Q20432 (CNEP1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CTD nuclear envelope phosphatase 1 homolog
EC=3.1.3.16

Alternative name(s):
Serine/threonine-protein phosphatase dullard homolog
Small C-terminal domain phosphatase-like phosphatase 2

Gene names

Name:	scpl-2
ORF Names:	F45E12.1

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Serine/threonine protein phosphatase that may dephosphorylate and activate lipin-like phosphatases. Lipins are phosphatidate phosphatases that catalyze the conversion of phosphatidic acid to diacylglycerol and control the metabolism of fatty acids at differents levels. May indirectly modulate the lipid composition of nuclear and/or endoplasmic reticulum membranes and be required for proper nuclear membrane morphology and/or dynamics. May also indirectly regulate the production of lipid droplets and triacylglycerol. Ref.3
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the dullard family. Contains 1 FCP1 homology domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nuclear envelope organization Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of triglyceride biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB protein dephosphorylation Inferred from sequence or structural similarity. Source: UniProtKB regulation of nuclear division Inferred from mutant phenotype Ref.3. Source: WormBase
Cellular_component	Nem1-Spo7 phosphatase complex Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	protein serine/threonine phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	CTD nuclear envelope phosphatase 1 homolog		PRO_0000297975
Regions
	Transmembrane	3 – 23	21	Helical; Potential
	Domain	53 – 220	168	FCP1 homology

Sequences

	Sequence		Length	Mass (Da)
	Q20432 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7D60A06A050EA3EA	FASTA	246	28,414
	10 20 30 40 50 60 MTTIAQSVFC FLAGFFNFFL LYFRKTSRAY CKYQVVKYHS NIPMSPLTTH RLLTVKRKIL 70 80 90 100 110 120 VLDLDETLIH SHHDGVLRQT VKPGTPSDFT IRVVIDRHPV KFSVHERPHV DYFLSVVSQW 130 140 150 160 170 180 YELVVFTASM EVYGTSVADR LDRGRGILKR RYFRQHCTME VGGYTKDLSA IHPDLSSICI 190 200 210 220 230 240 LDNSPGAYRK FPHNAIPIPS WFSDPNDTCL LNLLPFLDAL RFTSDVRSVL SRNMQALPET QSVQYY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Molecular cloning and characterization of dullard: a novel gene required for neural development." Satow R., Chan T.C., Asashima M. Biochem. Biophys. Res. Commun. 295:85-91(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[3]	"Nuclear envelope phosphatase-regulatory subunit 1 (formerly TMEM188) is the metazoan SPO7 ortholog and functions in the lipin activation pathway." Han S., Bahmanyar S., Zhang P., Grishin N., Oegema K., Crooke R., Graham M., Reue K., Dixon J.E., Goodman J.M. J. Biol. Chem. 287:3123-3137(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080734 Genomic DNA. Translation: CCD66263.1.
PIR	T16371.
RefSeq	NP_001254124.1. NM_001267195.1.
UniGene	Cel.15079.
3D structure databases
ProteinModelPortal	Q20432.
SMR	Q20432. Positions 58-232.
ModBase	Search...
Protein-protein interaction databases
STRING	6239.F45E12.1.
Proteomic databases
PaxDb	Q20432.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F45E12.1a; F45E12.1a; F45E12.1.
GeneID	185802.
KEGG	cel:CELE_F45E12.1.
UCSC	F45E12.1. c. elegans.
Organism-specific databases
CTD	185802.
WormBase	F45E12.1; CE02737; WBGene00018474; scpl-2.
Phylogenomic databases
eggNOG	COG5190.
GeneTree	ENSGT00550000075053.
InParanoid	Q20432.
Family and domain databases
Gene3D	3.40.50.1000. 1 hit.
InterPro	IPR011948. Dullard_phosphatase. IPR023214. HAD-like_dom. IPR004274. NIF. [Graphical view]
Pfam	PF03031. NIF. 1 hit. [Graphical view]
SMART	SM00577. CPDc. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR02251. HIF-SF_euk. 1 hit.
PROSITE	PS50969. FCP1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	929562.

Entry information

Entry name

CNEP1_CAEEL

Accession

Primary (citable) accession number: Q20432

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project