Reviewed, UniProtKB/Swiss-Prot Q20728 (TBCB_CAEEL)

Names and origin

Protein names

Recommended name:
    Tubulin-specific chaperone B
Alternative name(s):
    Tubulin folding cofactor B
      Short name=CoB

Gene names

ORF Names:

F53F4.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer By similarity.
Subunit structure	Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state By similarity.
Subcellular location	CytoplasmBy similarity. Cytoplasm › cytoskeletonBy similarity.
Sequence similarities	Belongs to the TBCB family. Contains 1 CAP-Gly domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Molecular function	Chaperone
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 229

229

Tubulin-specific chaperone B

Regions

Domain

170 – 212

43

CAP-Gly

Secondary structure

1

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229

Helix Strand Turn

Details...

Beta strand

4 – 13

10

Beta strand

18 – 23

6

Helix

28 – 39

12

Turn

43 – 45

3

Beta strand

46 – 51

6

Beta strand

53 – 60

8

Beta strand

65 – 67

3

Turn

68 – 72

5

Beta strand

77 – 83

7

Helix

137 – 143

7

Beta strand

151 – 154

4

Beta strand

162 – 170

9

Beta strand

173 – 177

5

Beta strand

179 – 187

9

Beta strand

189 – 195

7

Beta strand

207 – 211

5

Helix

213 – 215

3

Beta strand

216 – 219

4

Sequences

	Sequence		Length	Mass (Da)
	Q20728-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C465365DAE378A0F	FASTA	229	25,441
	10 20 30 40 50 60 MTEVYDLEIT TNATDFPMEK KYPAGMSLND LKKKLELVVG TTVDSMRIQL FDGDDQLKGE 70 80 90 100 110 120 LTDGAKSLKD LGVRDGYRIH AVDVTGGNED FKDESMVEKY EMSDDTYGKR TDSVRAWKKK 130 140 150 160 170 180 MQEEQGSAAP MENESDKLNE EAAKNIMVGN RCEVTVGAQM ARRGEVAYVG ATKFKEGVWV 190 200 210 220 GVKYDEPVGK NDGSVAGVRY FDCDPKYGGF VRPVDVKVGD FPELSIDEI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Crystal structure of the cytoskeleton-associated protein glycine-rich (CAP-Gly) domain." Li S., Finley J., Liu Z.J., Qiu S.H., Chen H., Luan C.H., Carson M., Tsao J., Johnson D., Lin G., Zhao J., Thomas W., Nagy L.A., Sha B., DeLucas L.J., Wang B.C., Luo M. J. Biol. Chem. 277:48596-48601(2002) [PubMed: 12221106] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.77 ANGSTROMS) OF 135-229.
[3]	"Solution structure of a ubiquitin-like domain from tubulin-binding cofactor B." Lytle B.L., Peterson F.C., Qiu S.H., Luo M., Zhao Q., Markley J.L., Volkman B.F. J. Biol. Chem. 279:46787-46793(2004) [PubMed: 15364906] [Abstract] Cited for: STRUCTURE BY NMR OF 1-120.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Z77663 Genomic DNA. Translation: CAB01212.1.

PIR

T22581.

RefSeq

NP_506367.1.

UniGene

Cel.3444

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LPL	X-ray	1.77	A	135-229	[»]
1T0Y	NMR	-	A	1-120	[»]
1TOV	X-ray	1.77	A	132-229	[»]

ModBase

Search...

Genome annotation databases

Ensembl

F53F4.3. Caenorhabditis elegans. [Contig view]

GeneID

186176.

KEGG

cel:F53F4.3.

NMPDR

fig|6239.3.peg.20325.

Organism-specific databases

WormBase

WBGene00009987. F53F4.3.

WormPep

F53F4.3. CE10958. [WorfDB]

Gene expression databases

ArrayExpress

Q20728.

Family and domain databases

InterPro

IPR000938. Cytoskel-assoc-prot_CAP-Gly.
[Graphical view]

Pfam

PF01302. CAP_GLY. 1 hit.
[Graphical view]

PROSITE

PS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.
[Graphical view]

ProDom

Q20728.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

ProtoNet

Search...

Entry information

Entry name

TBCB_CAEEL

Accession

Primary (citable) accession number: Q20728

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	July 22, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project