ID   GMDS_DICDI              Reviewed;         356 AA.
AC   Q1ZXF7;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2006, sequence version 1.
DT   16-JUN-2009, entry version 23.
DE   RecName: Full=GDP-mannose 4,6 dehydratase;
DE            EC=4.2.1.47;
DE   AltName: Full=GDP-D-mannose dehydratase;
DE            Short=GMD;
GN   Name=gmd; ORFNames=DDB_G0284553;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA   Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA   Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA   Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA   Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA   Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA   Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA   Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA   Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA   Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA   Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA   Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA   Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA   Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA   Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA   Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA   Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Conversion of GDP-D-mannose to GDP-4-keto-6-D-
CC       deoxymannose.
CC   -!- CATALYTIC ACTIVITY: GDP-mannose = GDP-4-dehydro-6-deoxy-D-mannose
CC       + H(2)O.
CC   -!- COFACTOR: NADP (By similarity).
CC   -!- PATHWAY: Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis
CC       via de novo pathway; GDP-L-fucose from GDP-D-mannose: step 1/2.
CC   -!- SIMILARITY: Belongs to the GDP-mannose 4,6-dehydratase family.
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DR   EMBL; AAFI02000066; EAS66860.1; -; Genomic_DNA.
DR   RefSeq; XP_001134543.1; -.
DR   GeneID; 4238151; -.
DR   KEGG; ddi:DDB_0231676; -.
DR   dictyBase; DDB_G0284553; gmd.
DR   BRENDA; 4.2.1.47; 424.
DR   GO; GO:0005622; C:intracellular; IEA:InterPro.
DR   GO; GO:0050662; F:coenzyme binding; IEA:InterPro.
DR   GO; GO:0008446; F:GDP-mannose 4,6-dehydratase activity; IEA:EC.
DR   GO; GO:0019673; P:GDP-mannose metabolic process; IEA:InterPro.
DR   InterPro; IPR001509; Epimerase_deHydtase.
DR   InterPro; IPR006368; GDP_Man_deHydtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR10366:SF32; GDP_mann_dehyd; 1.
DR   Pfam; PF01370; Epimerase; 1.
DR   TIGRFAMs; TIGR01472; gmd; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Lyase; NADP.
FT   CHAIN         1    356       GDP-mannose 4,6 dehydratase.
FT                                /FTId=PRO_0000327628.
FT   ACT_SITE    138    138       By similarity.
FT   ACT_SITE    140    140       Nucleophile (By similarity).
FT   ACT_SITE    162    162       Nucleophile (By similarity).
FT   BINDING     166    166       NADP (By similarity).
SQ   SEQUENCE   356 AA;  41061 MW;  9DB1B513B28E16CA CRC64;
     MSEERKVALI TGITGQDGSY LTEFLISKGY YVHGIIQKIF HHFNTIVKNI YIKIDMLKEK
     ESLTLHYGDL TDASNLHSIV SKVNPTEIYN LGAQSHVKVS FDMSEYTGDV DGLGCLRLLD
     AIRSCGMEKK VKYYQASTSE LYGKVQEIPQ SETTPFYPRS PYAVAKQYAY WIVVNYREAY
     DMYACNGILF NHESPRRGPT FVTRKITRFV AGIACGRDEI LYLGNINAKR DWGHARDYVE
     AMWLMLQQEK PEDFVIATGE THSVREFVEK SFKEIDIIIK WRGEAEKEEG YCEKTGKVYV
     KIDEKYYRPT EVDLLLGNPN KAKKLLQWQI KTSFGELVKE MVAKDIEYIK NGDKYN
//