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Protein

Probable NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

Gene

F53F4.10

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[2Fe-2S] clusterCuratedNote: Binds 1 [2Fe-2S] cluster.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi124 – 1241Iron-sulfur (2Fe-2S)Sequence Analysis
Metal bindingi129 – 1291Iron-sulfur (2Fe-2S)Sequence Analysis
Metal bindingi165 – 1651Iron-sulfur (2Fe-2S)Sequence Analysis
Metal bindingi169 – 1691Iron-sulfur (2Fe-2S)Sequence Analysis

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
Probable NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Gene namesi
ORF Names:F53F4.10
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiF53F4.10; CE10972; WBGene00009992.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 239Probable NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrialPRO_0000020006
Transit peptidei1 – ?MitochondrionSequence Analysis

Proteomic databases

PaxDbiQ20719.

2D gel databases

World-2DPAGE0020:Q20719.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme (By similarity).By similarity

Protein-protein interaction databases

BioGridi44866. 1 interaction.
DIPiDIP-26781N.
IntActiQ20719. 1 interaction.
MINTiMINT-1083480.
STRINGi6239.F53F4.10.2.

Structurei

3D structure databases

ProteinModelPortaliQ20719.
SMRiQ20719. Positions 44-202.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 24 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1905.
GeneTreeiENSGT00390000017580.
HOGENOMiHOG000257748.
InParanoidiQ20719.
KOiK03943.
OMAiLGEMECM.
PhylomeDBiQ20719.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR002023. NuoE-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PIRSFiPIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
PROSITEiPS01099. COMPLEX1_24K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q20719-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLASNVLLQ ASRLGEMVIK RGGATGLMVH RDTKENNLNV KFKFTSENQE
60 70 80 90 100
RIKAIMDIYP EGHKAGALIP LLDLAQRQHG WLPISAMHEV AKILEVPRMR
110 120 130 140 150
AYEVATFYTM FNRQPVGKYF LQVCATTPCM LRGAETITET IEKKLGIHAG
160 170 180 190 200
ETTKDGLFTL AEVECLGACV NAPMIQINDD YFEDLTPKDV NEILDDLKAG
210 220 230
RKPAAGPRSG RLAAEPFGEL TSLKETPPGP GFGLQAALK
Length:239
Mass (Da):26,241
Last modified:November 1, 1996 - v1
Checksum:i2A63223AFE663E91
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z77663 Genomic DNA. Translation: CAB01203.1.
PIRiT22573.
RefSeqiNP_506376.1. NM_073975.6.
UniGeneiCel.23494.

Genome annotation databases

EnsemblMetazoaiF53F4.10; F53F4.10; WBGene00009992.
GeneIDi179850.
KEGGicel:CELE_F53F4.10.
UCSCiF53F4.10.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z77663 Genomic DNA. Translation: CAB01203.1.
PIRiT22573.
RefSeqiNP_506376.1. NM_073975.6.
UniGeneiCel.23494.

3D structure databases

ProteinModelPortaliQ20719.
SMRiQ20719. Positions 44-202.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi44866. 1 interaction.
DIPiDIP-26781N.
IntActiQ20719. 1 interaction.
MINTiMINT-1083480.
STRINGi6239.F53F4.10.2.

2D gel databases

World-2DPAGE0020:Q20719.

Proteomic databases

PaxDbiQ20719.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF53F4.10; F53F4.10; WBGene00009992.
GeneIDi179850.
KEGGicel:CELE_F53F4.10.
UCSCiF53F4.10.1. c. elegans.

Organism-specific databases

CTDi179850.
WormBaseiF53F4.10; CE10972; WBGene00009992.

Phylogenomic databases

eggNOGiCOG1905.
GeneTreeiENSGT00390000017580.
HOGENOMiHOG000257748.
InParanoidiQ20719.
KOiK03943.
OMAiLGEMECM.
PhylomeDBiQ20719.

Miscellaneous databases

NextBioi907116.
PROiQ20719.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR002023. NuoE-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PIRSFiPIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
PROSITEiPS01099. COMPLEX1_24K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiNDUV2_CAEEL
AccessioniPrimary (citable) accession number: Q20719
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.