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Protein

Structural maintenance of chromosomes protein 4

Gene

smc-4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. Also involved in chromosome segregation.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi121 – 1288ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome condensation Source: UniProtKB-KW
  • mitotic sister chromatid segregation Source: WormBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, DNA condensation, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-2299718. Condensation of Prophase Chromosomes.

Names & Taxonomyi

Protein namesi
Recommended name:
Structural maintenance of chromosomes protein 4
Short name:
SMC protein 4
Short name:
SMC-4
Gene namesi
Name:smc-4
ORF Names:F35G12.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiF35G12.8; CE03287; WBGene00004874; smc-4.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication
  • Chromosome 1 Publication

  • Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, condensin associates with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.

GO - Cellular componenti

  • chromosome, centromeric region Source: WormBase
  • condensed chromosome Source: WormBase
  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15491549Structural maintenance of chromosomes protein 4PRO_0000119024Add
BLAST

Proteomic databases

EPDiQ20060.
PaxDbiQ20060.
PRIDEiQ20060.

PTM databases

iPTMnetiQ20060.

Interactioni

Subunit structurei

Forms a heterodimer with mix-1. Component of the condensin complex, which contains the mix-1 and smc4 heterodimer, and probably non smc subunits that regulate the complex.

Binary interactionsi

WithEntry#Exp.IntActNotes
mix-1Q095913EBI-1152127,EBI-1152136

Protein-protein interaction databases

BioGridi40839. 1 interaction.
IntActiQ20060. 3 interactions.
STRINGi6239.F35G12.8.

Structurei

3D structure databases

ProteinModelPortaliQ20060.
SMRiQ20060. Positions 92-307, 604-762, 1055-1355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni605 – 785181Flexible hingeAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili326 – 604279Sequence analysisAdd
BLAST
Coiled coili786 – 1058273Sequence analysisAdd
BLAST
Coiled coili1144 – 118239Sequence analysisAdd
BLAST

Domaini

The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with mix-1, forming a V-shaped heterodimer.By similarity

Sequence similaritiesi

Belongs to the SMC family. SMC4 subfamily.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0996. Eukaryota.
COG1196. LUCA.
GeneTreeiENSGT00580000081617.
HOGENOMiHOG000021360.
InParanoidiQ20060.
KOiK06675.
OMAiNNLRENI.
OrthoDBiEOG7RJPQJ.
PhylomeDBiQ20060.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR027417. P-loop_NTPase.
IPR003395. RecF/RecN/SMC_N.
IPR024704. SMC.
IPR010935. SMC_hinge.
[Graphical view]
PfamiPF06470. SMC_hinge. 1 hit.
PF02463. SMC_N. 1 hit.
[Graphical view]
PIRSFiPIRSF005719. SMC. 1 hit.
SMARTiSM00968. SMC_hinge. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF75553. SSF75553. 1 hit.

Sequencei

Sequence statusi: Complete.

