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Q1XG90

- LYS2_CRAVI

UniProt

Q1XG90 - LYS2_CRAVI

Protein

Lysozyme 2

Gene

lysoz2

Organism
Crassostrea virginica (Eastern oyster)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    The main role of this lysozyme is in digestion. Has antibacterial activity against the Gram-positive bacterium P.cerevisiae and the Gram-negative bacteria E.coli and V.vulnificus. Shows some chitinase activity but no isopeptidase activity.1 Publication

    Catalytic activityi

    Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.1 Publication

    Enzyme regulationi

    Activity decreased by 80% by addition of 0.01 M calcium, zinc or magnesium. Activity only decreased by 17% by addition of ammonium, and by 2% by addition of sodium.1 Publication

    pH dependencei

    Retains more than 90% of its maximum activity between pH 5.4 and 6.4 in the ionic strength range of I=0.005-0.01. In buffers of I=0.005 more than 75% of maximum activity is retained between pH 5.3 and 7.5. In buffers of I=0.02 more than 75% of maximum activity is retained between pH 5.3 and 6.5.1 Publication

    Temperature dependencei

    Optimum temperature is 55 degrees Celsius. No decrease in activity was detected after incubation at 30 degrees Celsius for 30 minutes. No activity could be detected after incubation at 90 degrees Celsius for 30 minutes or 100 degrees Celsius for 10 minutes.1 Publication

    GO - Molecular functioni

    1. lysozyme activity Source: UniProtKB

    GO - Biological processi

    1. cytolysis Source: UniProtKB
    2. defense response to bacterium Source: UniProtKB-KW

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH22. Glycoside Hydrolase Family 22.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysozyme 2 (EC:3.2.1.17)
    Alternative name(s):
    1,4-beta-N-acetylmuramidase 2
    cv-lysozyme 2
    Gene namesi
    Name:lysoz2Imported
    OrganismiCrassostrea virginica (Eastern oyster)
    Taxonomic identifieri6565 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaBivalviaPteriomorphiaOstreoidaOstreoideaOstreidaeCrassostrea

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 18181 PublicationAdd
    BLAST
    Chaini19 – 135117Lysozyme 21 PublicationPRO_0000280510Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in the epithelia of the basophil cells in the digestive tubules, but not in the epithelial cells lining the digestive ducts and stomach. Expressed at a much lower level in the style sac-midgut tissues. No expression detected in mantle, gills, labial palps or hemocytes.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ1XG90.
    SMRiQ1XG90. Positions 18-131.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the lysozyme type I family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR008597. Destabilase.
    IPR023346. Lysozyme-like_dom.
    [Graphical view]
    PANTHERiPTHR11195. PTHR11195. 1 hit.
    PfamiPF05497. Destabilase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53955. SSF53955. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q1XG90-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNFLILFCVV ASASVVYSSI SDQCLRCICE VESGCRAIGC HWDVYSNSCG    50
    YFQIKQGYWT DCGSPGHSME SCADNYNCAS GCVRSYMDHY IKYNGCADTC 100
    ESYARMHNGG PNGCKSSHHH ATDNYWRLVQ AKGCS 135
    Length:135
    Mass (Da):14,950
    Last modified:May 2, 2006 - v1
    Checksum:i20465553F0D0D1BB
    GO

    Mass spectrometryi

    Molecular mass is 12984.6 Da from positions 19 - 135. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB252064 mRNA. Translation: BAE93114.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB252064 mRNA. Translation: BAE93114.1 .

    3D structure databases

    ProteinModelPortali Q1XG90.
    SMRi Q1XG90. Positions 18-131.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH22. Glycoside Hydrolase Family 22.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR008597. Destabilase.
    IPR023346. Lysozyme-like_dom.
    [Graphical view ]
    PANTHERi PTHR11195. PTHR11195. 1 hit.
    Pfami PF05497. Destabilase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53955. SSF53955. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A new lysozyme from the eastern oyster (Crassostrea virginica) indicates adaptive evolution of i-type lysozymes."
      Xue Q.-G., Itoh N., Schey K.L., Li Y.-L., Cooper R.K., La Peyre J.F.
      Cell. Mol. Life Sci. 64:82-95(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-105 AND 116-135, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, MASS SPECTROMETRY.
      Tissue: Digestive glandImported.

    Entry informationi

    Entry nameiLYS2_CRAVI
    AccessioniPrimary (citable) accession number: Q1XG90
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 2007
    Last sequence update: May 2, 2006
    Last modified: October 1, 2014
    This is version 23 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3