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Protein

C-terminal-binding protein 1

Gene

ctbp-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds DNA and represses gene expression. Plays a role in regulation of life span, possibly by regulating transcription of genes important for lipid metabolism.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei251 – 2511NADBy similarity
Binding sitei355 – 3551NADBy similarity
Binding sitei441 – 4411NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri5 – 6056THAP-typePROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi331 – 3366NADBy similarity
Nucleotide bindingi388 – 3947NADBy similarity
Nucleotide bindingi415 – 4173NADBy similarity
Nucleotide bindingi467 – 4704NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, NAD, Zinc

Enzyme and pathway databases

ReactomeiR-CEL-3769402. Deactivation of the beta-catenin transactivating complex.
R-CEL-4641265. Repression of WNT target genes.

Names & Taxonomyi

Protein namesi
Recommended name:
C-terminal-binding protein 11 Publication
Gene namesi
Name:ctbp-1Imported
ORF Names:F49E10.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiF49E10.5a; CE29966; WBGene00006424; ctbp-1.
F49E10.5b; CE47412; WBGene00006424; ctbp-1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: WormBase
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Adult life span extension and increased resistance to oxidative and heat stress but not to DNA damage, starvation or pathogen stress.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi332 – 3321G → V: Does not rescue the extended lifespan phenotype observed in ctbp-1 deletion mutants. 1 Publication
Mutagenesisi334 – 3341G → V: Does not rescue the extended lifespan phenotype observed in ctbp-1 deletion mutants. 1 Publication
Mutagenesisi467 – 4671H → A: Rescues the extended lifespan phenotype observed in ctbp-1 deletion mutants. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 727727C-terminal-binding protein 1PRO_0000424056Add
BLAST

Proteomic databases

PaxDbiQ20595.
PRIDEiQ20595.

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

STRINGi6239.F49E10.5.

Structurei

Secondary structure

1
727
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni13 – 153Combined sources
Beta strandi16 – 183Combined sources
Helixi33 – 4311Combined sources
Helixi47 – 493Combined sources
Beta strandi50 – 534Combined sources
Helixi58 – 603Combined sources
Beta strandi67 – 704Combined sources
Turni76 – 783Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JM3NMR-A1-89[»]
ProteinModelPortaliQ20595.
SMRiQ20595. Positions 1-89, 179-505.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ20595.

Family & Domainsi

Domaini

The THAP-type zinc finger mediates DNA-binding but is not required for repression of gene expression.2 Publications

Sequence similaritiesi

Contains 1 THAP-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri5 – 6056THAP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0067. Eukaryota.
COG0111. LUCA.
GeneTreeiENSGT00530000063021.
HOGENOMiHOG000020404.
InParanoidiQ20595.
KOiK04496.
OMAiIAVCHAP.
OrthoDBiEOG761BT9.
PhylomeDBiQ20595.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029752. D-isomer_DH_CS1.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR006612. Znf_C2CH.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
PF05485. THAP. 1 hit.
[Graphical view]
SMARTiSM00980. THAP. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS50950. ZF_THAP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a1 Publication (identifier: Q20595-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPTTCGFPNC KFRSRYRGLE DNRHFYRIPK RPLILRQRWL TAIGRTEETV
60 70 80 90 100
VSQLRICSAH FEGGEKKEGD IPVPDPTVDK QIKIELPPKE SKNSDRRRKQ
110 120 130 140 150
NIPARFPRPE SPSGDSPSYS KKSRSFRDFY PSLSSTPSFD PAQSPHTPHP
160 170 180 190 200
PVLPDPQQAL NDILSMTSTR MNGPSSSRPL VALLDGRDCS VEMPILKDVA
210 220 230 240 250
TVAFCDAQST QEIHEKVLNE AVAALMYHSI KLEKEDLEKF KVLKVVFRIG
260 270 280 290 300
YGIDNIDVKA ATELGIAVCH APGDYVEDVA DSTLSLILDL FRRTYWHAKS
310 320 330 340 350
YSETRKTIGA DQVRENAVGS KKVRGSVLGI LGCGRVGTAV GLRARAFGLH
360 370 380 390 400
IIFYDPFVRE GHDKALGFER VYTMDEFMSR SDCISLHCNL GDETRGIINA
410 420 430 440 450
DSLRQCKSGV YIVNTSHAGL INENDLAAAL KNGHVKGAAL DVHDSVRFDP
460 470 480 490 500
NCLNPLVGCP NIINTPHSAW MTEASCKDLR INAAKEIRKA INGRCPQDLT
510 520 530 540 550
HCINKEAVMR NSNPINRRTS SAHPLLNMGF PTLPNFPPMS MSPHFPYPNP
560 570 580 590 600
LLAMGAQMGA LNPFMGNGAL PFNPAAALSS LAAAQAANAQ RGSPANRSSR
610 620 630 640 650
SSPSPHTNKS SVSPGNNGHV KTEPSSPAAK IEVDIAENDK HSMMTFLQRL
660 670 680 690 700
IAPNGDSGAS TADSGIEGGD KEKVQSDGDE NMEDMEVIDA EKLKEELNIG
710 720
QLEEPEEISV GLNNGNRINI DEQPLAT
Length:727
Mass (Da):79,598
Last modified:March 1, 2002 - v3
Checksum:i33D6FC34EE01A371
GO
Isoform b1 Publication (identifier: Q20595-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-121: Missing.
     122-140: KSRSFRDFYPSLSSTPSFD → MGGEANGTKPTGSQKRKRN

