Reviewed, UniProtKB/Swiss-Prot Q1XDK9 (ACSF_PORYE)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81

Gene names

Name:

acsF

Encoded on

Plastid; Chloroplast

Organism

Porphyra yezoensis

Taxonomic identifier

2788 [NCBI]

Taxonomic lineage

Eukaryota › Rhodophyta › Bangiophyceae › Bangiales › Bangiaceae › Porphyra

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity. HAMAP MF_01840
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O. HAMAP MF_01840 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O. HAMAP MF_01840 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O. HAMAP MF_01840
Cofactor	Iron By similarity. HAMAP MF_01840
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis (light-independent). HAMAP MF_01840
Subcellular location	Plastid › chloroplast HAMAP MF_01840.
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Plastid
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	iron ion binding Inferred from electronic annotation. Source: HAMAP magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase HAMAP MF_01840		PRO_0000277208

Sequences

	Sequence		Length	Mass (Da)
	Q1XDK9-1 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: 4149118183E776B9	FASTA	349	41,532
	10 20 30 40 50 60 MQTTINNGQT SSKETLLTPR FYTTDFEEMA NMDISGNQED FLAILEEFRA DYNSEHFIRD 70 80 90 100 110 120 EEFNQSWSNL EHKTKSLFIE FLERSCTAEF SGFLLYKELS RKLKDRNPVI AECFLLMSRD 130 140 150 160 170 180 EARHAGFLNK AIGDFNLSLD LGFLTKSRKY TFFSPKFIFY ATYLSEKIGY WRYITIYRHL 190 200 210 220 230 240 EQHPEHRIYP IFRFFENWCQ DENRHGDFFA ALLKSQPHFL NDWKAKMWCR FFLLSVFATM 250 260 270 280 290 300 YLNDFQRIDF YNAIGLDSRQ YDMQVIRKTN ESAARVFPVA LDVDNPKFFK YLDTCACDNR 310 320 330 340 ALIDIDNNNS PLFIKSIVKI PLYFSLFANL LKIYLIKPID SKTVWNTVR

References

[1]

"Whole genome sequence of Porphyra yezoensis chloroplast." Kunimoto M., Morishima K., Yoshikawa M., Fukuda S., Kobayashi T., Kobayashi M., Okazaki T., Ohara I., Nakayama I. Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: U-51. Tissue: Gametophyte.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006715 Genomic DNA. Translation: BAE92402.1.
RefSeq	YP_536959.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3978978.
Enzyme and pathway databases
BRENDA	1.14.13.81. 257472.
Family and domain databases
HAMAP	MF_01840. AcsF. [Tree]
InterPro	IPR008434. AcsF. IPR009078. Ferritin/RR-like. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF_PORYE

Accession

Primary (citable) accession number: Q1XDK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	May 2, 2006
Last modified:	February 9, 2010
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)