Reviewed, UniProtKB/Swiss-Prot Q20502 (HUTH_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable histidine ammonia-lyase
      Short name=Histidase
    EC=4.3.1.3

Gene names

ORF Names:

F47B10.2

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	677 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-histidine = urocanate + NH3.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3.
Post-translational modification	Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Cys-Ser-Gly By similarity.
Sequence similarities	Belongs to the PAL/histidase family.

Ontologies

Keywords
Biological process	Histidine metabolism
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro histidine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ammonia ligase activity Inferred from electronic annotation. Source: InterPro histidine ammonia-lyase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
gei-4	Q9TZ38	1	EBI-322047,EBI-329192

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 677	677	Probable histidine ammonia-lyase		PRO_0000161061
Amino acid modifications
	Modified residue	270	1	2,3-didehydroalanine (Ser) By similarity
	Cross-link	269 ↔ 271		5-imidazolinone (Cys-Gly) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q20502-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E64CCD5B097AAC4F	FASTA	677	74,635
	10 20 30 40 50 60 MRLQVQIGTE CVVVPCKPDD TIHAVAKKSV EKLRRLRPKL PLADDYFEVR RTVGNSLLDP 70 80 90 100 110 120 EDLVSDVLKD SDFIIVAASV EETEDAKEAK KQEEIDNARA EIEKIDNRRR KVSFADSLAP 130 140 150 160 170 180 MVLAPPTKLL ILDGNSLLPE DLVRCEKGEC AIQLSMESED RIRKARTFLE KIASEHRAVY 190 200 210 220 230 240 GVTTGFGTFS NVTIPPEKLK KLQLNLIRSH ATGYGEPLAP NRARMLLALR INILAKGHSG 250 260 270 280 290 300 ISVENIKKMI AAFNAFCVSY VPQQGTVGCS GDLCPLAHLA LGLLGEGKMW SPTTGWQPAD 310 320 330 340 350 360 VVLKKNNLEP LELGPKEGLA LINGTQMVTA LGAYTLERAH NIARQADVIA ALSLDVLKGT 370 380 390 400 410 420 TRAYDPDIHR IRPHRGQNLS ALRLRALLHS EANPSQIAES HRNCTKVQDA YTLRCVPQVH 430 440 450 460 470 480 GVVHDTIEFV REIITTEMNS ATDNPLVFAD REEIISGGNF HGEYPAKALD FLAIAVAELA 490 500 510 520 530 540 QMSERRLERL VNKELSGLPT FLTPDGGLNS GFMTVQLCAA SLVSENKVLC HPSSVDSIPT 550 560 570 580 590 600 SCNQEDHVSM GGFAARKALT VVEHVEAVLA MELLAACQGI EFLKPLISTA PLHKIYQLVR 610 620 630 640 650 660 SVAPPLNEDR YMKPEIDAVL EMIRENRIWE AVLPHLETLE AMEELDPDAL RQFTKTPTGI 670 VQDRSMIPIS DDEESIE

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z68004 Genomic DNA. Translation: CAA91982.1.
PIR	T22333.
RefSeq	NP_509820.1.
UniGene	Cel.9060
3D structure databases
HSSP	HSSP built from PDB template 1GKM based on UniProtKB P21310.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:27218N.
IntAct	Q20502. 2 interactions.
Genome annotation databases
Ensembl	F47B10.2. Caenorhabditis elegans. [Contig view]
GeneID	181279.
KEGG	cel:F47B10.2.
NMPDR	fig|6239.3.peg.24527.
Organism-specific databases
WormBase	WBGene00009813. F47B10.2.
WormPep	F47B10.2. CE03352. [WorfDB]
Phylogenomic databases
OMA	Q20502. HCTAASL.
Enzyme and pathway databases
BRENDA	4.3.1.3. 672.
Gene expression databases
ArrayExpress	Q20502.
Family and domain databases
InterPro	IPR005921. HutH. IPR001106. Phe/His_NH3-lyase. [Graphical view]
Pfam	PF00221. PAL. 1 hit. [Graphical view]
TIGRFAMs	TIGR01225. hutH. 1 hit.
PROSITE	PS00488. PAL_HISTIDASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	913256.

Entry information

Entry name

HUTH_CAEEL

Accession

Primary (citable) accession number: Q20502

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project