ID   HUTH_CAEEL              Reviewed;         677 AA.
AC   Q20502;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 58.
DE   RecName: Full=Probable histidine ammonia-lyase;
DE            Short=Histidase;
DE            EC=4.3.1.3;
GN   ORFNames=F47B10.2;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Nematoda; Chromadorea; Rhabditida; Rhabditoidea;
OC   Rhabditidae; Peloderinae; Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   MEDLINE=99069613; PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for
RT   investigating biology.";
RL   Science 282:2012-2018(1998).
CC   -!- CATALYTIC ACTIVITY: L-histidine = urocanate + NH(3).
CC   -!- PATHWAY: Amino-acid degradation; L-histidine degradation into L-
CC       glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3.
CC   -!- INTERACTION:
CC       Q9TZ38:gei-4; NbExp=1; IntAct=EBI-322047, EBI-329192;
CC   -!- PTM: Contains an active site 4-methylidene-imidazol-5-one (MIO),
CC       which is formed autocatalytically by cyclization and dehydration
CC       of residues Cys-Ser-Gly (By similarity).
CC   -!- SIMILARITY: Belongs to the PAL/histidase family.
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DR   EMBL; Z68004; CAA91982.1; -; Genomic_DNA.
DR   PIR; T22333; T22333.
DR   RefSeq; NP_509820.1; -.
DR   UniGene; Cel.9060; -.
DR   HSSP; P21310; 1GKM.
DR   DIP; DIP:27218N; -.
DR   IntAct; Q20502; 2.
DR   Ensembl; F47B10.2; Caenorhabditis elegans.
DR   GeneID; 181279; -.
DR   KEGG; cel:F47B10.2; -.
DR   NMPDR; fig|6239.3.peg.24527; -.
DR   WormBase; WBGene00009813; F47B10.2.
DR   WormPep; F47B10.2; CE03352.
DR   OMA; Q20502; HCTAASL.
DR   BRENDA; 4.3.1.3; 672.
DR   NextBio; 913256; -.
DR   ArrayExpress; Q20502; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0016211; F:ammonia ligase activity; IEA:InterPro.
DR   GO; GO:0004397; F:histidine ammonia-lyase activity; IEA:EC.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   GO; GO:0006548; P:histidine catabolic process; IEA:InterPro.
DR   InterPro; IPR005921; HutH.
DR   InterPro; IPR001106; Phe/His_NH3-lyase.
DR   Pfam; PF00221; PAL; 1.
DR   TIGRFAMs; TIGR01225; hutH; 1.
DR   PROSITE; PS00488; PAL_HISTIDASE; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Histidine metabolism; Lyase.
FT   CHAIN         1    677       Probable histidine ammonia-lyase.
FT                                /FTId=PRO_0000161061.
FT   MOD_RES     270    270       2,3-didehydroalanine (Ser) (By
FT                                similarity).
FT   CROSSLNK    269    271       5-imidazolinone (Cys-Gly) (By
FT                                similarity).
SQ   SEQUENCE   677 AA;  74635 MW;  E64CCD5B097AAC4F CRC64;
     MRLQVQIGTE CVVVPCKPDD TIHAVAKKSV EKLRRLRPKL PLADDYFEVR RTVGNSLLDP
     EDLVSDVLKD SDFIIVAASV EETEDAKEAK KQEEIDNARA EIEKIDNRRR KVSFADSLAP
     MVLAPPTKLL ILDGNSLLPE DLVRCEKGEC AIQLSMESED RIRKARTFLE KIASEHRAVY
     GVTTGFGTFS NVTIPPEKLK KLQLNLIRSH ATGYGEPLAP NRARMLLALR INILAKGHSG
     ISVENIKKMI AAFNAFCVSY VPQQGTVGCS GDLCPLAHLA LGLLGEGKMW SPTTGWQPAD
     VVLKKNNLEP LELGPKEGLA LINGTQMVTA LGAYTLERAH NIARQADVIA ALSLDVLKGT
     TRAYDPDIHR IRPHRGQNLS ALRLRALLHS EANPSQIAES HRNCTKVQDA YTLRCVPQVH
     GVVHDTIEFV REIITTEMNS ATDNPLVFAD REEIISGGNF HGEYPAKALD FLAIAVAELA
     QMSERRLERL VNKELSGLPT FLTPDGGLNS GFMTVQLCAA SLVSENKVLC HPSSVDSIPT
     SCNQEDHVSM GGFAARKALT VVEHVEAVLA MELLAACQGI EFLKPLISTA PLHKIYQLVR
     SVAPPLNEDR YMKPEIDAVL EMIRENRIWE AVLPHLETLE AMEELDPDAL RQFTKTPTGI
     VQDRSMIPIS DDEESIE
//