Q20719 (NDUV2_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
EC=1.6.5.3
EC=1.6.99.3

Gene names

ORF Names:

F53F4.10

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster Potential.
Subunit structure	Complex I is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme By similarity.
Subcellular location	Mitochondrion inner membrane By similarity.
Sequence similarities	Belongs to the complex I 24 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial electron transport, NADH to ubiquinone Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	mitochondrial respiratory chain complex I Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from Biological aspect of Ancestor. Source: RefGenome metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 239		Probable NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial		PRO_0000020006
Sites
	Metal binding	124	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	129	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	165	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	169	1	Iron-sulfur (2Fe-2S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q20719 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2A63223AFE663E91	FASTA	239	26,241
	10 20 30 40 50 60 MSLASNVLLQ ASRLGEMVIK RGGATGLMVH RDTKENNLNV KFKFTSENQE RIKAIMDIYP 70 80 90 100 110 120 EGHKAGALIP LLDLAQRQHG WLPISAMHEV AKILEVPRMR AYEVATFYTM FNRQPVGKYF 130 140 150 160 170 180 LQVCATTPCM LRGAETITET IEKKLGIHAG ETTKDGLFTL AEVECLGACV NAPMIQINDD 190 200 210 220 230 YFEDLTPKDV NEILDDLKAG RKPAAGPRSG RLAAEPFGEL TSLKETPPGP GFGLQAALK

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z77663 Genomic DNA. Translation: CAB01203.1.
PIR	T22573.
RefSeq	NP_506376.1. NM_073975.6.
3D structure databases
ProteinModelPortal	Q20719.
SMR	Q20719. Positions 44-202.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-26781N.
IntAct	Q20719. 1 interaction.
MINT	MINT-1083480.
STRING	6239.F53F4.10.2.
2D gel databases
World-2DPAGE	0020:Q20719.
Proteomic databases
PaxDb	Q20719.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F53F4.10.1; F53F4.10.1; F53F4.10. F53F4.10.2; F53F4.10.2; F53F4.10.
GeneID	179850.
KEGG	cel:CELE_F53F4.10.
UCSC	F53F4.10.1. c. elegans.
Organism-specific databases
CTD	179850.
WormBase	F53F4.10; CE10972; WBGene00009992.
Phylogenomic databases
eggNOG	COG1905.
GeneTree	ENSGT00390000017580.
HOGENOM	HOG000257748.
InParanoid	Q20719.
KO	K03943.
OMA	KPGPRNG.
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR002023. 2Fe-2S_Ferredox. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR10371. PTHR10371. 1 hit.
Pfam	PF01257. Complex1_24kDa. 1 hit. [Graphical view]
PIRSF	PIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR01958. nuoE_fam. 1 hit.
PROSITE	PS01099. COMPLEX1_24K. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	907116.

Entry information

Entry name

NDUV2_CAEEL

Accession

Primary (citable) accession number: Q20719

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project