ID   PTPS_DICDI              Reviewed;         135 AA.
AC   Q1ZXI0;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2006, sequence version 1.
DT   16-JUN-2009, entry version 21.
DE   RecName: Full=6-pyruvoyl tetrahydrobiopterin synthase;
DE            Short=PTP synthase;
DE            Short=PTPS;
DE            EC=4.2.3.12;
GN   Name=ptsA; ORFNames=DDB_G0279095;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA   Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA   Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA   Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA   Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA   Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA   Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA   Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA   Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA   Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA   Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA   Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA   Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA   Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA   Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA   Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA   Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Involved in the biosynthesis of tetrahydrobiopterin, an
CC       essential cofactor of aromatic amino acid hydroxylases. Catalyzes
CC       the transformation of 7,8-dihydroneopterin triphosphate into 6-
CC       pyruvoyl tetrahydropterin (By similarity).
CC   -!- CATALYTIC ACTIVITY: 7,8-dihydroneopterin 3'-triphosphate = 6-
CC       pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- PATHWAY: Cofactor biosynthesis; tetrahydrobiopterin biosynthesis;
CC       tetrahydrobiopterin from 2-amino-4-hydroxy-6-(erythro-1,2,3-
CC       trihydroxypropyl)-dihydropteridine triphosphate: step 1/3.
CC   -!- SUBUNIT: Homohexamer formed of two homotrimers in a head to head
CC       fashion (By similarity).
CC   -!- MISCELLANEOUS: The active site is at the interface between 2
CC       subunits. The proton acceptor Cys is on one subunit, and the
CC       charge relay system is on the other subunit.
CC   -!- SIMILARITY: Belongs to the PTPS family.
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DR   EMBL; AAFI02000027; EAS66886.1; -; Genomic_DNA.
DR   RefSeq; XP_001134570.1; -.
DR   GeneID; 4238116; -.
DR   KEGG; ddi:DDB_0232952; -.
DR   dictyBase; DDB_G0279095; ptsA.
DR   BRENDA; 4.2.3.12; 424.
DR   GO; GO:0003874; F:6-pyruvoyltetrahydropterin synthase activity; IEA:EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006729; P:tetrahydrobiopterin biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR007115; 6-PTP_synth-rel.
DR   PANTHER; PTHR12589; 6_PTP_synth; 1.
DR   Pfam; PF01242; PTPS; 1.
DR   ProDom; PD004049; PTPS_hypoth; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Lyase; Metal-binding;
KW   Tetrahydrobiopterin biosynthesis; Zinc.
FT   CHAIN         1    135       6-pyruvoyl tetrahydrobiopterin synthase.
FT                                /FTId=PRO_0000327514.
FT   ACT_SITE     36     36       Proton acceptor (By similarity).
FT   ACT_SITE     81     81       Charge relay system (By similarity).
FT   ACT_SITE    124    124       Charge relay system (By similarity).
FT   METAL        17     17       Zinc (By similarity).
FT   METAL        40     40       Zinc (By similarity).
FT   METAL        42     42       Zinc (By similarity).
SQ   SEQUENCE   135 AA;  15771 MW;  75B75E9AE96F525D CRC64;
     MSRTVILTRR EVFSSSHRLY SDKLSLEENK KIYGKCINSH GHNYVLEVSI KGAVKEDIGM
     FMNITELKEI LKEKVMDKLD HKNLENDVPE LKGIVTTTEN LSIFIWDQLF PSLKDFLYEV
     KILETENNFV VYRGE
//