Q1ZZU7 (MIF_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Macrophage migration inhibitory factor
Short name=MIF
EC=5.3.2.1

Alternative name(s):
L-dopachrome isomerase
L-dopachrome tautomerase
EC=5.3.3.12
Phenylpyruvate tautomerase

Gene names

Name:

MIF

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	115 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity By similarity.
Catalytic activity	Keto-phenylpyruvate = enol-phenylpyruvate. L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.
Subunit structure	Homotrimer. Interacts with BNIPL By similarity. Interacts with the CD74 extracellular domain. Interacts with COPS5 and with the CXCR2 extracellular domain By similarity.
Subcellular location	Secreted By similarity. Cytoplasm By similarity. Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens By similarity.
Sequence similarities	Belongs to the MIF family.

Ontologies

Keywords
Biological process	Immunity Inflammatory response Innate immunity
Cellular component	Cytoplasm Secreted
Molecular function	Cytokine Isomerase
PTM	Acetylation
Gene Ontology (GO)
Biological_process	inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	dopachrome isomerase activity Inferred from sequence or structural similarity. Source: UniProtKB phenylpyruvate tautomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 115	114	Macrophage migration inhibitory factor		PRO_0000344369
Sites
	Active site	2	1	Proton acceptor; via imino nitrogen By similarity
	Binding site	33	1	Substrate By similarity
	Binding site	65	1	Substrate; via amide nitrogen By similarity
	Binding site	98	1	Substrate By similarity
Amino acid modifications
	Modified residue	78	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1ZZU7 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 750242BFF691975E	FASTA	115	12,343
	10 20 30 40 50 60 MPMFVVNTNV PRASVPDGLL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF GGSSEPCALC 70 80 90 100 110 SLHSIGKIGG AQNRSYSKLL CGLLTERLRI SPDRIYINFC DMNAANVGWN GSTFA

References

[1]	"The entire coding sequence of sheep macrophage migration inhibitory factor." Lopes F., Ptak G., Arcuri F. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ414692 mRNA. Translation: ABD67167.1.
RefSeq	NP_001072123.1. NM_001078655.1.
UniGene	Oar.4767.
3D structure databases
ProteinModelPortal	Q1ZZU7.
SMR	Q1ZZU7. Positions 1-115.
ModBase	Search...
Proteomic databases
PRIDE	Q1ZZU7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	780466.
Organism-specific databases
CTD	4282.
Phylogenomic databases
HOVERGEN	HBG003240.
Family and domain databases
InterPro	IPR001398. Macrophage_inhib_fac. IPR019829. Macrophage_inhib_fac_CS. IPR014347. Tautomerase/MIF_sf. [Graphical view]
PANTHER	PTHR11954. PTHR11954. 1 hit.
Pfam	PF01187. MIF. 1 hit. [Graphical view]
ProDom	PD004816. Macrophage_inhib_fac. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF55331. SSF55331. 1 hit.
PROSITE	PS01158. MIF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MIF_SHEEP

Accession

Primary (citable) accession number: Q1ZZU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	April 18, 2006
Last modified:	March 6, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program