Reviewed, UniProtKB/Swiss-Prot Q20459 (NAS25_CAEEL)

Names and origin

Protein names

Recommended name:
    Zinc metalloproteinase nas-25
    EC=3.4.24.21
Alternative name(s):
    Nematode astacin 25

Gene names

Name:	nas-25
ORF Names:	F46C5.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable metalloprotease By similarity.
Catalytic activity	Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the peptidase M12A family. Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	EGF-like domain Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 399	379	Zinc metalloproteinase nas-25		PRO_0000028929
Regions
	Domain	232 – 275	44	EGF-like
Sites
	Active site	135	1	By similarity
	Metal binding	134	1	Zinc; catalytic By similarity
	Metal binding	138	1	Zinc; catalytic By similarity
	Metal binding	144	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	52	1	N-linked (GlcNAc...) Potential
	Glycosylation	61	1	N-linked (GlcNAc...) Potential
	Glycosylation	371	1	N-linked (GlcNAc...) Potential
	Disulfide bond	236 ↔ 249		By similarity
	Disulfide bond	240 ↔ 260		By similarity
	Disulfide bond	265 ↔ 274		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q20459-1 [UniParc]. Last modified December 1, 2001. Version 3. Checksum: 38EA62319A2CF172	FASTA	399	45,456
	10 20 30 40 50 60 MQIYLGITIC LVAFLTVIDC AIPYYRTHSN FGSLGRRKVR QVQRDLTYRW PNNTVPYYVG 70 80 90 100 110 120 NVTSTIKKSV RLAIEELQAW TCIRFQNVNE KYSDGDSVRI VDLGSCSSPI GRQQIGTQDV 130 140 150 160 170 180 SLTKNCWGMG TAIHELMHAI GIEHTQSRSD RNRYLDILAQ NIDNRDLPNF ELLSPRLWAN 190 200 210 220 230 240 LVPYDYGSVM HYSADSFSNK DDEQTMLPKD RSFIETMGSM IPNFYDFDQI NQYYQCYDSC 250 260 270 280 290 300 RNAGQLANCA NGGIPNPNNC QVCNCPMGYG GDLCDQRPEG CGSTLVATDR WQKQKLSVRF 310 320 330 340 350 360 SRNDDQYFTF CNSWIVGPSD RTLQVIYEIT SDSIRRQICS FGCYEGGIEV KHLQDPRITN 370 380 390 DRDCCLNTPL NLTTTVNPLP VILYTSGATV TYDFSYRYV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"The astacin protein family in Caenorhabditis elegans." Moehrlen F., Hutter H., Zwilling R. Eur. J. Biochem. 270:4909-4920(2003) [PubMed: 14653817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 208-260, NOMENCLATURE. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z54281 Genomic DNA. Translation: CAA91045.3. AJ561215 mRNA. Translation: CAD99216.1.
PIR	T22300.
RefSeq	NP_495880.1.
UniGene	Cel.15073
3D structure databases
HSSP	HSSP built from PDB template 1IAE based on UniProtKB P07584.
ModBase	Search...
Protein family/group databases
MEROPS	M12.310.
Genome annotation databases
Ensembl	F46C5.3. Caenorhabditis elegans. [Contig view]
GeneID	174412.
KEGG	cel:F46C5.3.
NMPDR	fig|6239.3.peg.6739.
Organism-specific databases
WormBase	WBGene00003544. nas-25.
WormPep	F46C5.3. CE28935. [WorfDB]
Phylogenomic databases
OMA	Q20459. ANCANGG.
Enzyme and pathway databases
BRENDA	3.4.24.21. 672.
Gene expression databases
ArrayExpress	Q20459.
Family and domain databases
InterPro	IPR013032. EGF-like_reg_CS. IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR001506. Peptidase_M12A. IPR017050. Peptidase_M12A_nem. [Graphical view]
Pfam	PF01400. Astacin. 1 hit. [Graphical view]
PIRSF	PIRSF036365. Astacin_nematoda. 1 hit.
PRINTS	PR00480. ASTACIN.
SMART	SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	883914.

Entry information

Entry name

NAS25_CAEEL

Accession

Primary (citable) accession number: Q20459
Secondary accession number(s): Q7Z0M5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 61 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project