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Protein

Eukaryotic translation initiation factor 5A-2

Gene

iff-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis (By similarity). Acts in somatic tissues and its function in the soma is essential for normal growth and reproduction.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • gamete generation Source: WormBase
  • gonad morphogenesis Source: WormBase
  • nematode larval development Source: WormBase
  • nematode male tail tip morphogenesis Source: WormBase
  • positive regulation of translational elongation Source: InterPro
  • positive regulation of translational termination Source: InterPro
  • translational frameshifting Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiREACT_335543. Hypusine synthesis from eIF5A-lysine.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5A-2
Short name:
eIF-5A-2
Alternative name(s):
Initiation factor five protein 2
Gene namesi
Name:iff-2
ORF Names:F54C9.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiF54C9.1; CE02249; WBGene00002065; iff-2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 161161Eukaryotic translation initiation factor 5A-2PRO_0000142457Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei54 – 541HypusineBy similarity

Post-translational modificationi

eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).

Keywords - PTMi

Hypusine

Proteomic databases

PaxDbiQ20751.
PRIDEiQ20751.

Expressioni

Tissue specificityi

Expressed in the somatic tissues.1 Publication

Developmental stagei

Expressed throughout development.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi6239.F54C9.1.3.

Structurei

3D structure databases

ProteinModelPortaliQ20751.
SMRiQ20751. Positions 15-160.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eIF-5A family.Curated

Phylogenomic databases

eggNOGiCOG0231.
GeneTreeiENSGT00390000003738.
HOGENOMiHOG000106270.
InParanoidiQ20751.
KOiK03263.
OMAiKEYEDIC.
PhylomeDBiQ20751.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
InterProiIPR005824. KOW.
IPR012340. NA-bd_OB-fold.
IPR014722. Rib_L2_dom2.
IPR019769. Trans_elong_IF5A_hypusine_site.
IPR001884. Transl_elong_IF5A.
IPR020189. Transl_elong_IF5A_C.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERiPTHR11673. PTHR11673. 1 hit.
PfamiPF01287. eIF-5a. 1 hit.
PF00467. KOW. 1 hit.
[Graphical view]
PIRSFiPIRSF003025. eIF5A. 1 hit.
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00037. eIF_5A. 1 hit.
PROSITEiPS00302. IF5A_HYPUSINE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q20751-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDDHHDDEH FHTGDSGAAA TFPKQCSALR KNEHVMIKGR PCKIVEMSTS
60 70 80 90 100
KTGKHGHAKV HMVAIDIFTS KKLEDICPST HNMDVPVVKR REYLLMAIDD
110 120 130 140 150
GYCSLMDPES CEQKDDLKLP DTELGQQIRD AYEKDEGSVL VQVVSAIGEE
160
AILGWKVSTK E
Length:161
Mass (Da):17,953
Last modified:November 1, 1997 - v1
Checksum:iE497AFDE6580E53B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49967 Genomic DNA. Translation: CAA90247.1.
PIRiT22628.
RefSeqiNP_495807.1. NM_063406.6.
UniGeneiCel.22474.

Genome annotation databases

EnsemblMetazoaiF54C9.1.1; F54C9.1.1; WBGene00002065.
F54C9.1.2; F54C9.1.2; WBGene00002065.
GeneIDi174367.
KEGGicel:CELE_F54C9.1.
UCSCiF54C9.1.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49967 Genomic DNA. Translation: CAA90247.1.
PIRiT22628.
RefSeqiNP_495807.1. NM_063406.6.
UniGeneiCel.22474.

3D structure databases

ProteinModelPortaliQ20751.
SMRiQ20751. Positions 15-160.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.F54C9.1.3.

Proteomic databases

PaxDbiQ20751.
PRIDEiQ20751.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF54C9.1.1; F54C9.1.1; WBGene00002065.
F54C9.1.2; F54C9.1.2; WBGene00002065.
GeneIDi174367.
KEGGicel:CELE_F54C9.1.
UCSCiF54C9.1.1. c. elegans.

Organism-specific databases

CTDi174367.
WormBaseiF54C9.1; CE02249; WBGene00002065; iff-2.

Phylogenomic databases

eggNOGiCOG0231.
GeneTreeiENSGT00390000003738.
HOGENOMiHOG000106270.
InParanoidiQ20751.
KOiK03263.
OMAiKEYEDIC.
PhylomeDBiQ20751.

Enzyme and pathway databases

ReactomeiREACT_335543. Hypusine synthesis from eIF5A-lysine.

Miscellaneous databases

NextBioi883730.
PROiQ20751.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
InterProiIPR005824. KOW.
IPR012340. NA-bd_OB-fold.
IPR014722. Rib_L2_dom2.
IPR019769. Trans_elong_IF5A_hypusine_site.
IPR001884. Transl_elong_IF5A.
IPR020189. Transl_elong_IF5A_C.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERiPTHR11673. PTHR11673. 1 hit.
PfamiPF01287. eIF-5a. 1 hit.
PF00467. KOW. 1 hit.
[Graphical view]
PIRSFiPIRSF003025. eIF5A. 1 hit.
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00037. eIF_5A. 1 hit.
PROSITEiPS00302. IF5A_HYPUSINE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "The Caenorhabditis elegans eukaryotic initiation factor 5A homologue, IFF-1, is required for germ cell proliferation, gametogenesis and localization of the P-granule component PGL-1."
    Hanazawa M., Kawasaki I., Kunitomo H., Gengyo-Ando K., Bennett K.L., Mitani S., Iino Y.
    Mech. Dev. 121:213-224(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiIF5A2_CAEEL
AccessioniPrimary (citable) accession number: Q20751
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 22, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Translation initiation factors
    List of translation initiation factor entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.