ID   ACSF_PORYE              Reviewed;         349 AA.
AC   Q1XDK9;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2006, sequence version 1.
DT   16-JUN-2009, entry version 21.
DE   RecName: Full=Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase;
DE            Short=Mg-protoporphyrin IX monomethyl ester oxidative cyclase;
DE            EC=1.14.13.81;
GN   Name=acsF;
OS   Porphyra yezoensis.
OG   Plastid; Chloroplast.
OC   Eukaryota; Rhodophyta; Bangiophyceae; Bangiales; Bangiaceae; Porphyra.
OX   NCBI_TaxID=2788;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=U-51; TISSUE=Gametophyte;
RA   Kunimoto M., Morishima K., Yoshikawa M., Fukuda S., Kobayashi T.,
RA   Kobayashi M., Okazaki T., Ohara I., Nakayama I.;
RT   "Whole genome sequence of Porphyra yezoensis chloroplast.";
RL   Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the formation of the isocyclic ring in
CC       chlorophyll biosynthesis. Mediates the cyclase reaction, which
CC       results in the formation of divinylprotochlorophyllide (Pchlide)
CC       characteristic of all chlorophylls from magnesium-protoporphyrin
CC       IX 13-monomethyl ester (MgPMME) (By similarity).
CC   -!- CATALYTIC ACTIVITY: Magnesium-protoporphyrin IX 13-monomethyl
CC       ester + NADPH + O(2) = 13(1)-hydroxy-magnesium-protoporphyrin IX
CC       13-monomethyl ester + NADP(+) + H(2)O.
CC   -!- CATALYTIC ACTIVITY: 13(1)-hydroxy-magnesium-protoporphyrin IX 13-
CC       monomethyl ester + NADPH + O(2) = 13(1)-oxo-magnesium-
CC       protoporphyrin IX 13-monomethyl ester + NADP(+) + 2 H(2)O.
CC   -!- CATALYTIC ACTIVITY: 13(1)-oxo-magnesium-protoporphyrin IX 13-
CC       monomethyl ester + NADPH + O(2) = divinylprotochlorophyllide +
CC       NADP(+) + 2 H(2)O.
CC   -!- COFACTOR: Iron (By similarity).
CC   -!- PATHWAY: Porphyrin biosynthesis; chlorophyll biosynthesis (light-
CC       independent).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the acsF family.
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DR   EMBL; AP006715; BAE92402.1; -; Genomic_DNA.
DR   RefSeq; YP_536959.1; -.
DR   GeneID; 3978978; -.
DR   BRENDA; 1.14.13.81; 257472.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005506; F:iron ion binding; IEA:HAMAP.
DR   GO; GO:0048529; F:magnesium-protoporphyrin IX monomethyl este...; IEA:HAMAP.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:HAMAP.
DR   HAMAP; MF_01840; -; 1.
DR   InterPro; IPR008434; AcsF.
DR   InterPro; IPR003251; Rubrerythrin.
DR   Pfam; PF02915; Rubrerythrin; 1.
DR   TIGRFAMs; TIGR02029; AcsF; 1.
PE   3: Inferred from homology;
KW   Chlorophyll biosynthesis; Chloroplast; Iron; Metal-binding; NADP;
KW   Oxidoreductase; Photosynthesis; Plastid.
FT   CHAIN         1    349       Magnesium-protoporphyrin IX monomethyl
FT                                ester [oxidative] cyclase.
FT                                /FTId=PRO_0000277208.
SQ   SEQUENCE   349 AA;  41532 MW;  4149118183E776B9 CRC64;
     MQTTINNGQT SSKETLLTPR FYTTDFEEMA NMDISGNQED FLAILEEFRA DYNSEHFIRD
     EEFNQSWSNL EHKTKSLFIE FLERSCTAEF SGFLLYKELS RKLKDRNPVI AECFLLMSRD
     EARHAGFLNK AIGDFNLSLD LGFLTKSRKY TFFSPKFIFY ATYLSEKIGY WRYITIYRHL
     EQHPEHRIYP IFRFFENWCQ DENRHGDFFA ALLKSQPHFL NDWKAKMWCR FFLLSVFATM
     YLNDFQRIDF YNAIGLDSRQ YDMQVIRKTN ESAARVFPVA LDVDNPKFFK YLDTCACDNR
     ALIDIDNNNS PLFIKSIVKI PLYFSLFANL LKIYLIKPID SKTVWNTVR
//