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Q1ZXI8

- MANE_DICDI

UniProt

Q1ZXI8 - MANE_DICDI

Protein

Alpha-mannosidase E

Gene

manE

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (02 May 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi67 – 671ZincBy similarity
    Metal bindingi69 – 691ZincBy similarity
    Active sitei150 – 1501NucleophileBy similarity
    Metal bindingi150 – 1501ZincBy similarity
    Metal bindingi409 – 4091ZincBy similarity

    GO - Molecular functioni

    1. alpha-mannosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_221646. Reactions specific to the complex N-glycan synthesis pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-mannosidase E (EC:3.2.1.24)
    Gene namesi
    Name:manE
    ORF Names:DDB_G0278651
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0278651. manE.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 11231102Alpha-mannosidase EPRO_0000327844Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi2 – 21N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi38 – 381N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi140 – 1401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi521 – 5211N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi675 – 6751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi858 – 8581N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi887 – 8871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi975 – 9751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi990 – 9901N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0231615.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1ZXI8.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini22 – 10721051ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1094 – 112330CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1073 – 109321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi451 – 4566Poly-Asn
    Compositional biasi1020 – 10234Poly-Asn

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 38 family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0383.
    OMAiTRESSIL.
    PhylomeDBiQ1ZXI8.

    Family and domain databases

    Gene3Di1.20.1270.50. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.110.10. 2 hits.
    InterProiIPR011013. Gal_mutarotase_SF_dom.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR013780. Glyco_hydro_13_b.
    IPR027291. Glyco_hydro_38/57_N.
    IPR015341. Glyco_hydro_38_cen.
    IPR000602. Glyco_hydro_38_N.
    IPR028995. Glyco_hydro_57/38_cen.
    [Graphical view]
    PfamiPF09261. Alpha-mann_mid. 1 hit.
    PF01074. Glyco_hydro_38. 2 hits.
    [Graphical view]
    SMARTiSM00872. Alpha-mann_mid. 1 hit.
    [Graphical view]
    SUPFAMiSSF74650. SSF74650. 2 hits.
    SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q1ZXI8-1 [UniParc]FASTAAdd to Basket

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    MNKTKLIKII FVIGVWILLS TFIINIYNEN FEIVNYNNNS QSLKKIFQLN     50
    GDLKDEKLSI FLIPHSHCDS GWLQDYDWYY NHVVQYILSG IVNELNLDKE 100
    KKFNWVEIGG WVQNDEATAT IDDVITQMTQ GHQWLKDTLN YTIEYAWQID 150
    PFGYSSSTPT IFSSMGIKGL IINRVSDDVK SYMKSAKEME FIWKGSESLG 200
    EQSQMLVSTL NVHYDYPKHI DPKKDFSLEE RVKGFTKYIK DLSNTRESSI 250
    LMIPLGDDFR YSNAKNEFSV SKEWLKVIQD NKEKYNIKEI KYATIDEYFI 300
    ALEDEFMNKL GKTVEENLKT GFSSTLSLYN KDFFPYSTGD LEYWTGYYTT 350
    RPLLKRLIRE SSLLQKSSDI LYTLAIGENS NNQIDINNLQ TLSNQLNENR 400
    NTIALVQHHD IVTGTSRSFV LNDNFQRLQK SRISNYNIIS NSLEYLLNKD 450
    NNNNENTDST NEPFNFENVI DLSNEINNQY SLVFHNTLGW EVNQHVSFRI 500
    KVNKNDNQLL ESIQLVEAIS NKTIQIQIIP IQDDSNCQSI ENNYIVFAII 550
    NLPPLGLNTY YLSISSNEDS KPNTILSKPK LLKKGNEINF NNNRFKVEFE 600
    SNGLISKITD KNSNEIKTIE QTFHQYSTKK SGPYIFNVKG GKKHGFLENP 650
    DKFIYHDGPL VSQLTMLYGV DDGCNVTSIV VQRIYKNNEI DNSNSKSLIT 700
    ENYIETGYSI NGDMNRETTI NYKVKDLEND DIFYTDNGLE SRKRIYNHDR 750
    TVNQNYYPVL GYIKLKETSE NNNHQYTVYV DRSVGATSPS DGEMEIMIHR 800
    TMDTDDWKGV NWPSKDIGRS DAKLYFNMDL VNNQLQNEKR ISLHITNQPI 850
    MFVKKHNNQT GYLLKYSPLS NQLPSNIHLQ SLLTLKNNTV GLRLFNIHEV 900
    DSNTQSTTDT DKSTLFNELE ISNFIETGLS FLKLTKNNLI DKYSTRINKK 950
    FPVKCGESEY SFINEKPSSI IFSNNGTNNI IDGENKGENN KTIETIQIKP 1000
    HEIKSFTFNF NFQLDQIIPN NNNYAINKEL NNEYIEQSIE NQRYEYDFSF 1050
    FPIKPTFYDD RGKYNRPNHL ALILSLSIGT PAGILIIVIA LVVIYKKRKN 1100
    RKTLTSSYSL LNLILKDRAD SSP 1123
    Length:1,123
    Mass (Da):129,935
    Last modified:May 2, 2006 - v1
    Checksum:i7EAB06BD0CC3966C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000023 Genomic DNA. Translation: EAS66899.1.
    RefSeqiXP_001134583.1. XM_001134583.1.

    Genome annotation databases

    EnsemblProtistsiDDB0231615; DDB0231615; DDB_G0278651.
    GeneIDi8621484.
    KEGGiddi:DDB_G0278651.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000023 Genomic DNA. Translation: EAS66899.1 .
    RefSeqi XP_001134583.1. XM_001134583.1.

    3D structure databases

    ProteinModelPortali Q1ZXI8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0231615.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0231615 ; DDB0231615 ; DDB_G0278651 .
    GeneIDi 8621484.
    KEGGi ddi:DDB_G0278651.

    Organism-specific databases

    dictyBasei DDB_G0278651. manE.

    Phylogenomic databases

    eggNOGi COG0383.
    OMAi TRESSIL.
    PhylomeDBi Q1ZXI8.

    Enzyme and pathway databases

    Reactomei REACT_221646. Reactions specific to the complex N-glycan synthesis pathway.

    Family and domain databases

    Gene3Di 1.20.1270.50. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.110.10. 2 hits.
    InterProi IPR011013. Gal_mutarotase_SF_dom.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR013780. Glyco_hydro_13_b.
    IPR027291. Glyco_hydro_38/57_N.
    IPR015341. Glyco_hydro_38_cen.
    IPR000602. Glyco_hydro_38_N.
    IPR028995. Glyco_hydro_57/38_cen.
    [Graphical view ]
    Pfami PF09261. Alpha-mann_mid. 1 hit.
    PF01074. Glyco_hydro_38. 2 hits.
    [Graphical view ]
    SMARTi SM00872. Alpha-mann_mid. 1 hit.
    [Graphical view ]
    SUPFAMi SSF74650. SSF74650. 2 hits.
    SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiMANE_DICDI
    AccessioniPrimary (citable) accession number: Q1ZXI8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 2, 2006
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3