthoDBiEOG091G0N5F.
PhylomeDBiQ21059.

Miscellaneous databases

PROiQ21059.

Gene expression databases

BgeeiWBGene00001828.

Family and domain databases

Gene3Di2.60.120.290. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000859. CUB_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR017050. Metallopeptidase_nem.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
[Graphical view]
PIRSFiPIRSF036365. Astacin_nematoda. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00042. CUB. 1 hit.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
PROSITEiPS01180. CUB. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS50092. TSP1. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAS34_CAEEL
AccessioniPrimary (citable) accession number: Q21059
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.