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Reviewed, UniProtKB/Swiss-Prot Q20176 (NAS39_CAEEL)

Last modified June 16, 2009. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Zinc metalloproteinase nas-39
    EC=3.4.24.21
Alternative name(s):
    Nematode astacin 39
Gene names
Name: nas-39
ORF Names: F38E9.2
OrganismCaenorhabditis elegans [Complete proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length951 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Probable metalloprotease By similarity.

Catalytic activity

Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Secreted Potential.

Sequence similarities

Belongs to the peptidase M12A family.

Contains 5 CUB domains.

Contains 2 EGF-like domains.

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Ontologies

Keywords
   Cellular componentSecreted
   DomainEGF-like domain
Repeat
Signal
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Potential
Chain31 – 951921Zinc metalloproteinase nas-39
PRO_0000028943

Regions

Domain249 – 381133CUB 1
Domain382 – 499118CUB 2
Domain499 – 53941EGF-like 1; calcium-binding Potential
Domain542 – 648107CUB 3
Domain648 – 68841EGF-like 2; calcium-binding Potential
Domain692 – 804113CUB 4
Domain805 – 923119CUB 5

Sites

Active site1421 By similarity
Metal binding1411Zinc; catalytic By similarity
Metal binding1451Zinc; catalytic By similarity
Metal binding1511Zinc; catalytic By similarity

Amino acid modifications

Glycosylation691N-linked (GlcNAc...) Potential
Glycosylation871N-linked (GlcNAc...) Potential
Glycosylation2831N-linked (GlcNAc...) Potential
Glycosylation3171N-linked (GlcNAc...) Potential
Glycosylation5501N-linked (GlcNAc...) Potential
Glycosylation5831N-linked (GlcNAc...) Potential
Glycosylation7171N-linked (GlcNAc...) Potential
Disulfide bond249 ↔ 268 By similarity
Disulfide bond382 ↔ 408 By similarity
Disulfide bond435 ↔ 462 By similarity
Disulfide bond503 ↔ 514 By similarity
Disulfide bond510 ↔ 523 By similarity
Disulfide bond525 ↔ 538 By similarity
Disulfide bond542 ↔ 568 By similarity
Disulfide bond596 ↔ 610 By similarity
Disulfide bond652 ↔ 663 By similarity
Disulfide bond659 ↔ 672 By similarity
Disulfide bond674 ↔ 687 By similarity
Disulfide bond692 ↔ 718 By similarity
Disulfide bond745 ↔ 767 By similarity
Disulfide bond805 ↔ 835 By similarity
Disulfide bond863 ↔ 886 By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q20176-1 [UniParc].

Last modified October 1, 2002. Version 3.
Checksum: B5D2A0B258163613

FASTA951107,534
        10         20         30         40         50         60 
MRFSANIAII VNIIFLFIVV EFVLPTFIRS GDVRFRRYYR NNGRVSRAAT AKKERIWPEG 

        70         80         90        100        110        120 
IIPFVIASNF SGEHQHLFLR AMRHWENFTC VSFVPRQPHH KHYITFTVDK CGCCSYVGRR 

       130        140        150        160        170        180 
GEGPQAISIG KNCDKFGIVV HELGHVVGFW HEHTRPDRDM YVDIFYKSIQ TGQDYNFEKS 

       190        200        210        220        230        240 
KPEEVDSLGE PYDFSSIMHY ARDTFSRGAF YDTILPKPNS GFRLEIGQRV QLSEGDIRQT 

       250        260        270        280        290        300 
KKLYKCAECG GTLMQESGNL AIQHAGVCTW HIISPQGHTI FLNITGGLKL IIMIIFLTDE 

       310        320        330        340        350        360 
KLEDLTEIFK PNILKKNQTY LHWKTFLIYY TNSFFHFEIL DRICGGDSLF RTIASSGNRM 

       370        380        390        400        410        420 
LIQVRSSTPA ASLPFATYYA ICGGPIYANE GVIHSPKYPE SYPPNSDCQW TIHVDENSQV 

       430        440        450        460        470        480 
AIEFVYFHLE QHKECIYDRL ILTEGISKNS KKDGKEMSET FCGLIEKKTI VSKTNQISLR 

