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Protein

Peroxidase skpo-1

Gene

skpo-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in hypodermal immune response against some types of bacterial infection. Probably utilizes H2O2 produced by the NADPH oxidase bli-3. May play a role in cuticule biosynthesis.1 Publication1 Publication

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei222Proton acceptorPROSITE-ProRule annotation1
Sitei332Transition state stabilizerPROSITE-ProRule annotation1
Metal bindingi428Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

  • heme binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • peroxidase activity Source: UniProtKB

GO - Biological processi

  • defense response to Gram-positive bacterium Source: UniProtKB
  • determination of adult lifespan Source: UniProtKB
  • innate immune response Source: UniProtKB-KW
  • negative regulation of gene expression Source: UniProtKB
  • response to oxidative stress Source: InterPro

Keywordsi

Molecular functionOxidoreductase, Peroxidase
Biological processImmunity, Innate immunity
LigandHeme, Iron, Metal-binding

Protein family/group databases

PeroxiBasei4139 CelPxd03

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxidase skpo-11 Publication (EC:1.11.1.7By similarity)
Alternative name(s):
ShKT and peroxidase domain-containing protein 1Imported
Gene namesi
Name:skpo-1Imported
ORF Names:F49E12.1Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiF49E12.1 ; CE03378 ; WBGene00009897 ; skpo-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Knockouts show increased susceptibility to infection with E.faecalis but not with P.aeruginosa, increased levels of H2O2 upon infection and a slightly shorter life span. Fertile animals show a bagging phenotype due to embryos being retained in the body and an increase of clec-60 mRNA levels upon infection. 50% of knockouts also have a dumpy phenotype. RNAi-mediated knockdown of the protein also results in higher susceptibility to infection by E.faecalis but not in a decreased life span or morphological changes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000043123920 – 655Peroxidase skpo-1Sequence analysisAdd BLAST636

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi22 ↔ 56PROSITE-ProRule annotation
Disulfide bondi29 ↔ 49PROSITE-ProRule annotation
Disulfide bondi38 ↔ 53PROSITE-ProRule annotation
Disulfide bondi133 ↔ 150PROSITE-ProRule annotation
Disulfide bondi520 ↔ 576PROSITE-ProRule annotation
Disulfide bondi617 ↔ 642PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiQ20616
PaxDbiQ20616
PeptideAtlasiQ20616

Expressioni

Tissue specificityi

Exclusively expressed in hypodermis.1 Publication

Gene expression databases

BgeeiWBGene00009897

Interactioni

Protein-protein interaction databases

STRINGi6239.F49E12.1

Structurei

3D structure databases

ProteinModelPortaliQ20616
SMRiQ20616
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 56ShKTPROSITE-ProRule annotationAdd BLAST35

Sequence similaritiesi

Belongs to the peroxidase family. XPO subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2408 Eukaryota
ENOG410XPZ3 LUCA
GeneTreeiENSGT00550000074325
HOGENOMiHOG000016084
InParanoidiQ20616
OMAiCQTGDNM
OrthoDBiEOG091G03DI
PhylomeDBiQ20616

Family and domain databases

Gene3Di1.10.640.10, 1 hit
InterProiView protein in InterPro
IPR010255 Haem_peroxidase
IPR019791 Haem_peroxidase_animal
IPR037120 Haem_peroxidase_sf
IPR003582 ShKT_dom
PfamiView protein in Pfam
PF03098 An_peroxidase, 1 hit
PF01549 ShK, 1 hit
PRINTSiPR00457 ANPEROXIDASE
SMARTiView protein in SMART
SM00254 ShKT, 1 hit
SUPFAMiSSF48113 SSF48113, 1 hit
PROSITEiView protein in PROSITE
PS50292 PEROXIDASE_3, 1 hit
PS51670 SHKT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q20616-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSLLFSILL IYLIQLVRSE ECTDKHIHCF FWSQEGECEV NPRWMKKHCQ
60 70 80 90 100
KACGTCSLTS PTPLTRQQDV PTARTFDDQQ DRQFLPSRPS SIPEGCNSVM
110 120 130 140 150
TVEAETRRIF SSGQLTARFR QQMCAEEQVA PDCSINQCFH KKYRSMDGTC
160 170 180 190 200
NNLQNPVKGA AFTAFTRLMP AAYDDGFNTL VSASRRNRPN PREVSVFLLS
210 220 230 240 250
SERSLPGHVN SLLMLFGQFV SHDITSNAAQ NFCGCQNSGP MCASIFAPPS
260 270 280 290 300
DRSRRCIPFT RSFPICGTGQ FGRVREQLNM NTAAIDASLI YGSEAITARS
310 320 330 340 350
LRFAAMLRTS MIGGRMFPPN TNPGSLTAGD GRAILFVGLA ALHTSFLRLH
360 370 380 390 400
NNVAARLQNM NRHWNADRIF QESRKIVGGI VQVITYQEFV PELIGDASKT
410 420 430 440 450
ILGAYNGYNP NVEIGVLNEF AAGAYRLHGM IQETYPLVNS QFQEVNRYRF
460 470 480 490 500
IDGVNNINHV LNNIDAIYRG MMTVPVRSPQ RLTTSVTERL FGGSVDMAAV
510 520 530 540 550
NIQRGRDHGL RSYNDYRRFC NLRPITSFND WPEVPDENVR QRIGQLYRTP
560 570 580 590 600
DDLDFYVGGI LEQPAAGSLL GATFACVIGK QFERLRDGDR FYYENPGVFT
610 620 630 640 650
SPQLAELKRT TLSWVLCQTG DNMVRVGRRA FDIENGSRAV PCSSITGLNL

EAWRE
Length:655
Mass (Da):73,496
Last modified:November 1, 1996 - v1
Checksum:iC8225BAFF8E2164E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z66520 Genomic DNA Translation: CAA91388.1
PIRiT22448
RefSeqiNP_495768.1, NM_063367.5
UniGeneiCel.5463

Genome annotation databases

EnsemblMetazoaiF49E12.1; F49E12.1; WBGene00009897
GeneIDi174340
KEGGicel:CELE_F49E12.1
UCSCiF49E12.1 c. elegans

Similar proteinsi

Entry informationi

Entry nameiSKPO1_CAEEL
AccessioniPrimary (citable) accession number: Q20616
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 26, 2014
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

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