Q20117 (GSH1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--cysteine ligase
EC=6.3.2.2

Alternative name(s):
Gamma-ECS
Short name=GCS
Gamma-glutamylcysteine synthetase

Gene names

Name:	gcs-1
ORF Names:	F37B12.2

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	654 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
Sequence similarities	Belongs to the glutamate--cysteine ligase type 3 family.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from mutant phenotype. Source: WormBase response to arsenic-containing substance Inferred from mutant phenotype. Source: WormBase response to heat Inferred from expression pattern. Source: WormBase response to herbicide Inferred from expression pattern. Source: WormBase
Cellular component	cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Inferred from Biological aspect of Ancestor. Source: RefGenome protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
E01A2.1	Q966L2	2	EBI-311822,EBI-311817

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 654	654	Glutamate--cysteine ligase		PRO_0000192566

Sequences

	Sequence		Length	Mass (Da)
	Q20117 [UniParc]. Last modified April 11, 2003. Version 2. Checksum: 37EA2F5DFC2BEAB5	FASTA	654	74,369
	10 20 30 40 50 60 MGLLTKGSPL TWAETVPHID YIKKHGIAQF INLYHRLKSR HGDQLKWGDE IEYTIVKFDD 70 80 90 100 110 120 ANKKVRVSCK AEELLNKLQA EEQVNAMLGT ANRFLWRPEF GSYMIEGTPG MPYGGLIACF 130 140 150 160 170 180 NIVEANMKLR RQVVKKLLKK DETCLSISFP SLGVPGFTFP EVAADRKNDD AANSVFWPEQ 190 200 210 220 230 240 AVFLGHPRFK NLTKNIKGRR GSKVAINVPI FKDTNTPSPF VEDLSALGGP DDTRDAKPDH 250 260 270 280 290 300 IYMDHMGFGM GCCCLQVTFQ AVNVDEARWL YDQLTPITPI LLALSAATPI FRGKLSNVDS 310 320 330 340 350 360 RWDIISASVD DRTPEERGLE PLKNSKWVID KSRYDSTDCY IYPCSVGYND IPLQYDETIY 370 380 390 400 410 420 KQLIDGNIDE PLAKHIAHMF IRDPHQVFRE RIEQDDEKSS EHFETIQSSN WMNMRFKPPP 430 440 450 460 470 480 PDAPEIGWRV EFRPTEVQLT DFENAAYCCF VVLLTRMMIS FRLTYLMPIS MVTENMKRAQ 490 500 510 520 530 540 QKDAVLNQKF LFRKGLAECK SAPENLKGSE KCGPPSQDIE EMSIDEIING KKNGFPGLIS 550 560 570 580 590 600 LIRQFLDSAD VDVDTRCTIS QYLNFISKRA TGEINTLAHW TRGFVQSHPA YKHDSDVNDN 610 620 630 640 650 IVYDLLKKMD AISNGEDHCE KLLGCYRSKT DHAISAAVRK AEEHMIVSSQ KRAH

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z54218 Genomic DNA. Translation: CAA90955.2.
PIR	T21908.
RefSeq	NP_495927.2. NM_063526.4.
UniGene	Cel.17240.
3D structure databases
ProteinModelPortal	Q20117.
SMR	Q20117. Positions 2-636.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q20117. 1 interaction.
MINT	MINT-1060220.
STRING	Q20117.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F37B12.2.1; F37B12.2.1; F37B12.2. F37B12.2.2; F37B12.2.2; F37B12.2.
GeneID	174438.
KEGG	cel:F37B12.2.
UCSC	F37B12.2.1. c. elegans.
Organism-specific databases
CTD	174438.
WormBase	F37B12.2; CE32886; WBGene00001527; gcs-1.
Phylogenomic databases
eggNOG	meNOG04688.
GeneTree	EMGT00050000016273.
HOGENOM	HBG331200.
InParanoid	Q20117.
OMA	QVTFQAC.
PhylomeDB	Q20117.
Gene expression databases
ArrayExpress	Q20117.
Family and domain databases
InterPro	IPR004308. GCS. [Graphical view]
KO	K11204.
PANTHER	PTHR11164. GCS. 1 hit.
Pfam	PF03074. GCS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	884042.

Entry information

Entry name

GSH1_CAEEL

Accession

Primary (citable) accession number: Q20117

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	April 11, 2003
Last modified:	November 16, 2011
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project