Reviewed, UniProtKB/Swiss-Prot Q20970 (SYMC_CAEEL)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	mrs-1
ORF Names:	F58B3.5

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	917 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro nematode larval development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase reproduction Inferred from mutant phenotype. Source: WormBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 917	917	Methionyl-tRNA synthetase, cytoplasmic		PRO_0000139265
Regions
	Domain	756 – 857	102	tRNA-binding
	Motif	44 – 54	11	"HIGH" region
	Motif	367 – 371	5	"KMSKS" region
Sites
	Binding site	370	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q20970-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3B42AABA314FBDF0	FASTA	917	101,714
	10 20 30 40 50 60 MGHDLADIKK SFEASLPGYV EKKDPKSILP QPGKRNILIT AALPYVNNVP HLGNIIGCVL 70 80 90 100 110 120 SADVFARYCN LRGHQTFYVG GTDEYGTATE TKALQEGCTP RELCDKYHAI HKGIYEWFGI 130 140 150 160 170 180 DFSHFGRTTT DHQTEICQDM FLKLHKNGYT SSQSVDQLYC NQCEKFLADR FVTGTCPMCA 190 200 210 220 230 240 YDDARGDQCD GCGKLINAVD LKDAKCHMCK ATPEVKQSTH IFLSLDKLQQ KTTEHLDREL 250 260 270 280 290 300 AKEDNRWSSN AVGITKAWMK LGLDPRCITR DLKWGTAVPL DGFEKKVFYV WFDAPIGYLS 310 320 330 340 350 360 ITKCVLGDNW TKWWKNPENV ELFNFVGKDN VAFHAVMFPC SQLGANDNYT VVNNLCATEY 370 380 390 400 410 420 LNYEDTKFSK SRGTGIFGDA AQGTEIPADI WRFYLLYMRP ESQDTAFSWD DFVLKVNSEL 430 440 450 460 470 480 LNNLGNFINR ALSFVANSFG GVVPEMNLTN DDAEVLSEIH NECMQWDKQF DGVHLKDAVK 490 500 510 520 530 540 TILNVSRLGN QYMQAQTPWV LMKKDEEGKK RAGTIIGVAA NIAYHVSVLL YPIMPTISAT 550 560 570 580 590 600 IREQCGLPAL PLFTPFPICY LKAGHKIGQP SPLFQKLDPA QIAEFKAKFG GSQDAQSSAP 610 620 630 640 650 660 KTAEKPKQQK KQAPTKDKKG DKKMASTAAF VELEQGAKVI SQLIAQNLKK FDQAKALFTR 670 680 690 700 710 720 NQLQRLDGEN KQLTIDVKTL QHQLIELETA AGIKQVPKPV VSCTPTPTST PASGIITEAP 730 740 750 760 770 780 KKEAPSTPAP SEPKKAKEQK KGKGGAAAAP VDDTIDVGRL DMRVGRIIKC EKHPDADALY 790 800 810 820 830 840 VEQIDVGESA PRTVVSGLVR HVPLDQMQNR LVVVLCNLKP AKMRGVESRA MVMCASSPDK 850 860 870 880 890 900 VEIMEVPADS KPGTPVVCPP YTHRPDEQLN PKKKIWETVA EDLKVSAEGF AEWKGQPLLI 910 GSESKMTAPT LRGVHVK

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z73427 Genomic DNA. Translation: CAA97803.1.
PIR	T22898.
RefSeq	NP_502196.1.
UniGene	Cel.16977
3D structure databases
HSSP	HSSP built from PDB template 1FL0 based on UniProtKB Q12904.
ModBase	Search...
Protein-protein interaction databases
STRING	Q20970.
Genome annotation databases
Ensembl	F58B3.5; F58B3.5; F58B3.5; Caenorhabditis elegans. [Genome view]
GeneID	178089.
KEGG	cel:F58B3.5.
NMPDR	fig|6239.3.peg.15257.
UCSC	F58B3.5. c. elegans.
Organism-specific databases
CTD	178089.
WormBase	WBGene00003415. mrs-1.
WormPep	F58B3.5. CE06007. [WorfDB]
Phylogenomic databases
OMA	LVSHEIA.
Enzyme and pathway databases
BRENDA	6.1.1.10. 672.
Gene expression databases
ArrayExpress	Q20970.
Family and domain databases
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR002547. tRNA_bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	899666.

Entry information

Entry name

SYMC_CAEEL

Accession

Primary (citable) accession number: Q20970

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project