Reviewed, UniProtKB/Swiss-Prot Q20939 (PDXH_CAEEL)

Names and origin

Protein names

Recommended name:
    Putative pyridoxamine 5'-phosphate oxidase
    EC=1.4.3.5
Alternative name(s):
    PNP/PMP oxidase
      Short name=PNPOx

Gene names

ORF Names:

F57B9.1

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP) By similarity.
Catalytic activity	Pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2. Pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2.
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1. Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the pyridoxamine 5'-phosphate oxidase family.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro pyridoxamine-phosphate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Putative pyridoxamine 5'-phosphate oxidase		PRO_0000167788
Regions
	Nucleotide binding	114 – 115	2	FMN By similarity
	Nucleotide binding	178 – 179	2	FMN By similarity
	Region	232 – 234	3	Substrate binding By similarity
Sites
	Binding site	99	1	FMN By similarity
	Binding site	102	1	FMN; via amide nitrogen By similarity
	Binding site	104	1	Substrate By similarity
	Binding site	121	1	FMN By similarity
	Binding site	161	1	Substrate By similarity
	Binding site	165	1	Substrate By similarity
	Binding site	169	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q20939-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 887A80EC98B84958	FASTA	253	29,402
	10 20 30 40 50 60 MFVVPRRLSS FLSLVHEYRH LARRSYVMET PSIDIQNIRA KYLNSHDPYL LESKLPTTSP 70 80 90 100 110 120 FELFDIWFRN VASQSDLTFE EINAVSLSTV GKDLRPSSRM VLLKAYTPTG FSFYTNYTSR 130 140 150 160 170 180 KGNQLEENPN AAMLFYWPKV NRQIRVEGVV EKLPDEMAVA YWNSRPVASR IGSKSSDQSK 190 200 210 220 230 240 VVPDREFLES KKVALTELSV REGAQAITKP ESWGGYHLIP RYFEFWQGQS DRLHDRIVFE 250 RDVDVWLLKR LSP

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U13876 Genomic DNA. Translation: AAA21167.1.
PIR	A88494.
RefSeq	NP_498518.1.
UniGene	Cel.10391
3D structure databases
HSSP	HSSP built from PDB template 1NRG based on UniProtKB Q9NVS9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q20939. 1 interaction.
Genome annotation databases
Ensembl	F57B9.1. Caenorhabditis elegans. [Contig view]
GeneID	175973.
KEGG	cel:F57B9.1.
NMPDR	fig|6239.3.peg.10352.
Organism-specific databases
WormBase	WBGene00018996. F57B9.1.
WormPep	F57B9.1. CE01335. [WorfDB]
Phylogenomic databases
OMA	Q20939. RKGSELE.
Enzyme and pathway databases
BRENDA	1.4.3.5. 672.
Gene expression databases
ArrayExpress	Q20939.
Family and domain databases
InterPro	IPR011576. PNPOx_rel_FMN_bd_core. IPR000659. Pyridoxamine_oxidase. IPR019740. Pyridoxamine_oxidase_CS. IPR019576. Pyridoxamine_oxidase_dimer_C. IPR012349. Split_barrel_FMN_bd. [Graphical view]
Gene3D	G3DSA:2.30.110.10. PNPOx_FMN_bd. 1 hit.
PANTHER	PTHR10851. Pyridox_oxidase. 1 hit.
Pfam	PF10590. PNPOx_C. 1 hit. PF01243. Pyridox_oxidase. 1 hit. [Graphical view]
ProDom	PD006312. Pyridox_oxidase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00558. pdxH. 1 hit.
PROSITE	PS01064. PYRIDOX_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	890562.

Entry information

Entry name

PDXH_CAEEL

Accession

Primary (citable) accession number: Q20939

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project