Q20YL8 (CHEB1_RHOPB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase 1
EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	RPC_4244

Organism

Rhodopseudomonas palustris (strain BisB18) [Complete proteome] [HAMAP]

Taxonomic identifier

316056 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 389	389	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000264306
Regions
	Domain	20 – 138	119	Response regulatory
	Domain	193 – 387	195	CheB-type methylesterase
Sites
	Active site	205	1	By similarity
	Active site	233	1	By similarity
	Active site	329	1	By similarity
Amino acid modifications
	Modified residue	71	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q20YL8 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: A5EF7884284DD7D2	FASTA	389	40,920
	10 20 30 40 50 60 MSIAVASASI PEPVRYEPLR VMVVDDSVVI RGLISRWIEA EPDMVVAASL RTGLDAVNQV 70 80 90 100 110 120 ERVKPDVALL DIEMPELDGI SALPQLLAKQ RNLIIIMAST LTRRNAEISF KALSLGAADY 130 140 150 160 170 180 IPKPESTREA SAAEIFRHDL LQKVRSLGAR VRRKVQTGPG AVAAPAIVRA REPAPVPAAG 190 200 210 220 230 240 HAPAVPLLRR ALSLTAPRVL LIGSSTGGPQ ALMTLVADIG AVIDRFPVLI TQHMPPTFTT 250 260 270 280 290 300 ILAEHLARAS RRPAHEGIDG EIIKPGQIYL APGGKHMRVA RQGGNTVIAL DDGPAVNFCK 310 320 330 340 350 360 PAVDPLFISA IEVWQSAIMA VVLTGMGSDG MRGGKDVVAA GGSVIAQDEA SSVVWGMPGA 370 380 AANAGICAAI LPLNQIGQKL VRVFAGDRS

References

[1]

"Complete sequence of Rhodopseudomonas palustris BisB18." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Pelletier D.A., Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BisB18.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000301 Genomic DNA. Translation: ABD89768.1.
RefSeq	YP_534087.1. NC_007925.1.
3D structure databases
ProteinModelPortal	Q20YL8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q20YL8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3971586.
GenomeReviews	Gene locus RPC_4244 in contig CP000301_GR.
KEGG	rpc:RPC_4244.
PATRIC	23273324. VBIRhoPal29154_4336.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	IIIMAST.
PhylomeDB	Q20YL8.
ProtClustDB	PRK00742.
Enzyme and pathway databases
BioCyc	RPAL316056:RPC_4244-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_RHOPB

Accession

Primary (citable) accession number: Q20YL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	April 18, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program