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Q1XH17

- TRI72_MOUSE

UniProt

Q1XH17 - TRI72_MOUSE

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Protein
Tripartite motif-containing protein 72
Gene
Trim72, Mg53
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca2+-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri14 – 5744RING-type
Add
BLAST
Zinc fingeri81 – 12242B box-type
Add
BLAST

GO - Molecular functioni

  1. phosphatidylserine binding Source: UniProtKB
  2. protein binding Source: MGI
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. exocytosis Source: UniProtKB
  2. membrane budding Source: UniProtKB
  3. muscle organ development Source: UniProtKB
  4. muscle system process Source: UniProtKB
  5. plasma membrane repair Source: UniProtKB
  6. protein homooligomerization Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Transport

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Tripartite motif-containing protein 72
Alternative name(s):
Mitsugumin-53
Short name:
Mg53
Gene namesi
Name:Trim72
Synonyms:Mg53
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:3612190. Trim72.

Subcellular locationi

Cell membranesarcolemma. Cytoplasmic vesicle membrane
Note: Tethered to plasma membrane and cytoplasmic vesicles via its interaction with phosphatidylserine.2 Publications

GO - Cellular componenti

  1. cytoplasmic vesicle membrane Source: UniProtKB
  2. sarcolemma Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Disruption phenotypei

Viable until at least 11 months of age under unstressed conditions, and develop progressive muscle pathology with age. Mice show progressive myopathy and reduced exercise capability, associated with defective membrane-repair capacity.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi14 – 141C → A: Does not affect homooligomerization. 1 Publication
Mutagenesisi17 – 171C → A: Does not affect homooligomerization. 1 Publication
Mutagenesisi29 – 291C → A: Does not affect homooligomerization. 1 Publication
Mutagenesisi34 – 341C → A: Does not affect homooligomerization. 1 Publication
Mutagenesisi37 – 371C → A: Does not affect homooligomerization. 1 Publication
Mutagenesisi53 – 531C → A: Does not affect homooligomerization. 1 Publication
Mutagenesisi55 – 551C → A: Does not affect homooligomerization. 1 Publication
Mutagenesisi56 – 561C → A: Does not affect homooligomerization. 1 Publication
Mutagenesisi86 – 861C → A: Does not affect homooligomerization. 1 Publication
Mutagenesisi97 – 971C → A: Does not affect homooligomerization. 1 Publication
Mutagenesisi105 – 1051C → A: Does not affect homooligomerization. 1 Publication
Mutagenesisi108 – 1081C → A: Does not affect homooligomerization. 1 Publication
Mutagenesisi144 – 1441C → A: Does not affect homooligomerization. 1 Publication
Mutagenesisi242 – 2421C → A: Dominent-negative mutant that abolishes homooligomerization and function in membrane repair. 1 Publication
Mutagenesisi313 – 3131C → A: Does not affect homooligomerization. 1 Publication
Mutagenesisi456 – 4561C → A: Does not affect homooligomerization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Tripartite motif-containing protein 72
PRO_0000278131Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi242 – 242Interchain1 Publication

Post-translational modificationi

Disulfide bond formation at Cys-242 occurs in case of membrane damage that cause the entry of the oxidized milieu of the extracellular space, resulting in homooligomerization.

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ1XH17.
PaxDbiQ1XH17.
PRIDEiQ1XH17.

PTM databases

PhosphoSiteiQ1XH17.

Expressioni

Tissue specificityi

Muscle-specific. Exclusively expressed in cardiac and skeletal muscle.1 Publication

Gene expression databases

BgeeiQ1XH17.
CleanExiMM_TRIM72.
GenevestigatoriQ1XH17.

Interactioni

Subunit structurei

Homooligomer; disulfide-linked. Interacts with DYSF and CAV3.2 Publications

Protein-protein interaction databases

BioGridi241694. 2 interactions.
DIPiDIP-60129N.
STRINGi10090.ENSMUSP00000079832.

Structurei

3D structure databases

ProteinModelPortaliQ1XH17.
SMRiQ1XH17. Positions 8-56, 278-470.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini271 – 475205B30.2/SPRY
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili135 – 16935 Reviewed prediction
Add
BLAST
Coiled coili204 – 23229 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the TRIM/RBCC family.
Contains 1 B30.2/SPRY domain.

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiNOG305247.
GeneTreeiENSGT00750000117341.
HOGENOMiHOG000234133.
HOVERGENiHBG098570.
InParanoidiQ6NX76.
KOiK12036.
OMAiQDRVLVC.
OrthoDBiEOG7VTDMR.
PhylomeDBiQ1XH17.
TreeFamiTF342569.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR008985. ConA-like_lec_gl_sf.
IPR006574. PRY.
IPR003877. SPRY_rcpt.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1XH17-1 [UniParc]FASTAAdd to Basket

