Reviewed, UniProtKB/Swiss-Prot Q20117 (GSH1_CAEEL)

Names and origin

Protein names

Recommended name:
    Glutamate--cysteine ligase
    EC=6.3.2.2
Alternative name(s):
    Gamma-glutamylcysteine synthetase
    Gamma-ECS
      Short name=GCS

Gene names

Name:	gcs-1
ORF Names:	F37B12.2

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	654 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
Sequence similarities	Belongs to the glutamate--cysteine ligase type 3 family.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q966L2	2	EBI-311822,EBI-311817

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 654	654	Glutamate--cysteine ligase		PRO_0000192566

Sequences

	Sequence		Length	Mass (Da)
	Q20117-1 [UniParc]. Last modified April 11, 2003. Version 2. Checksum: 37EA2F5DFC2BEAB5	FASTA	654	74,369
	10 20 30 40 50 60 MGLLTKGSPL TWAETVPHID YIKKHGIAQF INLYHRLKSR HGDQLKWGDE IEYTIVKFDD 70 80 90 100 110 120 ANKKVRVSCK AEELLNKLQA EEQVNAMLGT ANRFLWRPEF GSYMIEGTPG MPYGGLIACF 130 140 150 160 170 180 NIVEANMKLR RQVVKKLLKK DETCLSISFP SLGVPGFTFP EVAADRKNDD AANSVFWPEQ 190 200 210 220 230 240 AVFLGHPRFK NLTKNIKGRR GSKVAINVPI FKDTNTPSPF VEDLSALGGP DDTRDAKPDH 250 260 270 280 290 300 IYMDHMGFGM GCCCLQVTFQ AVNVDEARWL YDQLTPITPI LLALSAATPI FRGKLSNVDS 310 320 330 340 350 360 RWDIISASVD DRTPEERGLE PLKNSKWVID KSRYDSTDCY IYPCSVGYND IPLQYDETIY 370 380 390 400 410 420 KQLIDGNIDE PLAKHIAHMF IRDPHQVFRE RIEQDDEKSS EHFETIQSSN WMNMRFKPPP 430 440 450 460 470 480 PDAPEIGWRV EFRPTEVQLT DFENAAYCCF VVLLTRMMIS FRLTYLMPIS MVTENMKRAQ 490 500 510 520 530 540 QKDAVLNQKF LFRKGLAECK SAPENLKGSE KCGPPSQDIE EMSIDEIING KKNGFPGLIS 550 560 570 580 590 600 LIRQFLDSAD VDVDTRCTIS QYLNFISKRA TGEINTLAHW TRGFVQSHPA YKHDSDVNDN 610 620 630 640 650 IVYDLLKKMD AISNGEDHCE KLLGCYRSKT DHAISAAVRK AEEHMIVSSQ KRAH

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z54218 Genomic DNA. Translation: CAA90955.2.
PIR	T21908.
RefSeq	NP_495927.2.
UniGene	Cel.17240
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q20117. 1 interaction.
Genome annotation databases
Ensembl	F37B12.2. Caenorhabditis elegans. [Contig view]
GeneID	174438.
KEGG	cel:F37B12.2.
Organism-specific databases
WormBase	WBGene00001527. gcs-1.
WormPep	F37B12.2. CE32886. [WorfDB]
Phylogenomic databases
OMA	Q20117. FNTVEDN.
Enzyme and pathway databases
BRENDA	6.3.2.2. 672.
Gene expression databases
ArrayExpress	Q20117.
Family and domain databases
InterPro	IPR004308. GCS. [Graphical view]
PANTHER	PTHR11164. GCS. 1 hit.
Pfam	PF03074. GCS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	884042.

Entry information

Entry name

GSH1_CAEEL

Accession

Primary (citable) accession number: Q20117

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	April 11, 2003
Last modified:	June 16, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project