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Q1XHL7 (GRK7B_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G protein-coupled receptor kinase 7B

EC=2.7.11.14
EC=2.7.11.16
Alternative name(s):
G protein-coupled receptor kinase 7-2
Gene names
Name:grk7b
Synonyms:grk7-2
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length548 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO). Ref.1

Catalytic activity

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.

ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.

Subcellular location

Membrane; Lipid-anchor By similarity.

Tissue specificity

Expressed in the eyes (at protein level). Expressed in the eyes, the pineal gland and in the brain. Ref.1

Post-translational modification

Phosphorylation at Ser-33 is regulated by light and activated by cAMP By similarity.

Miscellaneous

Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 protein kinase domain.

Contains 1 RGS domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 545545G protein-coupled receptor kinase 7B
PRO_0000412814
Propeptide546 – 5483Removed in mature form By similarity
PRO_0000412815

Regions

Domain53 – 172120RGS
Domain187 – 446260Protein kinase
Domain447 – 51266AGC-kinase C-terminal
Nucleotide binding193 – 2019ATP By similarity

Sites

Active site3121Proton acceptor By similarity
Binding site2161ATP By similarity

Amino acid modifications

Modified residue331Phosphoserine; by PKA
Modified residue5451Cysteine methyl ester Potential
Lipidation5451S-geranylgeranyl cysteine Potential

Experimental info

Sequence conflict471V → A in BAE92859. Ref.1
Sequence conflict471V → A in AAI62212. Ref.2
Sequence conflict3391N → D in BAE92859. Ref.1
Sequence conflict3391N → D in AAI62212. Ref.2
Sequence conflict3391N → D in AAI62221. Ref.2
Sequence conflict4121R → Q in BAE92859. Ref.1
Sequence conflict4121R → Q in AAI62212. Ref.2
Sequence conflict4121R → Q in AAI62221. Ref.2
Sequence conflict5041V → A in BAE92859. Ref.1
Sequence conflict5041V → A in AAI62212. Ref.2
Sequence conflict5041V → A in AAI62221. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q1XHL7 [UniParc].

Last modified September 21, 2011. Version 2.
Checksum: 939D0B13095FECF2

FASTA54862,609
        10         20         30         40         50         60 
MSDMGGLKNL VANTDYLKAQ SLDEKEIKKR RCSLGLPHLG NCTDVRVSVS KGFEDICEQQ 

        70         80         90        100        110        120 
PIGRACFRQF LSVSSPEYLA AAELLDELNC WNLAEAEAKE EARLNIINKF CKADSKSFLA 

       130        140        150        160        170        180 
FLTEDEAEKC KAVSEKDFEE VMMGQVKEAT QKFLRGQPFE EYQTSLFFDR FVQWKKFEKQ 

       190        200        210        220        230        240 
PITDKYFYEF RTLGKGGFGE VCGVQVKTTG QMYACKKLDK KRLKKKSGEK MALLEKKILE 

       250        260        270        280        290        300 
MVNSLFIVNL AYAFDTKTHL CLVMTLMSGG DLKYHIFHVG EVGIEMERII HYTAQITSGI 

       310        320        330        340        350        360 
LHLHSMDIVY RDMKPENVLL DCQGQCRLSD LGLAVELPNG KTTTQKAGTK GYMAPEILKQ 

       370        380        390        400        410        420 
EPYRTSVDWW ALGCSIYEMV AGRVPFRDHK EKVAKEELLR RTLEDEVKFE HRNFDAPSKD 

       430        440        450        460        470        480 
IISLFLKRNI EDRLGSNDDP RKHEFFKSIN FPRLEAGLIP PPWEPKANVV YAKDTGDIRE 

       490        500        510        520        530        540 
FSDVKGVKFD ANDEKFFKEF STGVVPIAWQ QEMIDSGLFD ELSDPNRKES AAGLEDEEQQ 


KSKSCTLL 

« Hide

References

« Hide 'large scale' references
[1]"GRK1 and GRK7: unique cellular distribution and widely different activities of opsin phosphorylation in the zebrafish rods and cones."
Wada Y., Sugiyama J., Okano T., Fukada Y.
J. Neurochem. 98:824-837(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, FUNCTION.
[2]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB212996 mRNA. Translation: BAE92859.1.
BC162212 mRNA. Translation: AAI62212.1.
BC162221 mRNA. Translation: AAI62221.1.
UniGeneDr.88577.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSDART00000077898; ENSDARP00000072364; ENSDARG00000055534.

Organism-specific databases

ZFINZDB-GENE-030904-3. grk7b.

Phylogenomic databases

GeneTreeENSGT00730000110751.
HOVERGENHBG004532.
InParanoidQ1XHL7.
OMAGIEMERI.
OrthoDBEOG7V1FQK.
TreeFamTF313940.

Enzyme and pathway databases

BRENDA2.7.11.14. 96826.

Family and domain databases

InterProIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSPR00717. GPCRKINASE.
SMARTSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGRK7B_DANRE
AccessionPrimary (citable) accession number: Q1XHL7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: September 21, 2011
Last modified: February 19, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families