« Hide

MSAAPGLLRQ ELSCPLCLQL FDAPVTAECG HSFCRACLIR VAGEPAADGT    50
VACPCCQAPT RPQALSTNLQ LSRLVEGLAQ VPQGHCEEHL DPLSIYCEQD 100
RTLVCGVCAS LGSHRGHRLL PAAEAQARLK TQLPQQKMQL QEACMRKEKT 150
VAVLEHQLVE VEETVRQFRG AVGEQLGKMR MFLAALESSL DREAERVRGD 200
AGVALRRELS SLNSYLEQLR QMEKVLEEVA DKPQTEFLMK FCLVTSRLQK 250
ILSESPPPAR LDIQLPVISD DFKFQVWKKM FRALMPALEE LTFDPSSAHP 300
SLVVSSSGRR VECSDQKAPP AGEDTRQFDK AVAVVAQQLL SQGEHYWEVE 350
VGDKPRWALG VMAADASRRG RLHAVPSQGL WLLGLRDGKI LEAHVEAKEP 400
RALRTPERPP ARIGLYLSFA DGVLAFYDAS NPDVLTPIFS FHERLPGPVY 450
PIFDVCWHDK GKNAQPLLLV GPEQEQA 477
Length:477
Mass (Da):52,817
Last modified:May 2, 2006 - v1
Checksum:iDFB52299E5FFB66B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB231474 mRNA. Translation: BAE93014.1.
BC067209 mRNA. Translation: AAH67209.1.
BC147789 mRNA. Translation: AAI47790.1.
BC158111 mRNA. Translation: AAI58112.1.
BC158120 mRNA. Translation: AAI58121.1.
CCDSiCCDS40148.1.
RefSeqiNP_001073401.1. NM_001079932.3.
XP_006508065.1. XM_006508002.1.
UniGeneiMm.389924.

Genome annotation databases

EnsembliENSMUST00000081042; ENSMUSP00000079832; ENSMUSG00000042828.
ENSMUST00000106248; ENSMUSP00000101855; ENSMUSG00000042828.
GeneIDi434246.
KEGGimmu:434246.
UCSCiuc009jxv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB231474 mRNA. Translation: BAE93014.1 .
BC067209 mRNA. Translation: AAH67209.1 .
BC147789 mRNA. Translation: AAI47790.1 .
BC158111 mRNA. Translation: AAI58112.1 .
BC158120 mRNA. Translation: AAI58121.1 .
CCDSi CCDS40148.1.
RefSeqi NP_001073401.1. NM_001079932.3.
XP_006508065.1. XM_006508002.1.
UniGenei Mm.389924.

3D structure databases

ProteinModelPortali Q1XH17.
SMRi Q1XH17. Positions 8-56, 278-470.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 241694. 2 interactions.
DIPi DIP-60129N.
STRINGi 10090.ENSMUSP00000079832.

PTM databases

PhosphoSitei Q1XH17.

Proteomic databases

MaxQBi Q1XH17.
PaxDbi Q1XH17.
PRIDEi Q1XH17.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000081042 ; ENSMUSP00000079832 ; ENSMUSG00000042828 .
ENSMUST00000106248 ; ENSMUSP00000101855 ; ENSMUSG00000042828 .
GeneIDi 434246.
KEGGi mmu:434246.
UCSCi uc009jxv.2. mouse.

Organism-specific databases

CTDi 493829.
MGIi MGI:3612190. Trim72.

Phylogenomic databases

eggNOGi NOG305247.
GeneTreei ENSGT00750000117341.
HOGENOMi HOG000234133.
HOVERGENi HBG098570.
InParanoidi Q6NX76.
KOi K12036.
OMAi QDRVLVC.
OrthoDBi EOG7VTDMR.
PhylomeDBi Q1XH17.
TreeFami TF342569.

Miscellaneous databases

NextBioi 409468.
PROi Q1XH17.
SOURCEi Search...

Gene expression databases

Bgeei Q1XH17.
CleanExi MM_TRIM72.
Genevestigatori Q1XH17.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProi IPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR008985. ConA-like_lec_gl_sf.
IPR006574. PRY.
IPR003877. SPRY_rcpt.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view ]
Pfami PF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view ]
PRINTSi PR01407. BUTYPHLNCDUF.
SMARTi SM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 1 hit.
PROSITEi PS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, PHOSPHATIDYLSERINE-BINDING, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DISULFIDE BOND AT CYS-242, MUTAGENESIS OF CYS-14; CYS-17; CYS-29; CYS-34; CYS-37; CYS-53; CYS-55; CYS-56; CYS-86; CYS-97; CYS-105; CYS-108; CYS-144; CYS-242; CYS-313 AND CYS-456.
    Strain: C57BL/6J.
    Tissue: Skeletal muscle.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain, Jaw and Limb.
  3. Cited for: FUNCTION.
  4. "MG53 regulates membrane budding and exocytosis in muscle cells."
    Cai C., Masumiya H., Weisleder N., Pan Z., Nishi M., Komazaki S., Takeshima H., Ma J.
    J. Biol. Chem. 284:3314-3322(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "Membrane repair defects in muscular dystrophy are linked to altered interaction between MG53, caveolin-3, and dysferlin."
    Cai C., Weisleder N., Ko J.-K., Komazaki S., Sunada Y., Nishi M., Takeshima H., Ma J.
    J. Biol. Chem. 284:15894-15902(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH DYSF AND CAV3.

Entry informationi

Entry nameiTRI72_MOUSE
AccessioniPrimary (citable) accession number: Q1XH17
Secondary accession number(s): B2RWH7, Q6NX76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: May 2, 2006
Last modified: July 9, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi