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Q1ZZU7 (MIF_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Macrophage migration inhibitory factor

Short name=MIF
EC=5.3.2.1
Alternative name(s):
L-dopachrome isomerase
L-dopachrome tautomerase
EC=5.3.3.12
Phenylpyruvate tautomerase
Gene names
Name:MIF
OrganismOvis aries (Sheep) [Reference proteome]
Taxonomic identifier9940 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis

Protein attributes

Sequence length115 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity By similarity.

Catalytic activity

Keto-phenylpyruvate = enol-phenylpyruvate.

L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.

Subunit structure

Homotrimer. Interacts with BNIPL By similarity. Interacts with the CD74 extracellular domain. Interacts with COPS5 and with the CXCR2 extracellular domain By similarity.

Subcellular location

Secreted By similarity. Cytoplasm By similarity. Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens By similarity.

Sequence similarities

Belongs to the MIF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 115114Macrophage migration inhibitory factor
PRO_0000344369

Sites

Active site21Proton acceptor; via imino nitrogen By similarity
Binding site331Substrate By similarity
Binding site651Substrate; via amide nitrogen By similarity
Binding site981Substrate By similarity

Amino acid modifications

Modified residue781N6-acetyllysine; alternate By similarity
Modified residue781N6-succinyllysine; alternate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1ZZU7 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: 750242BFF691975E

FASTA11512,343
        10         20         30         40         50         60 
MPMFVVNTNV PRASVPDGLL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF GGSSEPCALC 

        70         80         90        100        110 
SLHSIGKIGG AQNRSYSKLL CGLLTERLRI SPDRIYINFC DMNAANVGWN GSTFA 

« Hide

References

[1]"The entire coding sequence of sheep macrophage migration inhibitory factor."
Lopes F., Ptak G., Arcuri F.
Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Placenta.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ414692 mRNA. Translation: ABD67167.1.
RefSeqNP_001072123.1. NM_001078655.1.
UniGeneOar.4767.

3D structure databases

ProteinModelPortalQ1ZZU7.
SMRQ1ZZU7. Positions 1-115.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ1ZZU7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID780466.

Organism-specific databases

CTD4282.

Phylogenomic databases

HOVERGENHBG003240.

Family and domain databases

InterProIPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERPTHR11954. PTHR11954. 1 hit.
PfamPF01187. MIF. 1 hit.
[Graphical view]
ProDomPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF55331. SSF55331. 1 hit.
PROSITEPS01158. MIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMIF_SHEEP
AccessionPrimary (citable) accession number: Q1ZZU7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: April 18, 2006
Last modified: July 9, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families