Show »
Length:606
Mass (Da):65,388
Checksum:iA0CAC98009E3EBEE
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 121121Missing in isoform b. 1 PublicationVSP_053308Add
BLAST
Alternative sequencei122 – 14019KSRSF…TPSFD → MGGEANGTKPTGSQKRKRN in isoform b. 1 PublicationVSP_053309Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081427 Genomic DNA. Translation: CCD71569.1.
FO081427 Genomic DNA. Translation: CCG28183.1.
PIRiT34289.
RefSeqiNP_001257030.1. NM_001270101.1. [Q20595-1]
NP_001257031.1. NM_001270102.1. [Q20595-2]
UniGeneiCel.17039.

Genome annotation databases

EnsemblMetazoaiF49E10.5a; F49E10.5a; WBGene00006424. [Q20595-1]
GeneIDi180853.
KEGGicel:CELE_F49E10.5.
UCSCiF49E10.5. c. elegans. [Q20595-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081427 Genomic DNA. Translation: CCD71569.1.
FO081427 Genomic DNA. Translation: CCG28183.1.
PIRiT34289.
RefSeqiNP_001257030.1. NM_001270101.1. [Q20595-1]
NP_001257031.1. NM_001270102.1. [Q20595-2]
UniGeneiCel.17039.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JM3NMR-A1-89[»]
ProteinModelPortaliQ20595.
SMRiQ20595. Positions 1-89, 179-505.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.F49E10.5.

Proteomic databases

PaxDbiQ20595.
PRIDEiQ20595.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF49E10.5a; F49E10.5a; WBGene00006424. [Q20595-1]
GeneIDi180853.
KEGGicel:CELE_F49E10.5.
UCSCiF49E10.5. c. elegans. [Q20595-1]

Organism-specific databases

CTDi180853.
WormBaseiF49E10.5a; CE29966; WBGene00006424; ctbp-1.
F49E10.5b; CE47412; WBGene00006424; ctbp-1.

Phylogenomic databases

eggNOGiKOG0067. Eukaryota.
COG0111. LUCA.
GeneTreeiENSGT00530000063021.
HOGENOMiHOG000020404.
InParanoidiQ20595.
KOiK04496.
OMAiIAVCHAP.
OrthoDBiEOG761BT9.
PhylomeDBiQ20595.

Enzyme and pathway databases

ReactomeiR-CEL-3769402. Deactivation of the beta-catenin transactivating complex.
R-CEL-4641265. Repression of WNT target genes.

Miscellaneous databases

EvolutionaryTraceiQ20595.
PROiQ20595.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029752. D-isomer_DH_CS1.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR006612. Znf_C2CH.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
PF05485. THAP. 1 hit.
[Graphical view]
SMARTiSM00980. THAP. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS50950. ZF_THAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2Imported.
  2. "The Caenorhabditis elegans protein CTBP-1 defines a new group of THAP domain-containing CtBP corepressors."
    Nicholas H.R., Lowry J.A., Wu T., Crossley M.
    J. Mol. Biol. 375:1-11(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, DOMAIN.
  3. "The conserved NAD(H)-dependent corepressor CTBP-1 regulates Caenorhabditis elegans life span."
    Chen S., Whetstine J.R., Ghosh S., Hanover J.A., Gali R.R., Grosu P., Shi Y.
    Proc. Natl. Acad. Sci. U.S.A. 106:1496-1501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-332; GLY-334 AND HIS-467.
  4. "Solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP)."
    Liew C.K., Crossley M., Mackay J.P., Nicholas H.R.
    J. Mol. Biol. 366:382-390(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 1-89 IN COMPLEX WITH ZINC, DOMAIN, DNA-BINDING.

Entry informationi

Entry nameiCTBP1_CAEEL
AccessioniPrimary (citable) accession number: Q20595
Secondary accession number(s): H8W3Z5, Q20596
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: March 1, 2002
Last modified: June 8, 2016
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Caution

In contrast to other members of the family, lacks the conserved Arg and Glu active sites at positions 417 and 446 respectively, suggesting that it lacks dehydrogenase activity.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.