       490        500        510        520        530        540 
FFSDNSVQKT GFELRFTKEL NECATDKNIC HHYCVNTVGG FKCACRVGYS LSSNGFSCDS 

       550        560        570        580        590        600 
TCGGYLKASN GSISSPNFPE MYPNSKTCIW EIEAPDGYHI FLNFTKFNVE GMKTECAYDY 

       610        620        630        640        650        660 
VKIGDSEKLC GEYHEALLFT TPRNRVRIEF SSDSSVERDG FFANFIADFD ECQNDNAGCE 

       670        680        690        700        710        720 
HTCQNRLGSY VCTCNPGYIL AEDKHNCKEG SCFFEVNAPA GDINSPNYPN DYPKGQNCSW 

       730        740        750        760        770        780 
HFVTTPGHRL MLTFSSFQVE EHAQCKYDAV SVYDGGDGSA QLAGVFCGLA PPPLLLSSSN 

       790        800        810        820        830        840 
ELYLTFSSDA SVSRRGFQAH YTSLCGGRLT AESTPGHIYS HATFSDSKYG KNQDCSWIVR 

       850        860        870        880        890        900 
AKSPGRGVRI QFSTFNIESE EGCQYDYIEI YDGPEATLER LVGRFCGDTS PEVITSTGPE 

       910        920        930        940        950 
LLLIMHTDNA EEEKGFVAEY REAPRSSSTK RTFVSKTRHS PLEEPIHDRN E

« Hide

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References

« Hide 'large scale' references
[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[2]"The astacin protein family in Caenorhabditis elegans."
Moehrlen F., Hutter H., Zwilling R.
Eur. J. Biochem. 270:4909-4920(2003) [PubMed: 14653817] [Abstract]
Cited for: IDENTIFICATION, NOMENCLATURE.

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Cross-references

Sequence databases

U46668 Genomic DNA. Translation: AAA93348.3.
PIRT30018.
RefSeqNP_510672.2.
UniGeneCel.8940

3D structure databases

HSSPHSSP built from PDB template 1NZI based on UniProtKB Q9UCV4.
ModBaseSearch...

Genome annotation databases

EnsemblF38E9.2. Caenorhabditis elegans. [Contig view]
GeneID3565986.
KEGGcel:F38E9.2.
NMPDRfig|6239.3.peg.25588.

Organism-specific databases

WormBaseWBGene00003555. nas-39.
WormPepF38E9.2. CE30977. [WorfDB]

Phylogenomic databases

OMAQ20176. FRTIASS.

Enzyme and pathway databases

BRENDA3.4.24.21. 672.

Gene expression databases

ArrayExpressQ20176.

Family and domain databases

InterProIPR015446. BMP_1/tolloid-like.
IPR000859. CUB.
IPR006209. EGF.
IPR013032. EGF-like_reg_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_CS.
IPR000742. EGF_3.
IPR001881. EGF_Ca_bd.
IPR013091. EGF_Ca_bd_2.
IPR018097. EGF_Ca_bd_CS.
IPR006025. Pept_M_Zn_BS.
IPR006026. Peptidase_M.
IPR001506. Peptidase_M12A.
[Graphical view]
Gene3DG3DSA:2.60.120.290. CUB. 4 hits.
PfamPF01400. Astacin. 1 hit.
PF00431. CUB. 4 hits.
PF00008. EGF. 1 hit.
PF07645. EGF_CA. 1 hit.
[Graphical view]
PIRSFPIRSF001199. BMP_1/tolloid-like. 1 hit.
PRINTSPR00480. ASTACIN.
SMARTSM00042. CUB. 5 hits.
SM00179. EGF_CA. 2 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEPS00010. ASX_HYDROXYL. 2 hits.
PS01180. CUB. 5 hits.
PS00022. EGF_1. False negative.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio958633.

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Entry information

Entry nameNAS39_CAEEL
AccessionPrimary (citable) accession number: Q20176
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 1, 2002
Last modified: June 16, 2009
This is version 64 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectCaenorhabditis annotation project

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Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents