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Q1ZXI8

- MANE_DICDI

UniProt

Q1ZXI8 - MANE_DICDI

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Protein
Alpha-mannosidase E
Gene
manE, DDB_G0278651
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi67 – 671Zinc By similarity
Metal bindingi69 – 691Zinc By similarity
Active sitei150 – 1501Nucleophile By similarity
Metal bindingi150 – 1501Zinc By similarity
Metal bindingi409 – 4091Zinc By similarity

GO - Molecular functioni

  1. alpha-mannosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. zinc ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_221646. Reactions specific to the complex N-glycan synthesis pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase E (EC:3.2.1.24)
Gene namesi
Name:manE
ORF Names:DDB_G0278651
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278651. manE.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 10721051Extracellular Reviewed prediction
Add
BLAST
Transmembranei1073 – 109321Helical; Reviewed prediction
Add
BLAST
Topological domaini1094 – 112330Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed prediction
Add
BLAST
Chaini22 – 11231102Alpha-mannosidase E
PRO_0000327844Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi2 – 21N-linked (GlcNAc...) Reviewed prediction
Glycosylationi38 – 381N-linked (GlcNAc...) Reviewed prediction
Glycosylationi140 – 1401N-linked (GlcNAc...) Reviewed prediction
Glycosylationi521 – 5211N-linked (GlcNAc...) Reviewed prediction
Glycosylationi675 – 6751N-linked (GlcNAc...) Reviewed prediction
Glycosylationi858 – 8581N-linked (GlcNAc...) Reviewed prediction
Glycosylationi887 – 8871N-linked (GlcNAc...) Reviewed prediction
Glycosylationi975 – 9751N-linked (GlcNAc...) Reviewed prediction
Glycosylationi990 – 9901N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231615.

Structurei

3D structure databases

ProteinModelPortaliQ1ZXI8.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi451 – 4566Poly-Asn
Compositional biasi1020 – 10234Poly-Asn

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0383.
OMAiTRESSIL.
PhylomeDBiQ1ZXI8.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 2 hits.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 2 hits.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 2 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q1ZXI8-1 [UniParc]FASTAAdd to Basket

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MNKTKLIKII FVIGVWILLS TFIINIYNEN FEIVNYNNNS QSLKKIFQLN     50
GDLKDEKLSI FLIPHSHCDS GWLQDYDWYY NHVVQYILSG IVNELNLDKE 100
KKFNWVEIGG WVQNDEATAT IDDVITQMTQ GHQWLKDTLN YTIEYAWQID 150
PFGYSSSTPT IFSSMGIKGL IINRVSDDVK SYMKSAKEME FIWKGSESLG 200
EQSQMLVSTL NVHYDYPKHI DPKKDFSLEE RVKGFTKYIK DLSNTRESSI 250
LMIPLGDDFR YSNAKNEFSV SKEWLKVIQD NKEKYNIKEI KYATIDEYFI 300
ALEDEFMNKL GKTVEENLKT GFSSTLSLYN KDFFPYSTGD LEYWTGYYTT 350
RPLLKRLIRE SSLLQKSSDI LYTLAIGENS NNQIDINNLQ TLSNQLNENR 400
NTIALVQHHD IVTGTSRSFV LNDNFQRLQK SRISNYNIIS NSLEYLLNKD 450
NNNNENTDST NEPFNFENVI DLSNEINNQY SLVFHNTLGW EVNQHVSFRI 500
KVNKNDNQLL ESIQLVEAIS NKTIQIQIIP IQDDSNCQSI ENNYIVFAII 550
NLPPLGLNTY YLSISSNEDS KPNTILSKPK LLKKGNEINF NNNRFKVEFE 600
SNGLISKITD KNSNEIKTIE QTFHQYSTKK SGPYIFNVKG GKKHGFLENP 650
DKFIYHDGPL VSQLTMLYGV DDGCNVTSIV VQRIYKNNEI DNSNSKSLIT 700
ENYIETGYSI NGDMNRETTI NYKVKDLEND DIFYTDNGLE SRKRIYNHDR 750
TVNQNYYPVL GYIKLKETSE NNNHQYTVYV DRSVGATSPS DGEMEIMIHR 800
TMDTDDWKGV NWPSKDIGRS DAKLYFNMDL VNNQLQNEKR ISLHITNQPI 850
MFVKKHNNQT GYLLKYSPLS NQLPSNIHLQ SLLTLKNNTV GLRLFNIHEV 900
DSNTQSTTDT DKSTLFNELE ISNFIETGLS FLKLTKNNLI DKYSTRINKK 950
FPVKCGESEY SFINEKPSSI IFSNNGTNNI IDGENKGENN KTIETIQIKP 1000
HEIKSFTFNF NFQLDQIIPN NNNYAINKEL NNEYIEQSIE NQRYEYDFSF 1050
FPIKPTFYDD RGKYNRPNHL ALILSLSIGT PAGILIIVIA LVVIYKKRKN 1100
RKTLTSSYSL LNLILKDRAD SSP 1123
Length:1,123
Mass (Da):129,935
Last modified:May 2, 2006 - v1
Checksum:i7EAB06BD0CC3966C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000023 Genomic DNA. Translation: EAS66899.1.
RefSeqiXP_001134583.1. XM_001134583.1.

Genome annotation databases

EnsemblProtistsiDDB0231615; DDB0231615; DDB_G0278651.
GeneIDi8621484.
KEGGiddi:DDB_G0278651.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000023 Genomic DNA. Translation: EAS66899.1 .
RefSeqi XP_001134583.1. XM_001134583.1.

3D structure databases

ProteinModelPortali Q1ZXI8.
ModBasei Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231615.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231615 ; DDB0231615 ; DDB_G0278651 .
GeneIDi 8621484.
KEGGi ddi:DDB_G0278651.

Organism-specific databases

dictyBasei DDB_G0278651. manE.

Phylogenomic databases

eggNOGi COG0383.
OMAi TRESSIL.
PhylomeDBi Q1ZXI8.

Enzyme and pathway databases

Reactomei REACT_221646. Reactions specific to the complex N-glycan synthesis pathway.

Family and domain databases

Gene3Di 1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 2 hits.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view ]
Pfami PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 2 hits.
[Graphical view ]
SMARTi SM00872. Alpha-mann_mid. 1 hit.
[Graphical view ]
SUPFAMi SSF74650. SSF74650. 2 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiMANE_DICDI
AccessioniPrimary (citable) accession number: Q1ZXI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 2, 2006
Last modified: September 3, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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