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Q1ZXI8

- MANE_DICDI

UniProt

Q1ZXI8 - MANE_DICDI

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Protein

Alpha-mannosidase E

Gene

manE

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi67 – 671ZincBy similarity
Metal bindingi69 – 691ZincBy similarity
Active sitei150 – 1501NucleophileBy similarity
Metal bindingi150 – 1501ZincBy similarity
Metal bindingi409 – 4091ZincBy similarity

GO - Molecular functioni

  1. alpha-mannosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase E (EC:3.2.1.24)
Gene namesi
Name:manE
ORF Names:DDB_G0278651
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278651. manE.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 10721051ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1073 – 109321HelicalSequence AnalysisAdd
BLAST
Topological domaini1094 – 112330CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 11231102Alpha-mannosidase EPRO_0000327844Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi2 – 21N-linked (GlcNAc...)Sequence Analysis
Glycosylationi38 – 381N-linked (GlcNAc...)Sequence Analysis
Glycosylationi140 – 1401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi521 – 5211N-linked (GlcNAc...)Sequence Analysis
Glycosylationi675 – 6751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi858 – 8581N-linked (GlcNAc...)Sequence Analysis
Glycosylationi887 – 8871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi975 – 9751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi990 – 9901N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231615.

Structurei

3D structure databases

ProteinModelPortaliQ1ZXI8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi451 – 4566Poly-Asn
Compositional biasi1020 – 10234Poly-Asn

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0383.
InParanoidiQ1ZXI8.
OMAiTRESSIL.
PhylomeDBiQ1ZXI8.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 2 hits.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 2 hits.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 2 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q1ZXI8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKTKLIKII FVIGVWILLS TFIINIYNEN FEIVNYNNNS QSLKKIFQLN
60 70 80 90 100
GDLKDEKLSI FLIPHSHCDS GWLQDYDWYY NHVVQYILSG IVNELNLDKE
110 120 130 140 150
KKFNWVEIGG WVQNDEATAT IDDVITQMTQ GHQWLKDTLN YTIEYAWQID
160 170 180 190 200
PFGYSSSTPT IFSSMGIKGL IINRVSDDVK SYMKSAKEME FIWKGSESLG
210 220 230 240 250
EQSQMLVSTL NVHYDYPKHI DPKKDFSLEE RVKGFTKYIK DLSNTRESSI
260 270 280 290 300
LMIPLGDDFR YSNAKNEFSV SKEWLKVIQD NKEKYNIKEI KYATIDEYFI
310 320 330 340 350
ALEDEFMNKL GKTVEENLKT GFSSTLSLYN KDFFPYSTGD LEYWTGYYTT
360 370 380 390 400
RPLLKRLIRE SSLLQKSSDI LYTLAIGENS NNQIDINNLQ TLSNQLNENR
410 420 430 440 450
NTIALVQHHD IVTGTSRSFV LNDNFQRLQK SRISNYNIIS NSLEYLLNKD
460 470 480 490 500
NNNNENTDST NEPFNFENVI DLSNEINNQY SLVFHNTLGW EVNQHVSFRI
510 520 530 540 550
KVNKNDNQLL ESIQLVEAIS NKTIQIQIIP IQDDSNCQSI ENNYIVFAII
560 570 580 590 600
NLPPLGLNTY YLSISSNEDS KPNTILSKPK LLKKGNEINF NNNRFKVEFE
610 620 630 640 650
SNGLISKITD KNSNEIKTIE QTFHQYSTKK SGPYIFNVKG GKKHGFLENP
660 670 680 690 700
DKFIYHDGPL VSQLTMLYGV DDGCNVTSIV VQRIYKNNEI DNSNSKSLIT
710 720 730 740 750
ENYIETGYSI NGDMNRETTI NYKVKDLEND DIFYTDNGLE SRKRIYNHDR
760 770 780 790 800
TVNQNYYPVL GYIKLKETSE NNNHQYTVYV DRSVGATSPS DGEMEIMIHR
810 820 830 840 850
TMDTDDWKGV NWPSKDIGRS DAKLYFNMDL VNNQLQNEKR ISLHITNQPI
860 870 880 890 900
MFVKKHNNQT GYLLKYSPLS NQLPSNIHLQ SLLTLKNNTV GLRLFNIHEV
910 920 930 940 950
DSNTQSTTDT DKSTLFNELE ISNFIETGLS FLKLTKNNLI DKYSTRINKK
960 970 980 990 1000
FPVKCGESEY SFINEKPSSI IFSNNGTNNI IDGENKGENN KTIETIQIKP
1010 1020 1030 1040 1050
HEIKSFTFNF NFQLDQIIPN NNNYAINKEL NNEYIEQSIE NQRYEYDFSF
1060 1070 1080 1090 1100
FPIKPTFYDD RGKYNRPNHL ALILSLSIGT PAGILIIVIA LVVIYKKRKN
1110 1120
RKTLTSSYSL LNLILKDRAD SSP
Length:1,123
Mass (Da):129,935
Last modified:May 2, 2006 - v1
Checksum:i7EAB06BD0CC3966C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000023 Genomic DNA. Translation: EAS66899.1.
RefSeqiXP_001134583.1. XM_001134583.1.

Genome annotation databases

EnsemblProtistsiDDB0231615; DDB0231615; DDB_G0278651.
GeneIDi8621484.
KEGGiddi:DDB_G0278651.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000023 Genomic DNA. Translation: EAS66899.1 .
RefSeqi XP_001134583.1. XM_001134583.1.

3D structure databases

ProteinModelPortali Q1ZXI8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231615.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231615 ; DDB0231615 ; DDB_G0278651 .
GeneIDi 8621484.
KEGGi ddi:DDB_G0278651.

Organism-specific databases

dictyBasei DDB_G0278651. manE.

Phylogenomic databases

eggNOGi COG0383.
InParanoidi Q1ZXI8.
OMAi TRESSIL.
PhylomeDBi Q1ZXI8.

Family and domain databases

Gene3Di 1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 2 hits.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view ]
Pfami PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 2 hits.
[Graphical view ]
SMARTi SM00872. Alpha-mann_mid. 1 hit.
[Graphical view ]
SUPFAMi SSF74650. SSF74650. 2 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiMANE_DICDI
AccessioniPrimary (citable) accession number: Q1ZXI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 2, 2006
Last modified: November 26, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3