Q20390 (PPT1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Palmitoyl-protein thioesterase 1
Short name=PPT-1
EC=3.1.2.22

Alternative name(s):
Palmitoyl-protein hydrolase 1

Gene names

Name:	ppt-1
ORF Names:	F44C4.5

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides. Ref.2
Catalytic activity	Palmitoyl-protein + H2O = palmitate + protein.
Disruption phenotype	Worms exhibit a developmental delay and mild reproductive defects. They have abnormal mitochondria in muscle and neuronal cells. Ref.2
Sequence similarities	Belongs to the palmitoyl-protein thioesterase family.
Sequence caution	The sequence CCD71207.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular protein catabolic process Inferred from mutant phenotype Ref.2. Source: WormBase mitochondrion organization Inferred from mutant phenotype Ref.2. Source: WormBase oviposition Inferred from mutant phenotype Ref.2. Source: WormBase post-embryonic development Inferred from mutant phenotype Ref.2. Source: WormBase protein modification process Inferred from electronic annotation. Source: InterPro
Molecular function	palmitoyl-(protein) hydrolase activity Inferred from direct assay Ref.2. Source: WormBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 298	282	Palmitoyl-protein thioesterase 1		PRO_0000247504
Regions
	Compositional bias	197 – 203	7	Poly-Asn
Sites
	Active site	107	1	Nucleophile By similarity
	Active site	224	1	By similarity
	Active site	280	1	By similarity
Amino acid modifications
	Glycosylation	20	1	N-linked (GlcNAc...) Potential
	Glycosylation	250	1	N-linked (GlcNAc...) Potential
	Disulfide bond	37 ↔ 38		By similarity
	Disulfide bond	88 ↔ 120		By similarity
	Disulfide bond	144 ↔ 151		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q20390 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: 31DEFEFB164A6307	FASTA	298	33,984
	10 20 30 40 50 60 MRYFPLLLCL LAITTAEFRN ATKQVPVVMW HGMGDCCCNP LSMGSVKKLF EEQIPGVYVH 70 80 90 100 110 120 SLQLGSSITK DIEHGFYANT NELVYMACIK IKNDPELKNG YNAIGFSQGA QFLRAVAQRC 130 140 150 160 170 180 PNPPMKNLVS VGGQHQGVFG APYCIGDNIM CNGVRRLIDL GAYLPFVQKR VVQAQYWHDP 190 200 210 220 230 240 NQVEEYKKRS IFLADINNEN NNNPTYKRNL LSLKNLVLVK FNQDHMVVPK DSSWFGFYKD 250 260 270 280 290 GDIDTILPMN ETDLYKEDRI GLKKLHESGR IHFMDVDGDH LQIPRSVLVN DIIKKYFM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Identification and characterization of Caenorhabditis elegans palmitoyl protein thioesterase1." Porter M.Y., Turmaine M., Mole S.E. J. Neurosci. Res. 79:836-848(2005) [PubMed: 15672447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-282, FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO081381 Genomic DNA. Translation: CCD71207.1. Different initiation. AY691522 mRNA. Translation: AAU01161.1.
PIR	T30106.
RefSeq	NP_504684.1. NM_072283.4.
UniGene	Cel.2928.
3D structure databases
ProteinModelPortal	Q20390.
SMR	Q20390. Positions 21-297.
ModBase	Search...
Protein-protein interaction databases
STRING	Q20390.
Proteomic databases
PRIDE	Q20390.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	191744.
KEGG	cel:F44C4.5.
NMPDR	fig|6239.3.peg.18203.
UCSC	F44C4.5. c. elegans.
Organism-specific databases
CTD	191744.
WormBase	F44C4.5; CE07253; WBGene00004092; ppt-1.
Phylogenomic databases
eggNOG	meNOG04737.
GeneTree	EMGT00050000016712.
HOGENOM	HBG444329.
InParanoid	Q20390.
PhylomeDB	Q20390.
Gene expression databases
ArrayExpress	Q20390.
Family and domain databases
InterPro	IPR002472. Palm_thioest. [Graphical view]
KO	K01074.
Pfam	PF02089. Palm_thioest. 1 hit. [Graphical view]
PRINTS	PR00414. PPTHIESTRASE.
ProtoNet	Search...

Entry information

Entry name

PPT1_CAEEL

Accession

Primary (citable) accession number: Q20390
Secondary accession number(s): Q68GX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	December 14, 2011
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project