Reviewed, UniProtKB/Swiss-Prot Q1XDF6 (ILVB_PORYE)

Names and origin

Protein names

Recommended name:
    Acetolactate synthase large subunit
      Short name=AHAS
    EC=2.2.1.6
Alternative name(s):
    Acetohydroxy-acid synthase large subunit
      Short name=ALS

Gene names

Name:

ilvB

Encoded on

Plastid; Chloroplast

Organism

Porphyra yezoensis

Taxonomic identifier

2788 [NCBI]

Taxonomic lineage

Eukaryota › Rhodophyta › Bangiophyceae › Bangiales › Bangiaceae › Porphyra

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 pyruvate = 2-acetolactate + CO2.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.
Subunit structure	Dimer of large and small chains By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Cellular component	Chloroplast Plastid
Ligand	FAD Flavoprotein Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro acetolactate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	Acetolactate synthase large subunit		PRO_0000277306
Regions
	Nucleotide binding	271 – 292	22	FAD By similarity
	Nucleotide binding	314 – 333	20	FAD By similarity
	Region	405 – 484	80	Thiamine pyrophosphate binding
Sites
	Metal binding	455	1	Magnesium By similarity
	Metal binding	482	1	Magnesium By similarity
	Binding site	61	1	Thiamine pyrophosphate By similarity
	Binding site	163	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1XDF6-1 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: A2688F3D9B575511	FASTA	590	64,684
	10 20 30 40 50 60 MPLKQRVSSE KTGAFALLDS IVRHGVKHIF GYPGGAILPI YDELYAWEEA SLIKHILVRH 70 80 90 100 110 120 EQGAAHAADS YSRSTGEVGV CFATSGPGAT NLVSGIATAH IDSVPILAIT GQVGRAFIGT 130 140 150 160 170 180 DAFQEVDIFG ITLPIVKHSY VVRDPRDMSR IVAEAFFICK HGRPGPVLID VPKDVGLEKF 190 200 210 220 230 240 NYFSVEPGKV NIPGCRPITS LKSRQILMAA KMIQQSSQPL LYIGGGAIIS DSHQIIKELV 250 260 270 280 290 300 DFYKIPVTTT LMGKGIFNED SDYCLGMLGM HGTAYANFAV SECDLLIALG ARFDDRVTGK 310 320 330 340 350 360 LDEFACNAQV IHVDIDPAEV GKNRIPQVAI VGDVAEVVSE ILNLLKTSFP PYPEQIISWQ 370 380 390 400 410 420 ERINRWRQQY PLLVPRKSTS ISPQVILVAT NKLAQNAYFT TDVGQHQMWS AQFLKVKAKH 430 440 450 460 470 480 WLSSAGLGTM GYGLPAAIGA QVAHPNDVVI CISGDSSFQM NMQELGTIAQ YQLPVKIIII 490 500 510 520 530 540 NNRWQGMVRQ WQQAFYGERY SHSRMTEGAP DFQKLAEAFG IKAFTINNRQ NMQSALQVAI 550 560 570 580 590 DYPGPVLLDC QVTENENCYP MVAPGKSNAQ MIGIAKPQRG TASNYINNSV

References

[1]

"Whole genome sequence of Porphyra yezoensis chloroplast." Kunimoto M., Morishima K., Yoshikawa M., Fukuda S., Kobayashi T., Kobayashi M., Okazaki T., Ohara I., Nakayama I. Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: U-51. Tissue: Gametophyte.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006715 Genomic DNA. Translation: BAE92455.1.
RefSeq	YP_537012.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3978889.
Enzyme and pathway databases
BRENDA	2.2.1.6. 257472.
Family and domain databases
InterPro	IPR012846. Acetolactate_synth_lsu. IPR000399. TPP_bd_CS. IPR012001. TPP_bd_enzyme_N. IPR011766. TPP_enzyme_bd_C. IPR012000. TPP_enzyme_M. [Graphical view]
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00118. acolac_lg. 1 hit.
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVB_PORYE

Accession

Primary (citable) accession number: Q1XDF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	May 2, 2006
Last modified:	June 16, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)