Reviewed, UniProtKB/Swiss-Prot Q1ZXH9 (CP51_DICDI)

Names and origin

Protein names

Recommended name:
    Probable lanosterol 14-alpha demethylase
      Short name=LDM
    EC=1.14.13.70
Alternative name(s):
    Cytochrome P450 51
    Sterol 14-demethylase
    P450-14DM

Gene names

Name:	cyp51
ORF Names:	DDB_G0279403

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol By similarity.
Catalytic activity	Obtusifoliol + 3 O2 + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP+ + 4 H2O.
Cofactor	Heme group By similarity.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Subcellular location	Membrane Potential. Membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sterol 14-demethylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Probable lanosterol 14-alpha demethylase		PRO_0000318989
Sites
	Metal binding	413	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1ZXH9-1 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: 96E98F64C579EA90	FASTA	466	52,686
	10 20 30 40 50 60 MIGTIAVLVI AILVIFAFKK SPSNIPPIVE TIPFIGCFYQ FAKNPLQLVR NSYDRLGEIF 70 80 90 100 110 120 TLHLMGFKMT FVLGPEAQAL FFRGTDEELS PKEAYRFVTP VFGKGVVYDS ETEIMYEQLR 130 140 150 160 170 180 FVKNGLVLSQ LKKAVGIIQE ETEKYFETKW GDSGEIDLLY EMNKLTILTA SRCLMGKSIN 190 200 210 220 230 240 KSLGQSGQLA DLYHELEEGL NPISFFFPNL PLPSFKKRDA ARAKVAAIFH SIIQERRRST 250 260 270 280 290 300 DDSVDDVLYT LMNSKYKDGS VLEDEQIVGL MIGLLFAGQH TSSITLTYTI FYLLNNLEYF 310 320 330 340 350 360 DETQKDINDI VQKENQGEIN FDGLKRMNRL ETVIREVLRL HPPLIFLMRK VMTPMEYKGK 370 380 390 400 410 420 TIPAGHILAV SPQVGMRLPT VYKNPDSFEP KRFDVEDKTP FSFIAFGGGK HGCPGENFGI 430 440 450 460 LQIKTIWTVL STKYNLEVGP VPPTDFTSLV AGPKGPCMVK YSKKQK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000030 Genomic DNA. Translation: EAS66884.1.
RefSeq	XP_001134568.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4238117.
KEGG	ddi:DDB_0232962.
Organism-specific databases
dictyBase	DDB_G0279403. cyp51.
Enzyme and pathway databases
BRENDA	1.14.13.70. 424.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP51_DICDI

Accession

Primary (citable) accession number: Q1ZXH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 2, 2006
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)