Q1XG90 (LYS2_CRAVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysozyme 2
EC=3.2.1.17

Alternative name(s):
1,4-beta-N-acetylmuramidase 2
cv-lysozyme 2

Gene names

Name:

lysoz2

Organism

Crassostrea virginica (Eastern oyster)

Taxonomic identifier

6565 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Bivalvia › Pteriomorphia › Ostreoida › Ostreoidea › Ostreidae › Crassostrea

Protein attributes

Sequence length	135 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The main role of this lysozyme is in digestion. Has antibacterial activity against the Gram-positive bacterium P.cerevisiae and the Gram-negative bacteria E.coli and V.vulnificus. Shows some chitinase activity but no isopeptidase activity. Ref.1
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. Ref.1
Enzyme regulation	Activity decreased by 80% by addition of 0.01 M calcium, zinc or magnesium. Activity only decreased by 17% by addition of ammonium, and by 2% by addition of sodium. Ref.1
Subcellular location	Secreted.
Tissue specificity	Expressed in the epithelia of the basophil cells in the digestive tubules, but not in the epithelial cells lining the digestive ducts and stomach. Expressed at a much lower level in the style sac-midgut tissues. No expression detected in mantle, gills, labial palps or hemocytes. Ref.1
Sequence similarities	Belongs to the lysozyme type I family.
Biophysicochemical properties	pH dependence: Retains more than 90% of its maximum activity between pH 5.4 and 6.4 in the ionic strength range of I=0.005-0.01. In buffers of I=0.005 more than 75% of maximum activity is retained between pH 5.3 and 7.5. In buffers of I=0.02 more than 75% of maximum activity is retained between pH 5.3 and 6.5. Ref.1 Temperature dependence: Optimum temperature is 55 degrees Celsius. No decrease in activity was detected after incubation at 30 degrees Celsius for 30 minutes. No activity could be detected after incubation at 90 degrees Celsius for 30 minutes or 100 degrees Celsius for 10 minutes. Ref.1
Mass spectrometry	Molecular mass is 12984.6 Da from positions 19 - 135. Determined by MALDI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Antibiotic Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cytolysis Inferred from direct assay Ref.1. Source: UniProtKB defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	lysozyme activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Ref.1
	Chain	19 – 135	117	Lysozyme 2 Ref.1		PRO_0000280510

Sequences

	Sequence		Length	Mass (Da)
	Q1XG90 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: 20465553F0D0D1BB	FASTA	135	14,950
	10 20 30 40 50 60 MNFLILFCVV ASASVVYSSI SDQCLRCICE VESGCRAIGC HWDVYSNSCG YFQIKQGYWT 70 80 90 100 110 120 DCGSPGHSME SCADNYNCAS GCVRSYMDHY IKYNGCADTC ESYARMHNGG PNGCKSSHHH 130 ATDNYWRLVQ AKGCS

References

[1]

"A new lysozyme from the eastern oyster (Crassostrea virginica) indicates adaptive evolution of i-type lysozymes." Xue Q.-G., Itoh N., Schey K.L., Li Y.-L., Cooper R.K., La Peyre J.F. Cell. Mol. Life Sci. 64:82-95(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-105 AND 116-135, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, MASS SPECTROMETRY. Tissue: Digestive gland.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB252064 mRNA. Translation: BAE93114.1.
3D structure databases
ProteinModelPortal	Q1XG90.
SMR	Q1XG90. Positions 18-131.
ModBase	Search...
Protein family/group databases
CAZy	GH22. Glycoside Hydrolase Family 22.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008597. Destabilase. IPR023346. Lysozyme-like_dom. [Graphical view]
PANTHER	PTHR11195. PTHR11195. 1 hit.
Pfam	PF05497. Destabilase. 1 hit. [Graphical view]
SUPFAM	SSF53955. SSF53955. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LYS2_CRAVI

Accession

Primary (citable) accession number: Q1XG90

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	May 2, 2006
Last modified:	October 3, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)