Q20XB0 (LPXK_RHOPB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	RPC_4704

Organism

Rhodopseudomonas palustris (strain BisB18) [Complete proteome] [HAMAP]

Taxonomic identifier

316056 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_0000291235
Regions
	Nucleotide binding	51 – 58	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q20XB0 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 2AB24D12F47B3FAA	FASTA	343	35,971
	10 20 30 40 50 60 MREPGFWYRP PSLLSSLLRP LGAIYGAVAA HRMAQPGTAV GVPVLCIGNF HGGGAGKTPT 70 80 90 100 110 120 ALALARLLLE LGERPVVLSR GYGGRLHGPV SVDPGRHGAA DVGDEPLMMA RDIPVVVARD 130 140 150 160 170 180 RVAGAALARS RGASVILMDD GFQNPAVAKD AALIVIDGDR GLGNGYVIPA GPLRAPLPPQ 190 200 210 220 230 240 LARTDALIVV GRGRAADPVA AAVKDNGGLL LRARFLPAEA SLAALCDRPV LAFAGIGDPA 250 260 270 280 290 300 RFFATLRTAG INVAAERVFA DHHPYAKQDL AGLTEIAGRD GFTLVTTEKD LARLRSDPAH 310 320 330 340 AAFARSVVPF AVTLEFDDAK ALRDFVIEKL TQARDKHRRA GRS

References

[1]

"Complete sequence of Rhodopseudomonas palustris BisB18." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Pelletier D.A., Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BisB18.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000301 Genomic DNA. Translation: ABD90226.1.
RefSeq	YP_534545.1. NC_007925.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q20XB0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3972622.
GenomeReviews	Gene locus RPC_4704 in contig CP000301_GR.
KEGG	rpc:RPC_4704.
PATRIC	23274304. VBIRhoPal29154_4815.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1663.
HOGENOM	HBG663914.
OMA	VILMDDG.
ProtClustDB	PRK00652.
Enzyme and pathway databases
BioCyc	RPAL316056:RPC_4704-MONOMER.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_RHOPB

Accession

Primary (citable) accession number: Q20XB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	April 18, 2006
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program