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Protein

Methionine--tRNA ligase, cytoplasmic

Gene

mrs-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei370 – 3701ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase, cytoplasmic (EC:6.1.1.10)
Alternative name(s):
Methionyl-tRNA synthetase
Short name:
MetRS
Gene namesi
Name:mrs-1
ORF Names:F58B3.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiF58B3.5a; CE06007; WBGene00003415; mars-1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 917917Methionine--tRNA ligase, cytoplasmicPRO_0000139265Add
BLAST

Proteomic databases

EPDiQ20970.
PaxDbiQ20970.
PRIDEiQ20970.

PTM databases

iPTMnetiQ20970.

Expressioni

Gene expression databases

ExpressionAtlasiQ20970. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi6239.F58B3.5a.

Structurei

3D structure databases

ProteinModelPortaliQ20970.
SMRiQ20970. Positions 38-622, 755-917.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini756 – 857102tRNA-bindingAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi44 – 5411"HIGH" regionAdd
BLAST
Motifi367 – 3715"KMSKS" region

Sequence similaritiesi

Contains 1 tRNA-binding domain.Curated

Phylogenomic databases

eggNOGiKOG1247. Eukaryota.
KOG2241. Eukaryota.
COG0073. LUCA.
COG0143. LUCA.
GeneTreeiENSGT00550000075017.
HOGENOMiHOG000200402.
InParanoidiQ20970.
KOiK01874.
OMAiHAIHKGI.
OrthoDBiEOG7P8P74.
PhylomeDBiQ20970.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.20.28.20. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR029038. MetRS_Zn.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q20970-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGHDLADIKK SFEASLPGYV EKKDPKSILP QPGKRNILIT AALPYVNNVP
60 70 80 90 100
HLGNIIGCVL SADVFARYCN LRGHQTFYVG GTDEYGTATE TKALQEGCTP
110 120 130 140 150
RELCDKYHAI HKGIYEWFGI DFSHFGRTTT DHQTEICQDM FLKLHKNGYT
160 170 180 190 200
SSQSVDQLYC NQCEKFLADR FVTGTCPMCA YDDARGDQCD GCGKLINAVD
210 220 230 240 250
LKDAKCHMCK ATPEVKQSTH IFLSLDKLQQ KTTEHLDREL AKEDNRWSSN
260 270 280 290 300
AVGITKAWMK LGLDPRCITR DLKWGTAVPL DGFEKKVFYV WFDAPIGYLS
310 320 330 340 350
ITKCVLGDNW TKWWKNPENV ELFNFVGKDN VAFHAVMFPC SQLGANDNYT
360 370 380 390 400
VVNNLCATEY LNYEDTKFSK SRGTGIFGDA AQGTEIPADI WRFYLLYMRP
410 420 430 440 450
ESQDTAFSWD DFVLKVNSEL LNNLGNFINR ALSFVANSFG GVVPEMNLTN
460 470 480 490 500
DDAEVLSEIH NECMQWDKQF DGVHLKDAVK TILNVSRLGN QYMQAQTPWV
510 520 530 540 550
LMKKDEEGKK RAGTIIGVAA NIAYHVSVLL YPIMPTISAT IREQCGLPAL
560 570 580 590 600
PLFTPFPICY LKAGHKIGQP SPLFQKLDPA QIAEFKAKFG GSQDAQSSAP
610 620 630 640 650
KTAEKPKQQK KQAPTKDKKG DKKMASTAAF VELEQGAKVI SQLIAQNLKK
660 670 680 690 700
FDQAKALFTR NQLQRLDGEN KQLTIDVKTL QHQLIELETA AGIKQVPKPV
710 720 730 740 750
VSCTPTPTST PASGIITEAP KKEAPSTPAP SEPKKAKEQK KGKGGAAAAP
760 770 780 790 800
VDDTIDVGRL DMRVGRIIKC EKHPDADALY VEQIDVGESA PRTVVSGLVR
810 820 830 840 850
HVPLDQMQNR LVVVLCNLKP AKMRGVESRA MVMCASSPDK VEIMEVPADS
860 870 880 890 900
KPGTPVVCPP YTHRPDEQLN PKKKIWETVA EDLKVSAEGF AEWKGQPLLI
910
GSESKMTAPT LRGVHVK
Length:917
Mass (Da):101,714
Last modified:November 1, 1996 - v1
Checksum:i3B42AABA314FBDF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73427 Genomic DNA. Translation: CAA97803.1.
PIRiT22898.
RefSeqiNP_001255570.1. NM_001268641.1.
UniGeneiCel.16977.
Cel.40250.

Genome annotation databases

EnsemblMetazoaiF58B3.5a; F58B3.5a; WBGene00003415.
GeneIDi178089.
KEGGicel:CELE_F58B3.5.
UCSCiF58B3.5. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73427 Genomic DNA. Translation: CAA97803.1.
PIRiT22898.
RefSeqiNP_001255570.1. NM_001268641.1.
UniGeneiCel.16977.
Cel.40250.

3D structure databases

ProteinModelPortaliQ20970.
SMRiQ20970. Positions 38-622, 755-917.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.F58B3.5a.

PTM databases

iPTMnetiQ20970.

Proteomic databases

EPDiQ20970.
PaxDbiQ20970.
PRIDEiQ20970.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF58B3.5a; F58B3.5a; WBGene00003415.
GeneIDi178089.
KEGGicel:CELE_F58B3.5.
UCSCiF58B3.5. c. elegans.

Organism-specific databases

CTDi178089.
WormBaseiF58B3.5a; CE06007; WBGene00003415; mars-1.

Phylogenomic databases

eggNOGiKOG1247. Eukaryota.
KOG2241. Eukaryota.
COG0073. LUCA.
COG0143. LUCA.
GeneTreeiENSGT00550000075017.
HOGENOMiHOG000200402.
InParanoidiQ20970.
KOiK01874.
OMAiHAIHKGI.
OrthoDBiEOG7P8P74.
PhylomeDBiQ20970.

Miscellaneous databases

NextBioi899666.
PROiQ20970.

Gene expression databases

ExpressionAtlasiQ20970. baseline and differential.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.20.28.20. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR029038. MetRS_Zn.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiSYMC_CAEEL
AccessioniPrimary (citable) accession number: Q20970
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.