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Protein

Ribonuclease E

Gene

rne

Organism
Photobacterium angustum (strain S14 / CCUG 15956) (Vibrio sp. (strain S14 / CCUG 15956))
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
  • Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per homotetramer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi302Magnesium; catalyticUniRule annotation1
Metal bindingi345Magnesium; catalyticUniRule annotation1
Metal bindingi403Zinc; shared with dimeric partnerUniRule annotation1
Metal bindingi406Zinc; shared with dimeric partnerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonucleaseUniRule annotation, Hydrolase, Nuclease, RNA-bindingUniRule annotation
Biological processrRNA processingUniRule annotation, tRNA processingUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation, ZincUniRule annotation

Enzyme and pathway databases

BRENDAi3.1.26.12. 11794.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease EUniRule annotation (EC:3.1.26.12UniRule annotation)
Short name:
RNase EUniRule annotation
Gene namesi
Name:rneUniRule annotation
ORF Names:VAS14_05283Imported
OrganismiPhotobacterium angustum (strain S14 / CCUG 15956) (Vibrio sp. (strain S14 / CCUG 15956))Imported
Taxonomic identifieri314292 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaePhotobacterium
Proteomesi
  • UP000001603 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membraneUniRule annotation, Cell membrane, CytoplasmUniRule annotation, Membrane

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, Rnase E assembles into a homotetramer formed by a dimer of dimers.UniRule annotation

Binary interactionsi

Show more details

Protein-protein interaction databases

IntActiQ1ZS71. 3 interactors.
MINTiMINT-8049276.
STRINGi314292.VAS14_05283.

Structurei

3D structure databases

ProteinModelPortaliQ1ZS71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 119S1 motifInterPro annotationAdd BLAST81

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni403 – 406Required for zinc-mediated homotetramerization and catalytic activityUniRule annotation4

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili676 – 706Sequence analysisAdd BLAST31

Sequence similaritiesi

Belongs to the RNase E/G family. RNase E subfamily.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiENOG4107QQB. Bacteria.
COG1530. LUCA.
OrthoDBiPOG091H05KQ.

Family and domain databases

HAMAPiMF_00970. RNase_E. 1 hit.
InterProiView protein in InterPro
IPR012340. NA-bd_OB-fold.
IPR019307. RNA-bd_AU-1/RNase_E/G.
IPR028878. RNase_E.
IPR004659. RNase_E/G.
IPR022967. S1_dom.
IPR003029. S1_domain.
PfamiView protein in Pfam
PF10150. RNase_E_G. 1 hit.
PF00575. S1. 1 hit.
SMARTiView protein in SMART
SM00316. S1. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00757. RNaseEG. 1 hit.
PROSITEiView protein in PROSITE
PS50126. S1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1ZS71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRMLINATQ KEELRVALVD GQKLFDLDIE SPGHESKKAN IYKGRITRIE
60 70 80 90 100
PSLEAAFVDY GAERHGFLPL KEVAKDYFPA NYSYQGRPNI KEVLKEGQEV
110 120 130 140 150
IVQVDKEERG NKGAALTTFI SLAGSYLVLM PNNPRAGGIS RRIEGEERTQ
160 170 180 190 200
LKAALSTLDL PQGMGLIVRT AGVGKSGEEL EWDLNVLLNH WNAVKEAADS
210 220 230 240 250
QSAPFLIHQE SNVIARAIRD YLRRDIGEIL IDSPKIFDRA RDHIKLVRPD
260 270 280 290 300
FLSRVKRYEG EVPLFSHYQI ESQIDSAFQR EVRLPSGGSV VIDPTEALTS
310 320 330 340 350
IDINSARATK GGDIEETALQ TNLEAADEIA RQLRLRDLGG LVVIDFIDMT
360 370 380 390 400
PVRHQREVEN RLREAVRMDR ARVQIGRISR FGLLEMSRQR LSPSLAEASH
410 420 430 440 450
HVCPRCSGTG VVRDNESLSL SILRLIEEEA LKDNSSQVLA IVPVPVASYL
460 470 480 490 500
LNEKRRSVQH IEKYHGVRVV IVPNSDMETP HFEVLRIREG EEQDTLSYHI
510 520 530 540 550
PKTLEALKEA EAIEAPQPER VIKKREEPVL QGFTAPKQPA PVAKPAAKPT
560 570 580 590 600
PKVTAKPATE STKKPGLLSR FFSALASFFS GSPEQQQEKP VEAKVEQNNN
610 620 630 640 650
QNNRRNNNRR NDRSDNRRNN RDNSRDNNRR NNRQDNNDVE NNDERQERNN
660 670 680 690 700
EQRQNRRRND RNDKNRQQQN DKAAKAPRQN RNNDEAKEQK RQSRKEEIQA
710 720 730 740 750
QKQQRQQQGE QTAKATTEQP ADAQRDSEEK AVKVKQRRQR RQLRKKVRIT
760 770 780 790 800
DNVEKSVEQQ NATVDAVEKV EAVETAVEQT PSPLMALGTS IAKEAQDKPE
810 820 830 840 850
KTQVVSDEQT DNEEQEGTQR RNRRSPRHLR ASGQRRRRMR DTRVEKSDFV
860 870 880 890 900
TNEEANAIEQ AAIDAADSIS EMVNVVSIVE KPKTMRPTSG VAIPEMAMGK
910 920 930 940 950
VWSRIKPAVE APVEKNITQE KADTADMIDE VETNDIIINT PVVEAKSEIV
960 970 980 990 1000
AVETTTLSGV AIPEMAMGKV IPRYTAEETT VEMKSEPAPE QVIEKPVIVE
1010 1020 1030 1040 1050
EVAAPVIKTE ETVEIKSPVT AVVDLPAVAK AVMATSPRGF HAASAMTKAP
1060 1070 1080 1090
APEEHVSGSV VQAAPLRERS NTRAAGSQTA TNSAAAPMTK PSFD
Length:1,094
Mass (Da):122,596
Last modified:May 2, 2006 - v1
Checksum:i6665693F00DC299E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAOJ01000002 Genomic DNA. Translation: EAS65106.1.
RefSeqiWP_005368733.1. NZ_CH902600.1.

Genome annotation databases

EnsemblBacteriaiEAS65106; EAS65106; VAS14_05283.

Similar proteinsi

Entry informationi

Entry nameiQ1ZS71_PHOAS
AccessioniPrimary (citable) accession number: Q1ZS71
Entry historyiIntegrated into UniProtKB/TrEMBL: May 2, 2006
Last sequence update: May 2, 2006
Last modified: September 27, 2017
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteome, Reference proteomeImported