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Q20IJ2

- Q20IJ2_9FLAV

UniProt

Q20IJ2 - Q20IJ2_9FLAV

Protein
Submitted name:

Polyprotein

Gene
N/A
Organism
Dengue virus 2
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (18 Apr 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).SAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei2547 – 25471S-adenosyl-L-homocysteineImported
    Binding sitei2578 – 25781S-adenosyl-L-homocysteineImported
    Binding sitei2596 – 25961S-adenosyl-L-homocysteine; via amide nitrogenImported
    Binding sitei2623 – 26231S-adenosyl-L-homocysteine; via amide nitrogenImported

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent helicase activity Source: InterPro
    3. double-stranded RNA binding Source: InterPro
    4. mRNA (guanine-N7-)-methyltransferase activity Source: InterPro
    5. mRNA (nucleoside-2'-O-)-methyltransferase activity Source: InterPro
    6. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    7. RNA helicase activity Source: InterPro
    8. serine-type endopeptidase activity Source: InterPro
    9. serine-type exopeptidase activity Source: InterPro
    10. structural molecule activity Source: InterPro

    GO - Biological processi

    1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
    2. viral RNA genome replication Source: InterPro
    3. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    HelicaseSAAS annotation, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymeraseSAAS annotation, Serine proteaseSAAS annotation, Transferase

    Keywords - Biological processi

    Fusion of virus membrane with host endosomal membraneSAAS annotation, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cellSAAS annotation, Viral penetration into host cytoplasm, Viral RNA replicationSAAS annotation, Virus entry into host cell

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-bindingSAAS annotation

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    PolyproteinImported
    OrganismiDengue virus 2Imported
    Taxonomic identifieri11060 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageFlaviviridaeFlavivirusDengue virus group

    Subcellular locationi

    GO - Cellular componenti

    1. host cell membrane Source: UniProtKB-KW
    2. integral component of membrane Source: UniProtKB-KW
    3. viral capsid Source: UniProtKB-KW
    4. viral envelope Source: InterPro

    Keywords - Cellular componenti

    Capsid proteinSAAS annotation, Host membraneSAAS annotation, Membrane, Virion

    PTM / Processingi

    Keywords - PTMi

    Disulfide bondSAAS annotation

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2P3QX-ray2.75A2495-2787[»]
    ProteinModelPortaliQ20IJ2.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ20IJ2.

    Family & Domainsi

    Sequence similaritiesi

    Contains RdRp catalytic domain.SAAS annotation
    Contains helicase ATP-binding domain.SAAS annotation
    Contains helicase C-terminal domain.SAAS annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helixSAAS annotation

    Family and domain databases

    Gene3Di2.60.40.350. 1 hit.
    2.60.98.10. 2 hits.
    3.30.387.10. 1 hit.
    3.40.50.150. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR011492. DEAD_Flavivir.
    IPR000069. Env_glycoprot_M_flavivir.
    IPR013755. Flav_gly_cen_dom_subdom1.
    IPR001122. Flavi_capsidC.
    IPR026470. Flavi_E_Stem/Anchor_dom.
    IPR001157. Flavi_NS1.
    IPR000752. Flavi_NS2A.
    IPR000487. Flavi_NS2B.
    IPR000404. Flavi_NS4A.
    IPR001528. Flavi_NS4B.
    IPR002535. Flavi_propep.
    IPR000336. Flavivir/Alphavir_Ig-like.
    IPR001850. Flavivirus_NS3_S7.
    IPR027287. Flavovir_Ig-like.
    IPR014412. Gen_Poly_FLV.
    IPR011998. Glycoprot_cen/dimer.
    IPR013754. GlyE_dim.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR014756. Ig_E-set.
    IPR026490. mRNA_cap_0/1_MeTrfase.
    IPR027417. P-loop_NTPase.
    IPR000208. RNA-dir_pol_flavivirus.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR002877. rRNA_MeTrfase_FtsJ_dom.
    IPR029063. SAM-dependent_MTases-like.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF01003. Flavi_capsid. 1 hit.
    PF07652. Flavi_DEAD. 1 hit.
    PF02832. Flavi_glycop_C. 1 hit.
    PF00869. Flavi_glycoprot. 1 hit.
    PF01004. Flavi_M. 1 hit.
    PF00948. Flavi_NS1. 1 hit.
    PF01005. Flavi_NS2A. 1 hit.
    PF01002. Flavi_NS2B. 1 hit.
    PF01350. Flavi_NS4A. 1 hit.
    PF01349. Flavi_NS4B. 1 hit.
    PF00972. Flavi_NS5. 1 hit.
    PF01570. Flavi_propep. 1 hit.
    PF01728. FtsJ. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF00949. Peptidase_S7. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003817. Gen_Poly_FLV. 1 hit.
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF53335. SSF53335. 1 hit.
    SSF56983. SSF56983. 1 hit.
    SSF81296. SSF81296. 1 hit.
    TIGRFAMsiTIGR04240. flavi_E_stem. 1 hit.
    PROSITEiPS51527. FLAVIVIRUS_NS2B. 1 hit.
    PS51528. FLAVIVIRUS_NS3PRO. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51591. RNA_CAP01_NS5_MT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q20IJ2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNQRKKAKN APFNMLKRER NRVSTVQQLT KRFSLGMLQG RGPLKLFMAL     50
    VAFLRFLTIP PTAGILKRWG TIKKSKAINV LRGFRKEIGR MLNILNRRRR 100
    SAGMIIMLIP TVMAFHLTTR NGEPHMIVSM QEKGKSLLFK TEDGVNMCTL 150
    MAMDLGELCE DTITYKCPLL RQNEPEDIDC WCNSTSTWVT YGTCTTTGEH 200
    RREKRSVALV PHVGMGLETR TETWMSSEGA WKHAQRIETW ILRHPGFTIM 250
    AAILAYTIGT THFQRALIFI LLTAVAPSMT MRCIGISNRD FVEGVSGGSW 300
    VDIVLEHGSC VTTMAKNKPT LDFELIKTEA KQPATLRKYC IEAKLTNTTT 350
    ESRCPTQGEP SLKEEQDKRF VCKHSMVDRG WGNGCGLFGK GGIVTCAMFT 400
    CKKNMEGKIV QPENLEYTIV VTPHSGEEHA VGNDTGKHGK EIKVTPQSSI 450
    TEAELTGYGT VTMECSPRTG LDFNEMVLLQ MENKAWLVHR QWFLDLPLPW 500
    LPGADTQGSN WIQKETLVTF KNPHAKKQDV VVLGSQEGAM HTALTGATEI 550
    QMSSGNLLFT GHLKCRLRMD KLQLKGMSYS MCTGKFKVVK EIAETQHGTI 600
    VIRVQYEGDG SPCKIPFEIM DLEKRYVLGR LITVNPIVTE KDSPVNIEAE 650
    PPFGDSYIII GVEPGQLKLN WFKKGSSIGQ MFETTMRGAK RMAILGDTAW 700
    DFGSLGGVFT SIGKALHQVF GAIYGAAFSG VSWTMKILIG VIITWIGMNS 750
    RSTSLSVSLV LVGIVTLYLG VMVQADSGCV VSWKNKELKC GSGIFITDNV 800
    HTWTEQYKFQ PESPSKLASA IQKAQEEGIC GIRSVTRLEN LMWKQITPEL 850
    NHILAENEVK LTIMTGDIKG IMQAGKRSLR PQPTELKYSW KTWGKAKMLS 900
    TESHNQTFLI DGPETAECPN TNRAWNSLEV EDYGFGVFTT NIWLKLKEKQ 950
    DAFCDSKLMS AAIKDNRAVH ADMGYWIESA LNDTWKIEKA SFIEVKNCHW 1000
    PKSHTLWSNG VLESEMIIPK NLAGPVSQHN YRPGYHTQIA GPWHLGKLEM 1050
    DFDFCDGTTV VVTEDCGNRG PSLRTTTASG KLITEWCCRS CTLPPLRYRG 1100
    EDGCWYGMEI RPLKEKEENL VNSLVTAGHG QVDNFSLGVL GMALFLEEML 1150
    RTRVGTKHAI LLVAVSFVTL ITGNMSFKDL GRVVVMVGAT MTDDIGMGVT 1200
    YLALLAAFKV RPTFAAGLLL RKLTSKELMM TTIGIVLLSQ STIPETILEL 1250
    TDALALGMMV LKIVRKMEKY QLAVTIMAIL CVPNAVILQN AWKVSCTILA 1300
    AVSVSPLLLT SSQQKTDWIP LALTIKGLNP TAIFLTTLSR TNKKRSWPLN 1350
    EAIMAVGMVS ILASSLLKND IPMTGPLVAG GLLTVCYVLT GRSADLELER 1400
    ATDVKWEDQA EISGSSPILS ITISEDGSMS IKNEEEEQTL TILIRTGLLV 1450
    ISGLFPVSIP ITAAAWYLWE VKKQRAGVLW DVPSPPPMGK AELEDGAYRI 1500
    KQKGILGYSQ IGAGVYKEGT FHTMWHVTRG AVLMHKGKRI EPSWADVKKD 1550
    LISYGGGWKL EGEWKEGEEV QVLALEPGKN PRAVQTKPGL FKTNTGTIGA 1600
    VSLDFSPGTS GSPIIDKKGK VVGLYGNGVV TRSGAYVSAI AQTEKSIEDN 1650
    PEIEDDIFRK RRLTIMDLHP GAGKTKRYLP AIVREAIKRG LRTLILAPTR 1700
    VVAAEMEEAL RGLPIRYQTP AIRAEHTGRE IVDLMCHATF TMRLLSPVRV 1750
    PNYNLIIMDE AHFTDPASIA ARGYISTRVE MGEAAGIFMT ATPPGSRDPF 1800
    PQSNAPIIDE EREIPERSWN SGHEWVTDFK GKTVWFVPSI KAGNDIAACL 1850
    RKNGKKVIQL SRKTFDSEYV KTRTNDWDFV VTTDISEMGA NFKAERVIDP 1900
    RRCMKPVILT DGEERVILAG PMPVTHSSAA QRRGRIGRNP KNENDQYIYM 1950
    GEPLENDEDC AHWKEAKMLL DNINTPEGII PSMFEPEREK VDAIDGEYRL 2000
    RGEARKTFVD LMRRGDLPVW LAYKVAAEGI NYADRRWCFD GIKNNQILEE 2050
    NVEVEIWTKE GERKKLKPRW LDARIYSDPL ALKEFKEFAA GRKSLALNLI 2100
    TEMGRLPTFM TQKTRDALDN LAVLHTAEAG GRAYNHALSE LPETLETLLL 2150
    LTLLATVTGG IFLFLMSGRG IGKMTLGMCC IITASILLWY AQIQPHWIAA 2200
    SIILEFFLIV LLIPEPEKQR TPQDNQLTYV VIAILTVVAA TMANEMGFLE 2250
    KTKKDLGLGS IATHQPESNI LDIDLRPASA WTLYAVATTF VTPMLRHSIE 2300
    NSSVNVSLTA IANQATVLMG LGKGWPLSKM DIGVPLLAIG CYSQVNPITL 2350
    TAALLLLVAH YAIIGPGLQA KATREAQKRA AAGIMKNPTV DGITVIDLDP 2400
    IPYDPKFEKQ LGQVMLLVLC VTQVLMMRTT WALCEALTLA TGPISTLWEG 2450
    NPGRFWNTTI AVSMANIFRG SYLAGAGLLF SIMKNTTNTR RGTGNIGETL 2500
    GEKWKSRLNA LGKSEFQIYK KSGIQEVDRT LAKEGIKRGE TDHHAVSRGS 2550
    AKLRWFVERN MVTPEGKVVD LGCGRGGWSY YCGGLKNVRE VKGLTKGGPG 2600
    HEEPIPMSTY GWNLVRLQSG VDVFFIPPEK CDTLLCDIGE SSPNPTVEAG 2650
    RTLRVLNLVE NWLNNNTQFC IKVLNPYMPS VIEKMEALQR KYGGALVRNP 2700
    LSRNSTHEMY WVSNASGNIV SSVNMISRML INRFTMRHKK ATYEPDVDLG 2750
    SGTRNIGIES EIPNLDIIGK RIEKIKQEHE TSWHYDQDHP YKTWAYHGSY 2800
    ETKQTGSASS MVNGVVRLLT KPWDVLPTVT QMAMTDTTPF GQQRVFKEKV 2850
    DTRTQEPKEG TKKLMKITAE WLWKELGKKK TPRMCTREEF TRKVRSNAAL 2900
    GAIFTDENKW KSAREAVEDS RFWELVDKER NLHLEGKCET CVYNMMGKRE 2950
    KKLGEFGKAK GSRAIWYMWL GARFLEFEAL GFLNEDHWFS RENSLSGVEG 3000
    EGLHKLGYIL RDVSKKEGGA MYADDTAGWD TRITLEDLKN EEMVTNHMEG 3050
    EHKKLAEAIF RLTYQNKVVR VQRPTPRGTV MDIISRRDQR GSGQVGTYGL 3100
    NTFTNMEAQL IRQMEGEGVF KNIQHLTVTE EIAVQNWLAR VGRERLSRMA 3150
    ISGDDCVVKP LDDRFASALT ALNDMGKIRK DIQQWEPSRG WNDWTQVPFC 3200
    SHHFHELIMK DGRVLVVPCR NQDELIGRAR ISQGAGWSLR ETACLGKSYA 3250
    QMWSLMYFHR RDLRLAANAI CSAVPSHWVP TSRTTWSIHA KHEWMTTEDM 3300
    LTVWNRVWIQ ENPWMEDKTP VESWEEIPYL GKREDQWCGS LIGLTSRATW 3350
    AKNIQAAINQ VRSLIGNEEY TDYMPSMKRF RREEEEAGVL W 3391
    Length:3,391
    Mass (Da):379,453
    Last modified:April 18, 2006 - v1
    Checksum:iE78A50ADF58D2EA5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ181799 Genomic RNA. Translation: ABA61178.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ181799 Genomic RNA. Translation: ABA61178.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2P3Q X-ray 2.75 A 2495-2787 [» ]
    ProteinModelPortali Q20IJ2.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q20IJ2.

    Family and domain databases

    Gene3Di 2.60.40.350. 1 hit.
    2.60.98.10. 2 hits.
    3.30.387.10. 1 hit.
    3.40.50.150. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR011492. DEAD_Flavivir.
    IPR000069. Env_glycoprot_M_flavivir.
    IPR013755. Flav_gly_cen_dom_subdom1.
    IPR001122. Flavi_capsidC.
    IPR026470. Flavi_E_Stem/Anchor_dom.
    IPR001157. Flavi_NS1.
    IPR000752. Flavi_NS2A.
    IPR000487. Flavi_NS2B.
    IPR000404. Flavi_NS4A.
    IPR001528. Flavi_NS4B.
    IPR002535. Flavi_propep.
    IPR000336. Flavivir/Alphavir_Ig-like.
    IPR001850. Flavivirus_NS3_S7.
    IPR027287. Flavovir_Ig-like.
    IPR014412. Gen_Poly_FLV.
    IPR011998. Glycoprot_cen/dimer.
    IPR013754. GlyE_dim.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR014756. Ig_E-set.
    IPR026490. mRNA_cap_0/1_MeTrfase.
    IPR027417. P-loop_NTPase.
    IPR000208. RNA-dir_pol_flavivirus.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR002877. rRNA_MeTrfase_FtsJ_dom.
    IPR029063. SAM-dependent_MTases-like.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF01003. Flavi_capsid. 1 hit.
    PF07652. Flavi_DEAD. 1 hit.
    PF02832. Flavi_glycop_C. 1 hit.
    PF00869. Flavi_glycoprot. 1 hit.
    PF01004. Flavi_M. 1 hit.
    PF00948. Flavi_NS1. 1 hit.
    PF01005. Flavi_NS2A. 1 hit.
    PF01002. Flavi_NS2B. 1 hit.
    PF01350. Flavi_NS4A. 1 hit.
    PF01349. Flavi_NS4B. 1 hit.
    PF00972. Flavi_NS5. 1 hit.
    PF01570. Flavi_propep. 1 hit.
    PF01728. FtsJ. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF00949. Peptidase_S7. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003817. Gen_Poly_FLV. 1 hit.
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF53335. SSF53335. 1 hit.
    SSF56983. SSF56983. 1 hit.
    SSF81296. SSF81296. 1 hit.
    TIGRFAMsi TIGR04240. flavi_E_stem. 1 hit.
    PROSITEi PS51527. FLAVIVIRUS_NS2B. 1 hit.
    PS51528. FLAVIVIRUS_NS3PRO. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51591. RNA_CAP01_NS5_MT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE.
      Strain: ThD2_0017_98Imported.
    2. "Structural and functional analysis of methylation and 5'-RNA sequence requirements of short capped RNAs by the methyltransferase domain of dengue virus NS5."
      Egloff M.P., Decroly E., Malet H., Selisko B., Benarroch D., Ferron F., Canard B.
      J. Mol. Biol. 372:723-736(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS) OF 2495-2787 IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE.

    Entry informationi

    Entry nameiQ20IJ2_9FLAV
    AccessioniPrimary (citable) accession number: Q20IJ2
    Entry historyi
    Integrated into UniProtKB/TrEMBL: April 18, 2006
    Last sequence update: April 18, 2006
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3