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Q1XH05 (HGL1B_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1b, chloroplastic

EC=3.2.1.182
Alternative name(s):
Beta-glucosidase 1b
Short name=Taglu1b
EC=3.2.1.21
Gene names
Name:GLU1B
OrganismTriticum aestivum (Wheat) [Complete proteome]
Taxonomic identifier4565 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeTriticum

Protein attributes

Sequence length569 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts in defense of young plant parts against pests via the production of hydroxamic acids from hydroxamic acid glucosides. Enzymatic activity is highly correlated with plant growth. The preferred substrate is DIMBOA-beta-D-glucoside. Ref.1 Ref.2

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose. Ref.1 Ref.2

(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O = 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose. Ref.1 Ref.2

(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O = 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose. Ref.1 Ref.2

Subunit structure

Homo- and heterohexamers. Ref.1 Ref.2

Subcellular location

Plastidchloroplast Potential.

Tissue specificity

Expressed in young seedlings early after germination. Ref.2

Developmental stage

Peak of expression 36 to 48 hours after imbibition. Ref.1

Miscellaneous

Wheat is a hexaploid with three different genomes that contains at least four genes coding for GLU1: GLU1A (AC Q1XIR9), GLU1B (AC Q1XH05), GLU1C (AC Q1XH04) and GLU1D (AC D5MTF8). The monomers can aggregate in diverse combinations, reflecting the several isozymes found in the native enzyme described in Ref.2. The hexameric structure is required for activity toward DIMBOA-beta-D-glucoside (Ref.1).

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Biophysicochemical properties

Kinetic parameters:

kcat is 214 sec(-1) with DIBOA-beta-D-glucoside as substrate (with recombinant enzyme). kcat is 1201 sec(-1) with DIMBOA-beta-D-glucoside as substrate (with recombinant enzyme). kcat is 128.2 sec(-1) with p-nitrophenyl beta-D-glucopyranoside as substrate (with recombinant enzyme).

KM=1.34 mM for DIBOA-beta-D-glucoside (with native hexamer) Ref.2

KM=1.44 mM for DIBOA-beta-D-glucoside (with recombinant enzyme)

KM=0.272 mM for DIMBOA-beta-D-glucoside (with native hexamer)

KM=0.29 mM for DIMBOA-beta-D-glucoside (with recombinant enzyme)

KM=2.02 mM for HBOA-beta-D-glucoside (with native hexamer)

KM=0.320 mM for HMBOA-beta-D-glucoside (with native hexamer)

KM=1.7 mM for p-nitrophenyl beta-D-glucopyranoside (with native hexamer)

KM=2.16 mM for p-nitrophenyl beta-D-glucopyranoside (with recombinant enzyme)

KM=1.78 mM for p-nitrophenyl beta-D-galactopyranoside (with native hexamer)

KM=3.11 mM for p-nitrophenyl beta-D-xyloside (with native hexamer)

KM=0.67 mM for p-nitrophenyl beta-D-fucoside (with native hexamer)

KM=0.240 mM for esculin (with native hexamer)

Vmax=1060 nmol/sec/mg enzyme with DIBOA-beta-D-glucoside as substrate (with native hexamer)

Vmax=4100 nmol/sec/mg enzyme with DIMBOA-beta-D-glucoside as substrate (with native hexamer)

Vmax=220 nmol/sec/mg enzyme with HBOA-beta-D-glucoside as substrate (with native hexamer)

Vmax=540 nmol/sec/mg enzyme with HMBOA-beta-D-glucoside as substrate (with native hexamer)

Vmax=520 nmol/sec/mg enzyme with p-nitrophenyl beta-D-glucopyranoside as substrate (with native hexamer)

Vmax=47 nmol/sec/mg enzyme with p-nitrophenyl beta-D-galactopyranoside as substrate (with native hexamer)

Vmax=35 nmol/sec/mg enzyme with p-nitrophenyl beta-D-xyloside as substrate (with native hexamer)

Vmax=1080 nmol/sec/mg enzyme with p-nitrophenyl beta-D-fucoside as substrate (with native hexamer)

Vmax=320 nmol/sec/mg enzyme with esculin as substrate (with native hexamer)

pH dependence:

Optimum pH is 5.5.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5050Chloroplast Ref.2
Chain51 – 5695194-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1b, chloroplastic
PRO_0000424098

Regions

Region319 – 3202Sulfate binding
Region511 – 5122Substrate binding

Sites

Active site2401Proton donor By similarity
Active site4561Nucleophile By similarity
Binding site921Substrate
Binding site2391Substrate
Binding site2431Substrate
Binding site5041Substrate

Amino acid modifications

Disulfide bond259 ↔ 265 Ref.1

Secondary structure

................................................................................. 569
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q1XH05 [UniParc].

Last modified May 2, 2006. Version 1.
Checksum: 44FAB6E047F86F85

FASTA56964,482
        10         20         30         40         50         60 
MALLAAATLN PTTHLSLRSR AGRNSENLWL RSTASSQKSK GRFCNLTIRA GTPSKPAEPI 

        70         80         90        100        110        120 
GPVFTKLKPW QIPKRDWFDK DFLFGASTSA YQIEGAWNED GKGPSTWDHF CHTYPERISD 

       130        140        150        160        170        180 
MTNGDVAANS YHLYEEDVKA LKDMGMKVYR FSISWSRILP DGTGKVNQAG IDYYNKLINS 

       190        200        210        220        230        240 
LIDNDIVPYV TIWHWDTPQA LEDKYGGFLN RQIVDDYKQF AEVCFKNFGD RVKNWFTFNE 

       250        260        270        280        290        300 
PHTYCCFSYG EGIHAPGRCS PGMDCAVPEG DSLREPYTAG HHILLAHAEA VQLFKARYNM 

       310        320        330        340        350        360 
HGDSKIGMAF DVMGYEPYQD SFLDDQARER SIDYNMGWFL EPVVRGDYPF SMRSLIGDRL 

       370        380        390        400        410        420 
PMFTKEEQEK LASSCDIMGL NYYTSRFSKH VDMSPDFTPT LNTDDAYASS ETTGSDGNDI 

       430        440        450        460        470        480 
GPITGTYWIY MYPKGLTDLL LIMKEKYGNP PVFITENGIA DVEGDESMPD PLDDWKRLDY 

       490        500        510        520        530        540 
LQRHISAVKD AIDQGADVRG HFTWGLIDNF EWSLGYSSRF GLVYIDKNDG NKRKLKKSAK 

       550        560 
WFSKFNSVPK PLLKTTNNNA TMTAASVSV 

« Hide

References

[1]"Molecular and structural characterization of hexameric beta-D-glucosidases in wheat and rye."
Sue M., Yamazaki K., Yajima S., Nomura T., Matsukawa T., Iwamura H., Miyamoto T.
Plant Physiol. 141:1237-1247(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE, SUBUNIT, X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 50-569 IN COMPLEX WITH DIMBOA, DISULFIDE BOND.
Strain: cv. Chinese Spring.
Tissue: Shoot.
[2]"Purification and characterization of a hydroxamic acid glucoside beta-glucosidase from wheat (Triticum aestivum L.) seedlings."
Sue M., Ishihara A., Iwamura H.
Planta 210:432-438(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 51-62, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, SUBUNIT, X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 50-569.
Strain: cv. Asakazekomugi.
[3]"Active-site architecture of benzoxazinone-glucoside beta-D-glucosidases in Triticeae."
Sue M., Nakamura C., Miyamoto T., Yajima S.
Plant Sci. 180:268-275(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 50-569 IN COMPLEX WITH DIMBOA OR INHIBITOR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB236422 mRNA. Translation: BAE92259.1.
UniGeneTa.24442.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2DGAX-ray1.80A50-569[»]
3AIQX-ray1.90A50-569[»]
3AIRX-ray2.00A50-569[»]
3AISX-ray2.20A50-569[»]
ProteinModelPortalQ1XH05.
SMRQ1XH05. Positions 61-551.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneQ1XH05.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ1XH05.

Entry information

Entry nameHGL1B_WHEAT
AccessionPrimary (citable) accession number: Q1XH05
Entry history
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: May 2, 2006
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries