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Protein

Alpha-mannosidase E

Gene

manE

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi67ZincBy similarity1
Metal bindingi69ZincBy similarity1
Active sitei150NucleophileBy similarity1
Metal bindingi150ZincBy similarity1
Metal bindingi409ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DDI-975578 Reactions specific to the complex N-glycan synthesis pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase E (EC:3.2.1.24)
Gene namesi
Name:manE
ORF Names:DDB_G0278651
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278651 manE

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 1072ExtracellularSequence analysisAdd BLAST1051
Transmembranei1073 – 1093HelicalSequence analysisAdd BLAST21
Topological domaini1094 – 1123CytoplasmicSequence analysisAdd BLAST30

GO - Cellular componenti

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000032784422 – 1123Alpha-mannosidase EAdd BLAST1102

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi2N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi38N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi140N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi521N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi675N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi858N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi887N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi975N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi990N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ1ZXI8

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0231615

Structurei

3D structure databases

ProteinModelPortaliQ1ZXI8
SMRiQ1ZXI8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi451 – 456Poly-Asn6
Compositional biasi1020 – 1023Poly-Asn4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1958 Eukaryota
ENOG410XQMZ LUCA
InParanoidiQ1ZXI8
OMAiLMVLHRQ
PhylomeDBiQ1ZXI8

Family and domain databases

Gene3Di1.20.1270.50, 1 hit
2.60.40.1180, 1 hit
3.20.110.10, 2 hits
InterProiView protein in InterPro
IPR011013 Gal_mutarotase_sf_dom
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR015341 Glyco_hydro_38_cen
IPR037094 Glyco_hydro_38_cen_sf
IPR000602 Glyco_hydro_38_N
IPR027291 Glyco_hydro_38_N_sf
IPR028995 Glyco_hydro_57/38_cen_sf
IPR013780 Glyco_hydro_b
PfamiView protein in Pfam
PF09261 Alpha-mann_mid, 1 hit
PF01074 Glyco_hydro_38, 2 hits
SMARTiView protein in SMART
SM00872 Alpha-mann_mid, 1 hit
SUPFAMiSSF74650 SSF74650, 2 hits
SSF88688 SSF88688, 1 hit
SSF88713 SSF88713, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q1ZXI8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKTKLIKII FVIGVWILLS TFIINIYNEN FEIVNYNNNS QSLKKIFQLN
60 70 80 90 100
GDLKDEKLSI FLIPHSHCDS GWLQDYDWYY NHVVQYILSG IVNELNLDKE
110 120 130 140 150
KKFNWVEIGG WVQNDEATAT IDDVITQMTQ GHQWLKDTLN YTIEYAWQID
160 170 180 190 200
PFGYSSSTPT IFSSMGIKGL IINRVSDDVK SYMKSAKEME FIWKGSESLG
210 220 230 240 250
EQSQMLVSTL NVHYDYPKHI DPKKDFSLEE RVKGFTKYIK DLSNTRESSI
260 270 280 290 300
LMIPLGDDFR YSNAKNEFSV SKEWLKVIQD NKEKYNIKEI KYATIDEYFI
310 320 330 340 350
ALEDEFMNKL GKTVEENLKT GFSSTLSLYN KDFFPYSTGD LEYWTGYYTT
360 370 380 390 400
RPLLKRLIRE SSLLQKSSDI LYTLAIGENS NNQIDINNLQ TLSNQLNENR
410 420 430 440 450
NTIALVQHHD IVTGTSRSFV LNDNFQRLQK SRISNYNIIS NSLEYLLNKD
460 470 480 490 500
NNNNENTDST NEPFNFENVI DLSNEINNQY SLVFHNTLGW EVNQHVSFRI
510 520 530 540 550
KVNKNDNQLL ESIQLVEAIS NKTIQIQIIP IQDDSNCQSI ENNYIVFAII
560 570 580 590 600
NLPPLGLNTY YLSISSNEDS KPNTILSKPK LLKKGNEINF NNNRFKVEFE
610 620 630 640 650
SNGLISKITD KNSNEIKTIE QTFHQYSTKK SGPYIFNVKG GKKHGFLENP
660 670 680 690 700
DKFIYHDGPL VSQLTMLYGV DDGCNVTSIV VQRIYKNNEI DNSNSKSLIT
710 720 730 740 750
ENYIETGYSI NGDMNRETTI NYKVKDLEND DIFYTDNGLE SRKRIYNHDR
760 770 780 790 800
TVNQNYYPVL GYIKLKETSE NNNHQYTVYV DRSVGATSPS DGEMEIMIHR
810 820 830 840 850
TMDTDDWKGV NWPSKDIGRS DAKLYFNMDL VNNQLQNEKR ISLHITNQPI
860 870 880 890 900
MFVKKHNNQT GYLLKYSPLS NQLPSNIHLQ SLLTLKNNTV GLRLFNIHEV
910 920 930 940 950
DSNTQSTTDT DKSTLFNELE ISNFIETGLS FLKLTKNNLI DKYSTRINKK
960 970 980 990 1000
FPVKCGESEY SFINEKPSSI IFSNNGTNNI IDGENKGENN KTIETIQIKP
1010 1020 1030 1040 1050
HEIKSFTFNF NFQLDQIIPN NNNYAINKEL NNEYIEQSIE NQRYEYDFSF
1060 1070 1080 1090 1100
FPIKPTFYDD RGKYNRPNHL ALILSLSIGT PAGILIIVIA LVVIYKKRKN
1110 1120
RKTLTSSYSL LNLILKDRAD SSP
Length:1,123
Mass (Da):129,935
Last modified:May 2, 2006 - v1
Checksum:i7EAB06BD0CC3966C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000023 Genomic DNA Translation: EAS66899.1
RefSeqiXP_001134583.1, XM_001134583.1

Genome annotation databases

EnsemblProtistsiEAS66899; EAS66899; DDB_G0278651
GeneIDi8621484
KEGGiddi:DDB_G0278651

Similar proteinsi

Entry informationi

Entry nameiMANE_DICDI
AccessioniPrimary (citable) accession number: Q1ZXI8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 2, 2006
Last modified: May 23, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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