Reviewed, UniProtKB/Swiss-Prot Q20191 (NAS13_CAEEL)

Names and origin

Protein names

Recommended name:
    Zinc metalloproteinase nas-13
    EC=3.4.24.21
Alternative name(s):
    Nematode astacin 13

Gene names

Name:	nas-13
ORF Names:	F39D8.4

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable metalloprotease By similarity.
Catalytic activity	Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the peptidase M12A family. Contains 2 SXC (ShKT) domains.
Sequence caution	The sequence CAD99208.1 differs from that shown. Reason: Miscellaneous discrepancy. Deletion of several nucleotides that changes the frame.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 450	419	Zinc metalloproteinase nas-13		PRO_0000028917
Regions
	Domain	368 – 405	38	SXC 1
	Domain	414 – 450	37	SXC 2
	Motif	349 – 351	3	Cell attachment site Potential
Sites
	Active site	202	1	By similarity
	Metal binding	201	1	Zinc; catalytic By similarity
	Metal binding	205	1	Zinc; catalytic By similarity
	Metal binding	211	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	68	1	N-linked (GlcNAc...) Potential
	Glycosylation	225	1	N-linked (GlcNAc...) Potential
	Glycosylation	431	1	N-linked (GlcNAc...) Potential
	Disulfide bond	368 ↔ 404		By similarity
	Disulfide bond	375 ↔ 397		By similarity
	Disulfide bond	384 ↔ 401		By similarity
	Disulfide bond	414 ↔ 450		By similarity
	Disulfide bond	421 ↔ 443		By similarity
	Disulfide bond	430 ↔ 447		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q20191-1 [UniParc]. Last modified January 20, 2009. Version 5. Checksum: EBEC8772AD008F06	FASTA	450	50,760
	10 20 30 40 50 60 MPSPTSSSAS VFSSHLFFVF CIFSQIAQSY AQFNRFFDPS IPEQETVLTD YDYNMIGHFR 70 80 90 100 110 120 ENDQWFVNDS AMYNPLRFEG DIANSGLNSR SINTFFGDSP LFGIFGVQRN AVRQTYLKWE 130 140 150 160 170 180 QARIPYTISS QYSSYSRSKI AEAIEEYRKK TCIDFSPKSA GDLDYIHIVP DDGCYSLVGR 190 200 210 220 230 240 IGGKQPVSLG DGCIQKGIII HELMHAVGFF HEQSRADRDE YVKINWSNVE AGLQDQFDKY 250 260 270 280 290 300 SLNMIDHLGT KYDYGSVMHY APTAFSKNGK PTIEPIEKNV EIGQRAGFSE NDIYKINMLY 310 320 330 340 350 360 NCPTFTATTL APENTKRVKS ITKKVTSATG SPLSKKGEIG SSIGDKGDRG DNSVLSSLIS 370 380 390 400 410 420 IHSGLGKCED RRKDCEFLAR AGHCESRFSI RFMTENCANS CGKCIAEEKR KEVCEDARTW 430 440 450 CERWANSGMC NQTVFKDYMR QKCAKSCNFC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"The astacin protein family in Caenorhabditis elegans." Moehrlen F., Hutter H., Zwilling R. Eur. J. Biochem. 270:4909-4920(2003) [PubMed: 14653817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-71, NOMENCLATURE. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z69791, Z69793 Genomic DNA. Translation: CAA93663.4. Z69793, Z69791 Genomic DNA. Translation: CAA93676.4. AJ561206 mRNA. Translation: CAD99208.1. Sequence problems.
PIR	T23864.
UniGene	Cel.1053
3D structure databases
HSSP	HSSP built from PDB template 1IAE based on UniProtKB P07584.
ModBase	Search...
Genome annotation databases
Ensembl	F39D8.4. Caenorhabditis elegans. [Contig view]
NMPDR	fig|6239.3.peg.25424.
Organism-specific databases
WormBase	WBGene00003532. nas-13.
WormPep	F39D8.4. CE43112. [WorfDB]
Enzyme and pathway databases
BRENDA	3.4.24.21. 672.
Gene expression databases
ArrayExpress	Q20191.
Family and domain databases
InterPro	IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR001506. Peptidase_M12A. IPR003582. ShK_toxin. [Graphical view]
Pfam	PF01400. Astacin. 1 hit. PF01549. ShK. 2 hits. [Graphical view]
PRINTS	PR00480. ASTACIN.
SMART	SM00254. ShKT. 2 hits. SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	928468.

Entry information

Entry name

NAS13_CAEEL

Accession

Primary (citable) accession number: Q20191
Secondary accession number(s): Q21661, Q7Z0N3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	January 20, 2009
Last modified:	June 16, 2009
This is version 64 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project