Q1ZXI0 (PTPS_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-pyruvoyl tetrahydrobiopterin synthase
Short name=PTP synthase
Short name=PTPS
EC=4.2.3.12

Gene names

Name:	ptsA
ORF Names:	DDB_G0279095

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	135 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin By similarity.
Catalytic activity	7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Cofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate: step 1/3.
Subunit structure	Homohexamer formed of two homotrimers in a head to head fashion By similarity.
Miscellaneous	The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit.
Sequence similarities	Belongs to the PTPS family.

Ontologies

Keywords
Biological process	Tetrahydrobiopterin biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	folic acid biosynthetic process Inferred from direct assay. Source: dictyBase tetrahydrobiopterin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	soluble fraction Inferred from direct assay. Source: dictyBase
Molecular function	6-pyruvoyltetrahydropterin synthase activity Inferred from direct assay. Source: dictyBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 135	135	6-pyruvoyl tetrahydrobiopterin synthase		PRO_0000327514
Sites
	Active site	36	1	Proton acceptor By similarity
	Active site	81	1	Charge relay system By similarity
	Active site	124	1	Charge relay system By similarity
	Metal binding	17	1	Zinc By similarity
	Metal binding	40	1	Zinc By similarity
	Metal binding	42	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1ZXI0 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: 75B75E9AE96F525D	FASTA	135	15,771
	10 20 30 40 50 60 MSRTVILTRR EVFSSSHRLY SDKLSLEENK KIYGKCINSH GHNYVLEVSI KGAVKEDIGM 70 80 90 100 110 120 FMNITELKEI LKEKVMDKLD HKNLENDVPE LKGIVTTTEN LSIFIWDQLF PSLKDFLYEV 130 KILETENNFV VYRGE

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000027 Genomic DNA. Translation: EAS66886.1.
RefSeq	XP_001134570.1. XM_001134570.1.
3D structure databases
ProteinModelPortal	Q1ZXI0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1ZXI0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0232952; DDB0232952; DDB_G0279095.
GeneID	8621869.
GenomeReviews	Gene locus ptsA in contig CM000152_GR.
KEGG	ddi:DDB_G0279095.
Organism-specific databases
dictyBase	DDB_G0279095. ptsA.
Phylogenomic databases
eggNOG	KOG4105.
HOGENOM	HBG727155.
OMA	HKNLDKD.
PhylomeDB	Q1ZXI0.
ProtClustDB	CLSZ2445181.
Family and domain databases
InterPro	IPR007115. 6-PTP_synth-rel. [Graphical view]
PANTHER	PTHR12589. 6_PTP_synth. 1 hit.
Pfam	PF01242. PTPS. 1 hit. [Graphical view]
PIRSF	PIRSF006113. PTP_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PTPS_DICDI

Accession

Primary (citable) accession number: Q1ZXI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 2, 2006
Last modified:	September 21, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)