Q20060-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPKTSAAPP QSDESDSDFD DAPVKKPQKK TTKPVNRHKE GSKDPEEELQ
60 70 80 90 100
RAVNEKFDGS DGEDDDSDLF SLQLPSRPDF LTKPNRADRL MIRNVEVDNF
110 120 130 140 150
KSYFGKASIG PFHKSFTSII GPNGSGKSNL IDSLLFVFGF RASKIRSAKV
160 170 180 190 200
SNLIHKSAGR NPDKCTVTIH FQRIVDIPGH YEVVKDSEFT ISRTAFQNNS
210 220 230 240 250
SSYAIDGRPA TKNEVEARLR RVDIDIEHNR FLILQGEVEQ IAMMKPVKTT
260 270 280 290 300
KSETGMVEYL EDIIGTNRLE PFVKLFQRRV NRLTCDLSQQ RIARDHARNS
310 320 330 340 350
KVAMENPVRA AIEFLMKENE ATTIHMKLEQ RRRQRYLDKI APKQAELDKM
360 370 380 390 400
KEEMKSIAET LDTNKNEYKQ SEEAQKVMIE ERSKLDKNFD SLSKELSDLG
410 420 430 440 450
TEETRRKEAL KRHQANISKA EAEKEKEVKK RSNLEAAPEK AERKIAKCQE
460 470 480 490 500
EVEQLLEIEK TANEEADKNL DEFEKRSEAP KEEQKKIQET WAQKSNEFNK
510 520 530 540 550
VRGEARIARE DFEDLKKLAN SGTDKLIELK KRLESSEESY AKEKDELDKL
560 570 580 590 600
KPEFDSWNDK LKQLSTELPT LRNTARQKNQ DLAKTRDRLE TLRQQNSSCS
610 620 630 640 650
SSNKVIQALM KEKEAGRIKS FHGRLGDLGV IDPKYEGAIC TNFGARLNYL
660 670 680 690 700
IVGKEEDAKN VINFLVANKL PRQTVQPLDK IKCDKRDLAP NPTNPLPAPR
710 720 730 740 750
LIDLIDCDPV LKPAFYDMVR SAIVGDSTQE AQRMHRMPAC RGVTVCTLEG
760 770 780 790 800
SMIHPSGSFT GGGKTVKGLI LTDKNKMAKQ VTPEDKAAER DLAEKLGKLR
810 820 830 840 850
DEADELKGQE HEMDGQLIEA RRKVAEMSNR LSIVTSSVQS AAPAIETLKK
860 870 880 890 900
TIANQEKEAA KVKVDAKTLE DKQKIVEELE KKRDELGEEA AKVKARQAEI
910 920 930 940 950
QSKLDGIFKE LVQCHRDEAK ESLQKRQKLE KDIAKETANI SNSGRNIAKC
960 970 980 990 1000
DENISRHDKD IEKMKKKCEE LMEKAIDDEE VKSKKETVER FEKQIKKLQT
1010 1020 1030 1040 1050
KGEEMTKKQS ELSAAETKLE GELKKCSEGI KELKESMLAD RLKVEDIEKK
1060 1070 1080 1090 1100
LAALKVNRIP RFQFLIESSR PEDLEMQIDD KMPVVDENQS PEEVERQKKH
1110 1120 1130 1140 1150
MACVMSDAAY ALEFEMRQKV LENTESYENV DGEDRVPVEL LSDEKINEIS
1160 1170 1180 1190 1200
SRDAEEMQMK LKVCEQQVEA LKAKVDISSI KAYVDKVKQY NEQVIKLTIA
1210 1220 1230 1240 1250
TEVHRKHNQE LQRIKQMRLE EFHSAFEFIG KHLVAVFKML TDGGDAKLEY
1260 1270 1280 1290 1300
IDKDDPFRQG ISFMVRPAKK AWKQIQFLSG GEKTLSSLAL IFALHMFRPT
1310 1320 1330 1340 1350
PFYVMDEIDA ALDYRNVSII AQYVRQKTEN AQFIIISLRN NMFELANRLV
1360 1370 1380 1390 1400
GIYKVDGCTR NVAIDPLRVC EMAKQITDSL GQATCTLPDE VTQRFNETMS
1410 1420 1430 1440 1450
RQNKEMIAQE KQYPNFPSSN EISKAEKIVN VEGRVRKELI QTTRDVTSRP
1460 1470 1480 1490 1500
QSKATTSGDG TERPASRSAS RPESRINQMK YPAPRLVERS SSQNVRSPRK
1510 1520 1530 1540
ARNIEADETT PPSKRSNSAS TPKRSPMKPL TPSSKKKEKA IVDDDDDME
Length:1,549
Mass (Da):176,572
Last modified:November 1, 1996 - v1
Checksum:iDF752147301934BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46242 Genomic DNA. Translation: CAA86336.1.
PIRiT21809.
RefSeqiNP_497935.1. NM_065534.5.
UniGeneiCel.5415.

Genome annotation databases

EnsemblMetazoaiF35G12.8; F35G12.8; WBGene00004874.
GeneIDi175603.
KEGGicel:CELE_F35G12.8.
UCSCiF35G12.8. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46242 Genomic DNA. Translation: CAA86336.1.
PIRiT21809.
RefSeqiNP_497935.1. NM_065534.5.
UniGeneiCel.5415.

3D structure databases

ProteinModelPortaliQ20060.
SMRiQ20060. Positions 92-307, 604-762, 1055-1355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi40839. 1 interaction.
IntActiQ20060. 3 interactions.
STRINGi6239.F35G12.8.

PTM databases

iPTMnetiQ20060.

Proteomic databases

EPDiQ20060.
PaxDbiQ20060.
PRIDEiQ20060.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF35G12.8; F35G12.8; WBGene00004874.
GeneIDi175603.
KEGGicel:CELE_F35G12.8.
UCSCiF35G12.8. c. elegans.

Organism-specific databases

CTDi175603.
WormBaseiF35G12.8; CE03287; WBGene00004874; smc-4.

Phylogenomic databases

eggNOGiKOG0996. Eukaryota.
COG1196. LUCA.
GeneTreeiENSGT00580000081617.
HOGENOMiHOG000021360.
InParanoidiQ20060.
KOiK06675.
OMAiNNLRENI.
OrthoDBiEOG7RJPQJ.
PhylomeDBiQ20060.

Enzyme and pathway databases

ReactomeiR-CEL-2299718. Condensation of Prophase Chromosomes.

Miscellaneous databases

NextBioi888868.
PROiQ20060.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR027417. P-loop_NTPase.
IPR003395. RecF/RecN/SMC_N.
IPR024704. SMC.
IPR010935. SMC_hinge.
[Graphical view]
PfamiPF06470. SMC_hinge. 1 hit.
PF02463. SMC_N. 1 hit.
[Graphical view]
PIRSFiPIRSF005719. SMC. 1 hit.
SMARTiSM00968. SMC_hinge. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF75553. SSF75553. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "C. elegans condensin promotes mitotic chromosome architecture, centromere organization, and sister chromatid segregation during mitosis and meiosis."
    Hagstrom K.A., Holmes V.F., Cozzarelli N.R., Meyer B.J.
    Genes Dev. 16:729-742(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MIX-1.

Entry informationi

Entry nameiSMC4_CAEEL
AccessioniPrimary (citable) accession number: Q20060
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: November 1, 1996
Last modified: March 16, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.