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Q1XHL7

- GRK7B_DANRE

UniProt

Q1XHL7 - GRK7B_DANRE

Protein

G protein-coupled receptor kinase 7B

Gene

grk7b

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 2 (21 Sep 2011)
      Previous versions | rss
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    Functioni

    Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).1 Publication

    Catalytic activityi

    ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.
    ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei216 – 2161ATPPROSITE-ProRule annotation
    Active sitei312 – 3121Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi193 – 2019ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. G-protein coupled receptor kinase activity Source: UniProtKB-EC
    3. rhodopsin kinase activity Source: UniProtKB

    GO - Biological processi

    1. signal transduction Source: InterPro
    2. termination of G-protein coupled receptor signaling pathway Source: InterPro
    3. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Sensory transduction, Vision

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.14. 96826.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G protein-coupled receptor kinase 7B (EC:2.7.11.14, EC:2.7.11.16)
    Alternative name(s):
    G protein-coupled receptor kinase 7-2
    Gene namesi
    Name:grk7b
    Synonyms:grk7-2
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 18

    Organism-specific databases

    ZFINiZDB-GENE-030904-3. grk7b.

    Subcellular locationi

    Membrane By similarity; Lipid-anchor By similarity

    GO - Cellular componenti

    1. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 545545G protein-coupled receptor kinase 7BPRO_0000412814Add
    BLAST
    Propeptidei546 – 5483Removed in mature formBy similarityPRO_0000412815

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei33 – 331Phosphoserine; by PKA
    Modified residuei545 – 5451Cysteine methyl esterSequence Analysis
    Lipidationi545 – 5451S-geranylgeranyl cysteineSequence Analysis

    Post-translational modificationi

    Phosphorylation at Ser-33 is regulated by light and activated by cAMP.By similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Phosphoprotein, Prenylation

    Expressioni

    Tissue specificityi

    Expressed in the eyes (at protein level). Expressed in the eyes, the pineal gland and in the brain.1 Publication

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini53 – 172120RGSPROSITE-ProRule annotationAdd
    BLAST
    Domaini187 – 446260Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini447 – 51266AGC-kinase C-terminalAdd
    BLAST

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 RGS domain.PROSITE-ProRule annotation

    Phylogenomic databases

    GeneTreeiENSGT00730000110751.
    HOVERGENiHBG004532.
    InParanoidiQ1XHL7.
    OMAiGIEMERI.
    OrthoDBiEOG7V1FQK.
    TreeFamiTF313940.

    Family and domain databases

    InterProiIPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view]
    PRINTSiPR00717. GPCRKINASE.
    SMARTiSM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q1XHL7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDMGGLKNL VANTDYLKAQ SLDEKEIKKR RCSLGLPHLG NCTDVRVSVS    50
    KGFEDICEQQ PIGRACFRQF LSVSSPEYLA AAELLDELNC WNLAEAEAKE 100
    EARLNIINKF CKADSKSFLA FLTEDEAEKC KAVSEKDFEE VMMGQVKEAT 150
    QKFLRGQPFE EYQTSLFFDR FVQWKKFEKQ PITDKYFYEF RTLGKGGFGE 200
    VCGVQVKTTG QMYACKKLDK KRLKKKSGEK MALLEKKILE MVNSLFIVNL 250
    AYAFDTKTHL CLVMTLMSGG DLKYHIFHVG EVGIEMERII HYTAQITSGI 300
    LHLHSMDIVY RDMKPENVLL DCQGQCRLSD LGLAVELPNG KTTTQKAGTK 350
    GYMAPEILKQ EPYRTSVDWW ALGCSIYEMV AGRVPFRDHK EKVAKEELLR 400
    RTLEDEVKFE HRNFDAPSKD IISLFLKRNI EDRLGSNDDP RKHEFFKSIN 450
    FPRLEAGLIP PPWEPKANVV YAKDTGDIRE FSDVKGVKFD ANDEKFFKEF 500
    STGVVPIAWQ QEMIDSGLFD ELSDPNRKES AAGLEDEEQQ KSKSCTLL 548
    Length:548
    Mass (Da):62,609
    Last modified:September 21, 2011 - v2
    Checksum:i939D0B13095FECF2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti47 – 471V → A in BAE92859. (PubMed:16787417)Curated
    Sequence conflicti47 – 471V → A in AAI62212. 1 PublicationCurated
    Sequence conflicti339 – 3391N → D in BAE92859. (PubMed:16787417)Curated
    Sequence conflicti339 – 3391N → D in AAI62212. 1 PublicationCurated
    Sequence conflicti339 – 3391N → D in AAI62221. 1 PublicationCurated
    Sequence conflicti412 – 4121R → Q in BAE92859. (PubMed:16787417)Curated
    Sequence conflicti412 – 4121R → Q in AAI62212. 1 PublicationCurated
    Sequence conflicti412 – 4121R → Q in AAI62221. 1 PublicationCurated
    Sequence conflicti504 – 5041V → A in BAE92859. (PubMed:16787417)Curated
    Sequence conflicti504 – 5041V → A in AAI62212. 1 PublicationCurated
    Sequence conflicti504 – 5041V → A in AAI62221. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB212996 mRNA. Translation: BAE92859.1.
    BC162212 mRNA. Translation: AAI62212.1.
    BC162221 mRNA. Translation: AAI62221.1.
    UniGeneiDr.88577.

    Genome annotation databases

    EnsembliENSDART00000077898; ENSDARP00000072364; ENSDARG00000055534.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB212996 mRNA. Translation: BAE92859.1 .
    BC162212 mRNA. Translation: AAI62212.1 .
    BC162221 mRNA. Translation: AAI62221.1 .
    UniGenei Dr.88577.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000077898 ; ENSDARP00000072364 ; ENSDARG00000055534 .

    Organism-specific databases

    ZFINi ZDB-GENE-030904-3. grk7b.

    Phylogenomic databases

    GeneTreei ENSGT00730000110751.
    HOVERGENi HBG004532.
    InParanoidi Q1XHL7.
    OMAi GIEMERI.
    OrthoDBi EOG7V1FQK.
    TreeFami TF313940.

    Enzyme and pathway databases

    BRENDAi 2.7.11.14. 96826.

    Family and domain databases

    InterProi IPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view ]
    PRINTSi PR00717. GPCRKINASE.
    SMARTi SM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "GRK1 and GRK7: unique cellular distribution and widely different activities of opsin phosphorylation in the zebrafish rods and cones."
      Wada Y., Sugiyama J., Okano T., Fukada Y.
      J. Neurochem. 98:824-837(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, FUNCTION.
    2. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiGRK7B_DANRE
    AccessioniPrimary (citable) accession number: Q1XHL7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 21, 2011
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 63 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3