Q20502 (HUTH_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable histidine ammonia-lyase
Short name=Histidase
EC=4.3.1.3

Gene names

ORF Names:

F47B10.2

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	677 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-histidine = urocanate + NH3.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3.
Post-translational modification	Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Cys-Ser-Gly By similarity.
Sequence similarities	Belongs to the PAL/histidase family.

Ontologies

Keywords
Biological process	Histidine metabolism
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro histidine catabolic process Inferred from mutant phenotype. Source: WormBase zinc ion homeostasis Inferred from mutant phenotype. Source: WormBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	histidine ammonia-lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
gei-4	Q9TZ38	1	EBI-322047,EBI-329192

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 677	677	Probable histidine ammonia-lyase		PRO_0000161061
Amino acid modifications
	Modified residue	270	1	2,3-didehydroalanine (Ser) By similarity
	Cross-link	269 ↔ 271		5-imidazolinone (Cys-Gly) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q20502 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E64CCD5B097AAC4F	FASTA	677	74,635
	10 20 30 40 50 60 MRLQVQIGTE CVVVPCKPDD TIHAVAKKSV EKLRRLRPKL PLADDYFEVR RTVGNSLLDP 70 80 90 100 110 120 EDLVSDVLKD SDFIIVAASV EETEDAKEAK KQEEIDNARA EIEKIDNRRR KVSFADSLAP 130 140 150 160 170 180 MVLAPPTKLL ILDGNSLLPE DLVRCEKGEC AIQLSMESED RIRKARTFLE KIASEHRAVY 190 200 210 220 230 240 GVTTGFGTFS NVTIPPEKLK KLQLNLIRSH ATGYGEPLAP NRARMLLALR INILAKGHSG 250 260 270 280 290 300 ISVENIKKMI AAFNAFCVSY VPQQGTVGCS GDLCPLAHLA LGLLGEGKMW SPTTGWQPAD 310 320 330 340 350 360 VVLKKNNLEP LELGPKEGLA LINGTQMVTA LGAYTLERAH NIARQADVIA ALSLDVLKGT 370 380 390 400 410 420 TRAYDPDIHR IRPHRGQNLS ALRLRALLHS EANPSQIAES HRNCTKVQDA YTLRCVPQVH 430 440 450 460 470 480 GVVHDTIEFV REIITTEMNS ATDNPLVFAD REEIISGGNF HGEYPAKALD FLAIAVAELA 490 500 510 520 530 540 QMSERRLERL VNKELSGLPT FLTPDGGLNS GFMTVQLCAA SLVSENKVLC HPSSVDSIPT 550 560 570 580 590 600 SCNQEDHVSM GGFAARKALT VVEHVEAVLA MELLAACQGI EFLKPLISTA PLHKIYQLVR 610 620 630 640 650 660 SVAPPLNEDR YMKPEIDAVL EMIRENRIWE AVLPHLETLE AMEELDPDAL RQFTKTPTGI 670 VQDRSMIPIS DDEESIE

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z68004 Genomic DNA. Translation: CAA91982.1.
PIR	T22333.
RefSeq	NP_509820.1. NM_077419.5.
UniGene	Cel.9060.
3D structure databases
ProteinModelPortal	Q20502.
SMR	Q20502. Positions 1-78, 127-673.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-27218N.
IntAct	Q20502. 2 interactions.
MINT	MINT-117897.
STRING	Q20502.
Proteomic databases
PRIDE	Q20502.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F47B10.2; F47B10.2; F47B10.2.
GeneID	181279.
KEGG	cel:F47B10.2.
NMPDR	fig|6239.3.peg.24527.
UCSC	F47B10.2. c. elegans.
Organism-specific databases
CTD	181279.
WormBase	F47B10.2; CE03352; WBGene00009813.
Phylogenomic databases
eggNOG	meNOG04759.
GeneTree	EMGT00050000019109.
HOGENOM	HBG510887.
InParanoid	Q20502.
OMA	CKPDDTI.
PhylomeDB	Q20502.
Gene expression databases
ArrayExpress	Q20502.
Family and domain databases
InterPro	IPR005921. HutH. IPR008948. L-Aspartase-like. IPR024083. L-Aspartase-like_N. IPR001106. Phe/His_NH3-lyase. IPR022313. Phe/His_NH3-lyase_AS. [Graphical view]
Gene3D	G3DSA:1.10.275.10. G3DSA:1.10.275.10. 1 hit.
KO	K01745.
Pfam	PF00221. PAL. 1 hit. [Graphical view]
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR01225. HutH. 1 hit.
PROSITE	PS00488. PAL_HISTIDASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	913256.

Entry information

Entry name

HUTH_CAEEL

Accession

Primary (citable) accession number: Q20502

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project