Reviewed, UniProtKB/Swiss-Prot Q1ZXQ5 (FAD5C_DICDI)

Names and origin

Protein names

Recommended name:
    Probable Delta(5) fatty acid desaturase C
      Short name=Delta-5 fatty acid desaturase C
    EC=1.14.19.-

Gene names

ORF Names:

DDB_G0294553

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Cofactor	Iron By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Domain	The histidine box domains may contain the active site and/or be involved in metal ion binding By similarity.
Sequence similarities	Belongs to the fatty acid desaturase family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Fatty acid biosynthesis Lipid synthesis Transport
Cellular component	Membrane
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Probable Delta(5) fatty acid desaturase C		PRO_0000327537
Regions
	Transmembrane	121 – 141	21	Potential
	Transmembrane	151 – 171	21	Potential
	Transmembrane	187 – 207	21	Potential
	Transmembrane	289 – 309	21	Potential
	Transmembrane	315 – 335	21	Potential
	Domain	9 – 87	79	Cytochrome b5 heme-binding
	Motif	174 – 178	5	Histidine box-1
	Motif	210 – 215	6	Histidine box-2
	Motif	394 – 398	5	Histidine box-3
Sites
	Metal binding	45	1	Iron (heme axial ligand) By similarity
	Metal binding	68	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1ZXQ5-1 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: FCF766B733B98A66	FASTA	459	53,864
	10 20 30 40 50 60 MENEKKLFKK LYSWKEISKH NTIENGIWIS IDGLVYDITK FIKHHPGGEQ VLILAAGRDV 70 80 90 100 110 120 TNLFESYHPM TDLPSKMLKQ YEIGQVSTME FPKYVEKSKF YSTLKERVRE HFKKSNKDPK 130 140 150 160 170 180 FAFGIIARLI FVYWFLITSY YVSHYAFIEN FYLNCLLAIV YSLSNSLFSL HMMHDACHSA 190 200 210 220 230 240 ISHNPKVWKW LGATYDLFIG ASFFYWCNQH VIGHHVYTNI RNADPDIGDS EVDFRIVTPY 250 260 270 280 290 300 QNKYWIYKYQ HIYAPFLYGL YSIKYRLCDY SVFTEGSIGR VRTANASNFE IISFIIGKLV 310 320 330 340 350 360 FIVFRFIIPL QYHSLVNLLT YFFIAEFFFG LYLSFGFQVS HSADNLKIVA TSVNENDEPT 370 380 390 400 410 420 NVDEDWAIHQ IKTTQDYGIN SYMCLFFSGG VNLQVVHHLF PSISQEYYGE LVPIIKKVCD 430 440 450 EYDVHYNIQP TFYAAFKSHI DFLYNMGNNE NYVRKSVTD

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000005 Genomic DNA. Translation: EAS66933.1.
RefSeq	XP_001134469.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4238026.
KEGG	ddi:DDB_0231853.
Organism-specific databases
dictyBase	DDB_G0294553.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. IPR012171. Fatty_acid/sphinglp_desaturase. IPR005804. Fatty_acid_desaturase-1. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. PF00487. FA_desaturase. 1 hit. [Graphical view]
PIRSF	PIRSF015921. FA_sphinglp_des. 1 hit.
PRINTS	PR00363. CYTOCHROMEB5.
ProDom	PD000612. Cyt_B5. 1 hit. PD001081. FA_desat_sub. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FAD5C_DICDI

Accession

Primary (citable) accession number: Q1ZXQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 2, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)