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Protein

Lysozyme 2

Gene

lysoz2

Organism
Crassostrea virginica (Eastern oyster)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The main role of this lysozyme is in digestion. Has antibacterial activity against the Gram-positive bacterium P.cerevisiae and the Gram-negative bacteria E.coli and V.vulnificus. Shows some chitinase activity but no isopeptidase activity.1 Publication

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.1 Publication

Enzyme regulationi

Activity decreased by 80% by addition of 0.01 M calcium, zinc or magnesium. Activity only decreased by 17% by addition of ammonium, and by 2% by addition of sodium.1 Publication

pH dependencei

Retains more than 90% of its maximum activity between pH 5.4 and 6.4 in the ionic strength range of I=0.005-0.01. In buffers of I=0.005 more than 75% of maximum activity is retained between pH 5.3 and 7.5. In buffers of I=0.02 more than 75% of maximum activity is retained between pH 5.3 and 6.5.1 Publication

Temperature dependencei

Optimum temperature is 55 degrees Celsius. No decrease in activity was detected after incubation at 30 degrees Celsius for 30 minutes. No activity could be detected after incubation at 90 degrees Celsius for 30 minutes or 100 degrees Celsius for 10 minutes.1 Publication

GO - Molecular functioni

  1. lysozyme activity Source: UniProtKB

GO - Biological processi

  1. cytolysis Source: UniProtKB
  2. defense response to bacterium Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH22. Glycoside Hydrolase Family 22.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysozyme 2 (EC:3.2.1.17)
Alternative name(s):
1,4-beta-N-acetylmuramidase 2
cv-lysozyme 2
Gene namesi
Name:lysoz2Imported
OrganismiCrassostrea virginica (Eastern oyster)
Taxonomic identifieri6565 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaBivalviaPteriomorphiaOstreoidaOstreoideaOstreidaeCrassostrea

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 18181 PublicationAdd
BLAST
Chaini19 – 135117Lysozyme 21 PublicationPRO_0000280510Add
BLAST

Expressioni

Tissue specificityi

Expressed in the epithelia of the basophil cells in the digestive tubules, but not in the epithelial cells lining the digestive ducts and stomach. Expressed at a much lower level in the style sac-midgut tissues. No expression detected in mantle, gills, labial palps or hemocytes.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ1XG90.
SMRiQ1XG90. Positions 18-131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the lysozyme type I family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR008597. Destabilase.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PANTHERiPTHR11195. PTHR11195. 1 hit.
PfamiPF05497. Destabilase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q1XG90-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNFLILFCVV ASASVVYSSI SDQCLRCICE VESGCRAIGC HWDVYSNSCG
60 70 80 90 100
YFQIKQGYWT DCGSPGHSME SCADNYNCAS GCVRSYMDHY IKYNGCADTC
110 120 130
ESYARMHNGG PNGCKSSHHH ATDNYWRLVQ AKGCS
Length:135
Mass (Da):14,950
Last modified:May 2, 2006 - v1
Checksum:i20465553F0D0D1BB
GO

Mass spectrometryi

Molecular mass is 12984.6 Da from positions 19 - 135. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB252064 mRNA. Translation: BAE93114.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB252064 mRNA. Translation: BAE93114.1.

3D structure databases

ProteinModelPortaliQ1XG90.
SMRiQ1XG90. Positions 18-131.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH22. Glycoside Hydrolase Family 22.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR008597. Destabilase.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PANTHERiPTHR11195. PTHR11195. 1 hit.
PfamiPF05497. Destabilase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "A new lysozyme from the eastern oyster (Crassostrea virginica) indicates adaptive evolution of i-type lysozymes."
    Xue Q.-G., Itoh N., Schey K.L., Li Y.-L., Cooper R.K., La Peyre J.F.
    Cell. Mol. Life Sci. 64:82-95(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-105 AND 116-135, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, MASS SPECTROMETRY.
    Tissue: Digestive glandImported.

Entry informationi

Entry nameiLYS2_CRAVI
AccessioniPrimary (citable) accession number: Q1XG90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: May 2, 2006
Last modified: January 7, 2015
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.