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Protein

CTD nuclear envelope phosphatase 1 homolog

Gene

cnep-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Serine/threonine protein phosphatase that may dephosphorylate and activate lipin-like phosphatases. Lipins are phosphatidate phosphatases that catalyze the conversion of phosphatidic acid to diacylglycerol and control the metabolism of fatty acids at differents levels. May indirectly modulate the lipid composition of nuclear and/or endoplasmic reticulum membranes and be required for proper nuclear membrane morphology and/or dynamics. May also indirectly regulate the production of lipid droplets and triacylglycerol.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

GO - Molecular functioni

  1. protein serine/threonine phosphatase activity Source: UniProtKB

GO - Biological processi

  1. nuclear envelope organization Source: UniProtKB
  2. positive regulation of triglyceride biosynthetic process Source: UniProtKB
  3. protein dephosphorylation Source: UniProtKB
  4. regulation of nuclear division Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

ReactomeiREACT_208370. Depolymerisation of the Nuclear Lamina.

Names & Taxonomyi

Protein namesi
Recommended name:
CTD nuclear envelope phosphatase 1 homolog (EC:3.1.3.16)
Short name:
CTDNEP1
Alternative name(s):
Serine/threonine-protein phosphatase dullard homolog
Small C-terminal domain phosphatase-like phosphatase 21
Gene namesi
Name:cnep-1
Synonyms:scpl-2
ORF Names:F45E12.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome II

Organism-specific databases

WormBaseiF45E12.1a; CE02737; WBGene00018474; cnep-1.
F45E12.1b; CE46930; WBGene00018474; cnep-1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2321HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. endoplasmic reticulum membrane Source: UniProtKB
  3. integral component of membrane Source: UniProtKB-KW
  4. Nem1-Spo7 phosphatase complex Source: UniProtKB
  5. nuclear envelope Source: UniProtKB
  6. nuclear membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 246246CTD nuclear envelope phosphatase 1 homologPRO_0000297975Add
BLAST

Proteomic databases

PaxDbiQ20432.

Expressioni

Gene expression databases

ExpressionAtlasiQ20432. baseline.

Interactioni

Protein-protein interaction databases

STRINGi6239.F45E12.1.

Structurei

3D structure databases

ProteinModelPortaliQ20432.
SMRiQ20432. Positions 58-232.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 220168FCP1 homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the dullard family.Curated
Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5190.
GeneTreeiENSGT00550000075053.
InParanoidiQ20432.
KOiK17617.
OMAiRYFRQHC.
PhylomeDBiQ20432.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform a (identifier: Q20432-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTIAQSVFC FLAGFFNFFL LYFRKTSRAY CKYQVVKYHS NIPMSPLTTH
60 70 80 90 100
RLLTVKRKIL VLDLDETLIH SHHDGVLRQT VKPGTPSDFT IRVVIDRHPV
110 120 130 140 150
KFSVHERPHV DYFLSVVSQW YELVVFTASM EVYGTSVADR LDRGRGILKR
160 170 180 190 200
RYFRQHCTME VGGYTKDLSA IHPDLSSICI LDNSPGAYRK FPHNAIPIPS
210 220 230 240
WFSDPNDTCL LNLLPFLDAL RFTSDVRSVL SRNMQALPET QSVQYY

Note: No experimental confirmation available.

Length:246
Mass (Da):28,414
Last modified:November 1, 1996 - v1
Checksum:i7D60A06A050EA3EA
GO
Isoform b (identifier: Q20432-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MYVPNRQFGGGNPESHRRRDQKQASLVLRTM

Note: No experimental confirmation available.

Show »
Length:276
Mass (Da):31,895
Checksum:i1C1AE11E1C600915
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MYVPNRQFGGGNPESHRRRD QKQASLVLRTM in isoform b. CuratedVSP_056100

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080734 Genomic DNA. Translation: CCD66263.1.
FO080734 Genomic DNA. Translation: CCF23398.1.
PIRiT16371.
RefSeqiNP_001254123.1. NM_001267194.1. [Q20432-2]
NP_001254124.1. NM_001267195.1. [Q20432-1]
UniGeneiCel.15079.

Genome annotation databases

EnsemblMetazoaiF45E12.1a; F45E12.1a; WBGene00018474. [Q20432-1]
F45E12.1b; F45E12.1b; WBGene00018474. [Q20432-2]
GeneIDi185802.
KEGGicel:CELE_F45E12.1.
UCSCiF45E12.1. c. elegans. [Q20432-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080734 Genomic DNA. Translation: CCD66263.1.
FO080734 Genomic DNA. Translation: CCF23398.1.
PIRiT16371.
RefSeqiNP_001254123.1. NM_001267194.1. [Q20432-2]
NP_001254124.1. NM_001267195.1. [Q20432-1]
UniGeneiCel.15079.

3D structure databases

ProteinModelPortaliQ20432.
SMRiQ20432. Positions 58-232.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.F45E12.1.

Proteomic databases

PaxDbiQ20432.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF45E12.1a; F45E12.1a; WBGene00018474. [Q20432-1]
F45E12.1b; F45E12.1b; WBGene00018474. [Q20432-2]
GeneIDi185802.
KEGGicel:CELE_F45E12.1.
UCSCiF45E12.1. c. elegans. [Q20432-1]

Organism-specific databases

CTDi185802.
WormBaseiF45E12.1a; CE02737; WBGene00018474; cnep-1.
F45E12.1b; CE46930; WBGene00018474; cnep-1.

Phylogenomic databases

eggNOGiCOG5190.
GeneTreeiENSGT00550000075053.
InParanoidiQ20432.
KOiK17617.
OMAiRYFRQHC.
PhylomeDBiQ20432.

Enzyme and pathway databases

ReactomeiREACT_208370. Depolymerisation of the Nuclear Lamina.

Miscellaneous databases

NextBioi929562.
PROiQ20432.

Gene expression databases

ExpressionAtlasiQ20432. baseline.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  2. "Molecular cloning and characterization of dullard: a novel gene required for neural development."
    Satow R., Chan T.C., Asashima M.
    Biochem. Biophys. Res. Commun. 295:85-91(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  3. "Nuclear envelope phosphatase-regulatory subunit 1 (formerly TMEM188) is the metazoan SPO7 ortholog and functions in the lipin activation pathway."
    Han S., Bahmanyar S., Zhang P., Grishin N., Oegema K., Crooke R., Graham M., Reue K., Dixon J.E., Goodman J.M.
    J. Biol. Chem. 287:3123-3137(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiCNEP1_CAEEL
AccessioniPrimary (citable) accession number: Q20432
Secondary accession number(s): H1ZUW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.