ID   SYMC_CAEEL              Reviewed;         917 AA.
AC   Q20970;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 68.
DE   RecName: Full=Methionyl-tRNA synthetase, cytoplasmic;
DE            EC=6.1.1.10;
DE   AltName: Full=Methionine--tRNA ligase;
DE            Short=MetRS;
GN   Name=mrs-1; ORFNames=F58B3.5;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Nematoda; Chromadorea; Rhabditida; Rhabditoidea;
OC   Rhabditidae; Peloderinae; Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   MEDLINE=99069613; PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for
RT   investigating biology.";
RL   Science 282:2012-2018(1998).
CC   -!- CATALYTIC ACTIVITY: ATP + L-methionine + tRNA(Met) = AMP +
CC       diphosphate + L-methionyl-tRNA(Met).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase
CC       family.
CC   -!- SIMILARITY: Contains 1 tRNA-binding domain.
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DR   EMBL; Z73427; CAA97803.1; -; Genomic_DNA.
DR   PIR; T22898; T22898.
DR   RefSeq; NP_502196.1; -.
DR   UniGene; Cel.16977; -.
DR   HSSP; Q12904; 1FL0.
DR   Ensembl; F58B3.5; Caenorhabditis elegans.
DR   GeneID; 178089; -.
DR   KEGG; cel:F58B3.5; -.
DR   NMPDR; fig|6239.3.peg.15257; -.
DR   WormBase; WBGene00003415; mrs-1.
DR   WormPep; F58B3.5; CE06007.
DR   OMA; Q20970; LVSHEIA.
DR   BRENDA; 6.1.1.10; 672.
DR   NextBio; 899666; -.
DR   ArrayExpress; Q20970; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004825; F:methionine-tRNA ligase activity; IEA:EC.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009792; P:embryonic development ending in birth or eg...; IMP:WormBase.
DR   GO; GO:0040007; P:growth; IMP:WormBase.
DR   GO; GO:0006431; P:methionyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0002119; P:nematode larval development; IMP:WormBase.
DR   GO; GO:0040010; P:positive regulation of growth rate; IMP:WormBase.
DR   GO; GO:0000003; P:reproduction; IMP:WormBase.
DR   InterPro; IPR015413; aa-tRNA-synt_I.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002304; Met-tRNA-synth_Ia.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR014758; tRNA-synt_met_N.
DR   InterPro; IPR002547; tRNA_bd.
DR   Gene3D; G3DSA:2.40.50.140; OB_NA_bd_sub; 1.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   Pfam; PF01588; tRNA_bind; 1.
DR   PRINTS; PR01041; TRNASYNTHMET.
DR   TIGRFAMs; TIGR00398; metG; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
DR   PROSITE; PS50886; TRBD; 1.
PE   2: Evidence at transcript level;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm;
KW   Ligase; Nucleotide-binding; Protein biosynthesis; RNA-binding;
KW   tRNA-binding.
FT   CHAIN         1    917       Methionyl-tRNA synthetase, cytoplasmic.
FT                                /FTId=PRO_0000139265.
FT   DOMAIN      756    857       tRNA-binding.
FT   MOTIF        44     54       "HIGH" region.
FT   MOTIF       367    371       "KMSKS" region.
FT   BINDING     370    370       ATP (By similarity).
SQ   SEQUENCE   917 AA;  101714 MW;  3B42AABA314FBDF0 CRC64;
     MGHDLADIKK SFEASLPGYV EKKDPKSILP QPGKRNILIT AALPYVNNVP HLGNIIGCVL
     SADVFARYCN LRGHQTFYVG GTDEYGTATE TKALQEGCTP RELCDKYHAI HKGIYEWFGI
     DFSHFGRTTT DHQTEICQDM FLKLHKNGYT SSQSVDQLYC NQCEKFLADR FVTGTCPMCA
     YDDARGDQCD GCGKLINAVD LKDAKCHMCK ATPEVKQSTH IFLSLDKLQQ KTTEHLDREL
     AKEDNRWSSN AVGITKAWMK LGLDPRCITR DLKWGTAVPL DGFEKKVFYV WFDAPIGYLS
     ITKCVLGDNW TKWWKNPENV ELFNFVGKDN VAFHAVMFPC SQLGANDNYT VVNNLCATEY
     LNYEDTKFSK SRGTGIFGDA AQGTEIPADI WRFYLLYMRP ESQDTAFSWD DFVLKVNSEL
     LNNLGNFINR ALSFVANSFG GVVPEMNLTN DDAEVLSEIH NECMQWDKQF DGVHLKDAVK
     TILNVSRLGN QYMQAQTPWV LMKKDEEGKK RAGTIIGVAA NIAYHVSVLL YPIMPTISAT
     IREQCGLPAL PLFTPFPICY LKAGHKIGQP SPLFQKLDPA QIAEFKAKFG GSQDAQSSAP
     KTAEKPKQQK KQAPTKDKKG DKKMASTAAF VELEQGAKVI SQLIAQNLKK FDQAKALFTR
     NQLQRLDGEN KQLTIDVKTL QHQLIELETA AGIKQVPKPV VSCTPTPTST PASGIITEAP
     KKEAPSTPAP SEPKKAKEQK KGKGGAAAAP VDDTIDVGRL DMRVGRIIKC EKHPDADALY
     VEQIDVGESA PRTVVSGLVR HVPLDQMQNR LVVVLCNLKP AKMRGVESRA MVMCASSPDK
     VEIMEVPADS KPGTPVVCPP YTHRPDEQLN PKKKIWETVA EDLKVSAEGF AEWKGQPLLI
     GSESKMTAPT LRGVHVK
//