ID   CP51_DICDI              Reviewed;         466 AA.
AC   Q1ZXH9;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2006, sequence version 1.
DT   16-JUN-2009, entry version 34.
DE   RecName: Full=Probable lanosterol 14-alpha demethylase;
DE            Short=LDM;
DE            EC=1.14.13.70;
DE   AltName: Full=Cytochrome P450 51;
DE   AltName: Full=Sterol 14-demethylase;
DE   AltName: Full=P450-14DM;
GN   Name=cyp51; ORFNames=DDB_G0279403;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA   Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA   Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA   Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA   Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA   Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA   Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA   Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA   Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA   Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA   Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA   Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA   Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA   Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA   Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA   Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA   Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Catalyzes C14-demethylation of lanosterol which is
CC       critical for ergosterol biosynthesis. It transforms lanosterol
CC       into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: Obtusifoliol + 3 O(2) + 3 NADPH = 4-alpha-
CC       methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3
CC       NADP(+) + 4 H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- PATHWAY: Steroid biosynthesis; zymosterol biosynthesis; zymosterol
CC       from lanosterol: step 1/6.
CC   -!- SUBCELLULAR LOCATION: Membrane (Potential). Membrane; Single-pass
CC       membrane protein.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; AAFI02000030; EAS66884.1; -; Genomic_DNA.
DR   RefSeq; XP_001134568.1; -.
DR   GeneID; 4238117; -.
DR   KEGG; ddi:DDB_0232962; -.
DR   dictyBase; DDB_G0279403; cyp51.
DR   BRENDA; 1.14.13.70; 424.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0008398; F:sterol 14-demethylase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017973; Cyt_P450_C.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002403; Cyt_P450_E_grp-IV.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00465; EP450IV.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Heme; Iron; Lipid synthesis; Membrane;
KW   Metal-binding; Monooxygenase; NADP; Oxidoreductase;
KW   Steroid biosynthesis; Sterol biosynthesis.
FT   CHAIN         1    466       Probable lanosterol 14-alpha demethylase.
FT                                /FTId=PRO_0000318989.
FT   METAL       413    413       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   466 AA;  52686 MW;  96E98F64C579EA90 CRC64;
     MIGTIAVLVI AILVIFAFKK SPSNIPPIVE TIPFIGCFYQ FAKNPLQLVR NSYDRLGEIF
     TLHLMGFKMT FVLGPEAQAL FFRGTDEELS PKEAYRFVTP VFGKGVVYDS ETEIMYEQLR
     FVKNGLVLSQ LKKAVGIIQE ETEKYFETKW GDSGEIDLLY EMNKLTILTA SRCLMGKSIN
     KSLGQSGQLA DLYHELEEGL NPISFFFPNL PLPSFKKRDA ARAKVAAIFH SIIQERRRST
     DDSVDDVLYT LMNSKYKDGS VLEDEQIVGL MIGLLFAGQH TSSITLTYTI FYLLNNLEYF
     DETQKDINDI VQKENQGEIN FDGLKRMNRL ETVIREVLRL HPPLIFLMRK VMTPMEYKGK
     TIPAGHILAV SPQVGMRLPT VYKNPDSFEP KRFDVEDKTP FSFIAFGGGK HGCPGENFGI
     LQIKTIWTVL STKYNLEVGP VPPTDFTSLV AGPKGPCMVK YSKKQK
//