Reviewed, UniProtKB/Swiss-Prot Q20YV7 (METK2_RHOPB)

Names and origin

Protein names

Recommended name:
    S-adenosylmethionine synthetase 2
    EC=2.5.1.6
Alternative name(s):
    Methionine adenosyltransferase 2
    AdoMet synthetase 2
    MAT 2

Gene names

Name:	metK2
Ordered Locus Names:	RPC_4154

Organism

Rhodopseudomonas palustris (strain BisB18) [Complete proteome] [HAMAP]

Taxonomic identifier

316056 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity. HAMAP MF_00086
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine. HAMAP MF_00086
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. HAMAP MF_00086 Binds 1 potassium ion per subunit By similarity. HAMAP MF_00086
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1. HAMAP MF_00086
Subunit structure	Homotetramer By similarity. HAMAP MF_00086
Subcellular location	Cytoplasm HAMAP MF_00086.
Sequence similarities	Belongs to the AdoMet synthetase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP methionine adenosyltransferase activity Inferred from electronic annotation. Source: HAMAP potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	S-adenosylmethionine synthetase 2 HAMAP MF_00086		PRO_0000241028
Regions
	Nucleotide binding	272 – 279	8	ATP Potential
Sites
	Metal binding	18	1	Magnesium By similarity
	Metal binding	51	1	Potassium By similarity
	Metal binding	276	1	Potassium By similarity
	Metal binding	284	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q20YV7-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: CCF071CEE4532A47	FASTA	398	43,415
	10 20 30 40 50 60 MRASYLFTSE SVSEGHPDKV CDRISDEIVD LFFREGPKAG IDPWAIRAAC ETLATTNKVV 70 80 90 100 110 120 IAGETRGPAS VTNEHIEHVV REAIKDIGYE QDGFHWKTAD IEILLHPQSA DIAQGVDALQ 130 140 150 160 170 180 PGTNQEEGAG DQGIMFGYAT NETPDLMPAP IFYAHKILRL ISEARHSGKE KVLGPDSKSQ 190 200 210 220 230 240 VTIQYENGKP VGVREIVVSH QHLVEDMTSN QVRERVEPYV REALPEGWIT DKTIWHINPT 250 260 270 280 290 300 GKFFIGGPDG DTGLTGRKII VDTYGGAAPH GGGAFSGKDP TKVDRSAAYA SRYLAKNIVA 310 320 330 340 350 360 AGLADRCTLQ LAYAIGVARP LSIYIDTHGT GKVTEDKLEK AVAEAMDLTP RGIRKHLDLN 370 380 390 KPIYARTSSY GHFGRTPDAE GGFSWEKTDL AEALKRAI

References

[1]

"Complete sequence of Rhodopseudomonas palustris BisB18." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Pelletier D.A., Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000301 Genomic DNA. Translation: ABD89679.1.
RefSeq	YP_533998.1.
3D structure databases
SMR	Q20YV7. Positions 3-398.
ModBase	Search...
Protein-protein interaction databases
STRING	Q20YV7.
Genome annotation databases
GeneID	3973989.
GenomeReviews	Gene locus RPC_4154 in contig CP000301_GR.
KEGG	rpc:RPC_4154.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0192.
HOGENOM	HBG443662.
OMA	QDGFHWK.
Enzyme and pathway databases
BioCyc	RPAL316056:RPC_4154-MONOMER.
Family and domain databases
HAMAP	MF_00086. S-AdoMet_synth1. [Tree]
InterPro	IPR002133. S-AdoMet_synthetase. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
TIGRFAMs	TIGR01034. metK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHETASE_1. 1 hit. PS00377. ADOMET_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METK2_RHOPB

Accession

Primary (citable) accession number: Q20YV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	April 18, 2006
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)