Reviewed, UniProtKB/Swiss-Prot Q20347 (MKK4_CAEEL)

Names and origin

Protein names

Recommended name:
    MAP kinase kinase mkk-4
    EC=2.7.12.2

Gene names

Name:	mkk-4
ORF Names:	F42G10.2

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Activity is required in presynaptic neurons, in a dose-dependent manner, for normal presynaptic development and morphology. Ref.2
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm Ref.2.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
jnk-1	Q8WQG9	1	EBI-321815,EBI-321822
kel-8	O61899	1	EBI-321815,EBI-312469
kgb-1	O44408	1	EBI-321815,EBI-319489

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	MAP kinase kinase mkk-4		PRO_0000086842
Regions
	Domain	66 – 330	265	Protein kinase
	Nucleotide binding	72 – 80	9	ATP By similarity
Sites
	Active site	194	1	Proton acceptor By similarity
	Binding site	95	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q20347-1 [UniParc]. Last modified July 15, 1998. Version 3. Checksum: 643A26A23329759A	FASTA	363	41,197
	10 20 30 40 50 60 MVQEDDENLR NSMSLRPTSL STRPTSLSVN GNEKTLPEES VLRSLSTGTL KYPDDEHLYT 70 80 90 100 110 120 FSSANLQDLG AIGNGNFGTV YKMRHKETGK LIAVKRIRCN NIGHREQIRL LREHDTIVKS 130 140 150 160 170 180 EKGPNIVKFY GAIFSEGDCW ICMELMDISM DLLYKRVYMV KNSRLNENVV GHITVCTVDA 190 200 210 220 230 240 LDYLKKELKI IHRDVKPSNI LVDGTGAVKL CDFGICGQLE ESFAKTHDAG CQPYLAPERI 250 260 270 280 290 300 TSSDKYDVRS DVWSLGITLY EIATGKFPYQ EWNSLFDQIA TVVSGDPPIL HPDSDDFHYS 310 320 330 340 350 360 LPLVKFINTC LTKDRRHRPK YDTLKSFDFY RIYAVAGPEI EEAKRILGVE AIDTRNHPVD HRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Regulation of a DLK-1 and p38 MAP kinase pathway by the ubiquitin ligase RPM-1 is required for presynaptic development." Nakata K., Abrams B., Grill B., Goncharov A., Huang X., Chisholm A.D., Jin Y. Cell 120:407-420(2005) [PubMed: 15707898] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z95122, Z48230 Genomic DNA. Translation: CAB08355.1. Z48230, Z95122 Genomic DNA. Translation: CAA88264.1.
PIR	T22107.
RefSeq	NP_509682.1.
UniGene	Cel.1019
3D structure databases
SMR	Q20347. Positions 64-331.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-25523N.
IntAct	Q20347. 6 interactions.
STRING	Q20347.
Genome annotation databases
Ensembl	F42G10.2; F42G10.2; F42G10.2; Caenorhabditis elegans. [Genome view]
GeneID	185682.
KEGG	cel:F42G10.2.
NMPDR	fig|6239.3.peg.24349.
UCSC	F42G10.2. c. elegans.
Organism-specific databases
CTD	185682.
WormBase	WBGene00003368. mkk-4.
WormPep	F42G10.2. CE10328. [WorfDB]
Phylogenomic databases
eggNOG	meNOG06028.
HOGENOM	HBG755340.
InParanoid	Q20347.
OMA	RITSSDK.
PhylomeDB	Q20347.
Enzyme and pathway databases
BRENDA	2.7.12.2. 672.
Gene expression databases
ArrayExpress	Q20347.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	929126.

Entry information

Entry name

MKK4_CAEEL

Accession

Primary (citable) accession number: Q20347
Secondary accession number(s): O01707

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	January 19, 2010
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project