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Protein

Alpha-mannosidase E

Gene

manE

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi67ZincBy similarity1
Metal bindingi69ZincBy similarity1
Active sitei150NucleophileBy similarity1
Metal bindingi150ZincBy similarity1
Metal bindingi409ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DDI-975578. Reactions specific to the complex N-glycan synthesis pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase E (EC:3.2.1.24)
Gene namesi
Name:manE
ORF Names:DDB_G0278651
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278651. manE.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 1072ExtracellularSequence analysisAdd BLAST1051
Transmembranei1073 – 1093HelicalSequence analysisAdd BLAST21
Topological domaini1094 – 1123CytoplasmicSequence analysisAdd BLAST30

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000032784422 – 1123Alpha-mannosidase EAdd BLAST1102

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi2N-linked (GlcNAc...)Sequence analysis1
Glycosylationi38N-linked (GlcNAc...)Sequence analysis1
Glycosylationi140N-linked (GlcNAc...)Sequence analysis1
Glycosylationi521N-linked (GlcNAc...)Sequence analysis1
Glycosylationi675N-linked (GlcNAc...)Sequence analysis1
Glycosylationi858N-linked (GlcNAc...)Sequence analysis1
Glycosylationi887N-linked (GlcNAc...)Sequence analysis1
Glycosylationi975N-linked (GlcNAc...)Sequence analysis1
Glycosylationi990N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ1ZXI8.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0231615.

Structurei

3D structure databases

ProteinModelPortaliQ1ZXI8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi451 – 456Poly-Asn6
Compositional biasi1020 – 1023Poly-Asn4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1958. Eukaryota.
ENOG410XQMZ. LUCA.
InParanoidiQ1ZXI8.
OMAiSINEIMA.
PhylomeDBiQ1ZXI8.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 2 hits.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
IPR013780. Glyco_hydro_b.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 2 hits.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 2 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q1ZXI8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKTKLIKII FVIGVWILLS TFIINIYNEN FEIVNYNNNS QSLKKIFQLN
60 70 80 90 100
GDLKDEKLSI FLIPHSHCDS GWLQDYDWYY NHVVQYILSG IVNELNLDKE
110 120 130 140 150
KKFNWVEIGG WVQNDEATAT IDDVITQMTQ GHQWLKDTLN YTIEYAWQID
160 170 180 190 200
PFGYSSSTPT IFSSMGIKGL IINRVSDDVK SYMKSAKEME FIWKGSESLG
210 220 230 240 250
EQSQMLVSTL NVHYDYPKHI DPKKDFSLEE RVKGFTKYIK DLSNTRESSI
260 270 280 290 300
LMIPLGDDFR YSNAKNEFSV SKEWLKVIQD NKEKYNIKEI KYATIDEYFI
310 320 330 340 350
ALEDEFMNKL GKTVEENLKT GFSSTLSLYN KDFFPYSTGD LEYWTGYYTT
360 370 380 390 400
RPLLKRLIRE SSLLQKSSDI LYTLAIGENS NNQIDINNLQ TLSNQLNENR
410 420 430 440 450
NTIALVQHHD IVTGTSRSFV LNDNFQRLQK SRISNYNIIS NSLEYLLNKD
460 470 480 490 500
NNNNENTDST NEPFNFENVI DLSNEINNQY SLVFHNTLGW EVNQHVSFRI
510 520 530 540 550
KVNKNDNQLL ESIQLVEAIS NKTIQIQIIP IQDDSNCQSI ENNYIVFAII
560 570 580 590 600
NLPPLGLNTY YLSISSNEDS KPNTILSKPK LLKKGNEINF NNNRFKVEFE
610 620 630 640 650
SNGLISKITD KNSNEIKTIE QTFHQYSTKK SGPYIFNVKG GKKHGFLENP
660 670 680 690 700
DKFIYHDGPL VSQLTMLYGV DDGCNVTSIV VQRIYKNNEI DNSNSKSLIT
710 720 730 740 750
ENYIETGYSI NGDMNRETTI NYKVKDLEND DIFYTDNGLE SRKRIYNHDR
760 770 780 790 800
TVNQNYYPVL GYIKLKETSE NNNHQYTVYV DRSVGATSPS DGEMEIMIHR
810 820 830 840 850
TMDTDDWKGV NWPSKDIGRS DAKLYFNMDL VNNQLQNEKR ISLHITNQPI
860 870 880 890 900
MFVKKHNNQT GYLLKYSPLS NQLPSNIHLQ SLLTLKNNTV GLRLFNIHEV
910 920 930 940 950
DSNTQSTTDT DKSTLFNELE ISNFIETGLS FLKLTKNNLI DKYSTRINKK
960 970 980 990 1000
FPVKCGESEY SFINEKPSSI IFSNNGTNNI IDGENKGENN KTIETIQIKP
1010 1020 1030 1040 1050
HEIKSFTFNF NFQLDQIIPN NNNYAINKEL NNEYIEQSIE NQRYEYDFSF
1060 1070 1080 1090 1100
FPIKPTFYDD RGKYNRPNHL ALILSLSIGT PAGILIIVIA LVVIYKKRKN
1110 1120
RKTLTSSYSL LNLILKDRAD SSP
Length:1,123
Mass (Da):129,935
Last modified:May 2, 2006 - v1
Checksum:i7EAB06BD0CC3966C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000023 Genomic DNA. Translation: EAS66899.1.
RefSeqiXP_001134583.1. XM_001134583.1.

Genome annotation databases

EnsemblProtistsiEAS66899; EAS66899; DDB_G0278651.
GeneIDi8621484.
KEGGiddi:DDB_G0278651.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000023 Genomic DNA. Translation: EAS66899.1.
RefSeqiXP_001134583.1. XM_001134583.1.

3D structure databases

ProteinModelPortaliQ1ZXI8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0231615.

Proteomic databases

PaxDbiQ1ZXI8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAS66899; EAS66899; DDB_G0278651.
GeneIDi8621484.
KEGGiddi:DDB_G0278651.

Organism-specific databases

dictyBaseiDDB_G0278651. manE.

Phylogenomic databases

eggNOGiKOG1958. Eukaryota.
ENOG410XQMZ. LUCA.
InParanoidiQ1ZXI8.
OMAiSINEIMA.
PhylomeDBiQ1ZXI8.

Enzyme and pathway databases

ReactomeiR-DDI-975578. Reactions specific to the complex N-glycan synthesis pathway.

Miscellaneous databases

PROiQ1ZXI8.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 2 hits.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
IPR013780. Glyco_hydro_b.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 2 hits.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 2 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMANE_DICDI
AccessioniPrimary (citable) accession number: Q1ZXI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 2, 2006
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.