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Reviewed, UniProtKB/Swiss-Prot Q20YV7 (METK2_RHOPB)

Last modified November 3, 2009. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    S-adenosylmethionine synthetase 2
    EC=2.5.1.6
Alternative name(s):
    Methionine adenosyltransferase 2
    AdoMet synthetase 2
    MAT 2
Gene names
Name: metK2
Ordered Locus Names: RPC_4154
OrganismRhodopseudomonas palustris (strain BisB18) [Complete proteome] [HAMAP]
Taxonomic identifier316056 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas

Protein attributes

Sequence length398 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity.

Catalytic activity

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine. HAMAP MF_00086

Cofactor

Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity.

Binds 1 potassium ion per subunit By similarity.

Pathway

Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1. HAMAP MF_00086

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm. HAMAP MF_00086

Sequence similarities

Belongs to the AdoMet synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 398398S-adenosylmethionine synthetase 2 HAMAP MF_00086
PRO_0000241028

Regions

Nucleotide binding272 – 2798ATP Potential

Sites

Metal binding181Magnesium By similarity
Metal binding511Potassium By similarity
Metal binding2761Potassium By similarity
Metal binding2841Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q20YV7-1 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: CCF071CEE4532A47

FASTA39843,415
        10         20         30         40         50         60 
MRASYLFTSE SVSEGHPDKV CDRISDEIVD LFFREGPKAG IDPWAIRAAC ETLATTNKVV 

        70         80         90        100        110        120 
IAGETRGPAS VTNEHIEHVV REAIKDIGYE QDGFHWKTAD IEILLHPQSA DIAQGVDALQ 

       130        140        150        160        170        180 
PGTNQEEGAG DQGIMFGYAT NETPDLMPAP IFYAHKILRL ISEARHSGKE KVLGPDSKSQ 

       190        200        210        220        230        240 
VTIQYENGKP VGVREIVVSH QHLVEDMTSN QVRERVEPYV REALPEGWIT DKTIWHINPT 

       250        260        270        280        290        300 
GKFFIGGPDG DTGLTGRKII VDTYGGAAPH GGGAFSGKDP TKVDRSAAYA SRYLAKNIVA 

       310        320        330        340        350        360 
AGLADRCTLQ LAYAIGVARP LSIYIDTHGT GKVTEDKLEK AVAEAMDLTP RGIRKHLDLN 

       370        380        390 
KPIYARTSSY GHFGRTPDAE GGFSWEKTDL AEALKRAI 

« Hide

References

[1]"Complete sequence of Rhodopseudomonas palustris BisB18."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Pelletier D.A., Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

CP000301 Genomic DNA. Translation: ABD89679.1.
RefSeqYP_533998.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ20YV7.

Genome annotation databases

GeneID3973989.
GenomeReviewsGene locus RPC_4154 in contig CP000301_GR.
KEGGrpc:RPC_4154.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ20YV7.
OMANKDEGAG.

Enzyme and pathway databases

BioCycRPAL316056:RPC_4154-MON.

Family and domain databases

HAMAPMF_00086.
[Tree]
InterProIPR002133. S-AdoMet_synthetase.
[Graphical view]
PANTHERPTHR11964. S-AdoMet_synt. 1 hit.
PfamPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFPIRSF000497. MAT. 1 hit.
TIGRFAMsTIGR01034. metK. 1 hit.
PROSITEPS00376. ADOMET_SYNTHETASE_1. 1 hit.
PS00377. ADOMET_SYNTHETASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMETK2_RHOPB
AccessionPrimary (citable) accession number: Q20YV7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: April 18, 2006
Last modified: November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents