Reviewed, UniProtKB/Swiss-Prot Q20958 (NAS29_CAEEL)

Names and origin

Protein names

Recommended name:
    Zinc metalloproteinase nas-29
    EC=3.4.24.21
Alternative name(s):
    Nematode astacin 29

Gene names

Name:	nas-29
ORF Names:	F58A6.4

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable metalloprotease By similarity.
Catalytic activity	Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the peptidase M12A family. Contains 1 CUB domain. Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	EGF-like domain Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 532	510	Zinc metalloproteinase nas-29		PRO_0000028933
Regions
	Domain	330 – 370	41	EGF-like
	Domain	380 – 494	115	CUB
Sites
	Active site	231	1	By similarity
	Metal binding	230	1	Zinc; catalytic By similarity
	Metal binding	234	1	Zinc; catalytic By similarity
	Metal binding	240	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	5	1	N-linked (GlcNAc...) Potential
	Glycosylation	27	1	N-linked (GlcNAc...) Potential
	Glycosylation	70	1	N-linked (GlcNAc...) Potential
	Glycosylation	106	1	N-linked (GlcNAc...) Potential
	Glycosylation	503	1	N-linked (GlcNAc...) Potential
	Disulfide bond	334 ↔ 344		By similarity
	Disulfide bond	338 ↔ 358		By similarity
	Disulfide bond	360 ↔ 369		By similarity
	Disulfide bond	380 ↔ 408		By similarity
	Disulfide bond	435 ↔ 456		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q20958-1 [UniParc]. Last modified December 16, 2008. Version 4. Checksum: 29FBD634CB685A08	FASTA	532	60,395
	10 20 30 40 50 60 MISKNTSFCG FLILVLATCM SAQFVSNESI KLHDILKPSA THRLFDTLQY SVEEQYSDSH 70 80 90 100 110 120 LSFDVSTIYN YSEKPISIGK LNKKYRDILF EGDMAISYKQ LSMIVNGSTE YRKAIKSRRR 130 140 150 160 170 180 GNKINGESTD RTKRQAYLDN NYPATIWKNG VAFMFHESLT PIAKTAILKA VHFWYRETCI 190 200 210 220 230 240 EFHPRTFQKE YLLFIGNDDG CWSTVGRDAS QGKQVVSIGN GCEHFGVTSH ELAHALGIFH 250 260 270 280 290 300 EQSRFDRDES VVFNPRVVER DLLFNFAKIS PRQMSTYGLP YDIGSVMHYT PTEFSNIPSI 310 320 330 340 350 360 PTLAAIDTNL QQTMGQLEGP SFVDVHIMNQ HYQCQEKCPT QAPCQNGGFT NSRNCKVCKC 370 380 390 400 410 420 PTGFGGAYCQ LIASSFSPFC GGYLNAEETT RRFDITIRQS TTTRSKTCVY HIKAPEGKRI 430 440 450 460 470 480 IIDILKIDSK CIEGCWQDGL ELKMKKDFRP VGYRFCCPES SRRKVISEGN MVPFMVFSKE 490 500 510 520 530 HDFSVSFEYS FVSTSAGFDD EKNDSDVIVD NLDGVFVSDT SLLQRIGFRR QL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"The astacin protein family in Caenorhabditis elegans." Moehrlen F., Hutter H., Zwilling R. Eur. J. Biochem. 270:4909-4920(2003) [PubMed: 14653817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 197-286, GENE STRUCTURE, NOMENCLATURE. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U53339 Genomic DNA. Translation: AAA96198.3. AJ561218 mRNA. Translation: CAD99218.1.
PIR	T16493.
RefSeq	NP_494953.3.
UniGene	Cel.14942
3D structure databases
HSSP	HSSP built from PDB template 1IAE based on UniProtKB P07584.
ModBase	Search...
Protein family/group databases
MEROPS	M12.310.
Genome annotation databases
Ensembl	F58A6.4. Caenorhabditis elegans. [Contig view]
GeneID	186488.
KEGG	cel:F58A6.4.
Organism-specific databases
WormBase	WBGene00003547. nas-29.
WormPep	F58A6.4. CE42691. [WorfDB]
Enzyme and pathway databases
BRENDA	3.4.24.21. 672.
Gene expression databases
ArrayExpress	Q20958.
Family and domain databases
InterPro	IPR000859. CUB. IPR013032. EGF-like_reg_CS. IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR001506. Peptidase_M12A. [Graphical view]
Gene3D	G3DSA:2.60.120.290. CUB. 1 hit.
Pfam	PF01400. Astacin. 1 hit. PF00431. CUB. 1 hit. [Graphical view]
PRINTS	PR00480. ASTACIN.
SMART	SM00042. CUB. 1 hit. SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS01180. CUB. 1 hit. PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	932008.

Entry information

Entry name

NAS29_CAEEL

Accession

Primary (citable) accession number: Q20958
Secondary accession number(s): Q7Z0M3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	December 16, 2008
Last modified:	June 16, 2009
This is version 59 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project