Q1XDF3 (DNAB_PORYE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable replicative DNA helicase
EC=3.6.4.12

Cleaved into the following chain:

1. Pye dnaB intein

Gene names

Name:

dnaB

Encoded on

Plastid; Chloroplast

Organism

Porphyra yezoensis

Taxonomic identifier

2788 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Rhodophyta › Bangiophyceae › Bangiales › Bangiaceae › Pyropia

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + H2O = ADP + phosphate.
Subcellular location	Plastid › chloroplast.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the helicase family. DnaB subfamily. Contains 1 SF4 helicase domain.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Chloroplast Plastid Primosome
Domain	Repeat
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Autocatalytic cleavage Protein splicing
Gene Ontology (GO)
Biological_process	DNA duplex unwinding Inferred from electronic annotation. Source: GOC DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell replisome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Probable replicative DNA helicase, 1st part Potential		PRO_0000277258
	Chain	362 – 509	148	Pye dnaB intein Potential		PRO_0000277259
	Chain	510 – 566	57	Probable replicative DNA helicase, 2nd part Potential		PRO_0000277260
Regions
	Domain	156 – 426	271	SF4 helicase; first part
	Domain	308 – 566	259	SF4 helicase; second part
	Nucleotide binding	187 – 194	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1XDF3 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: C557181FDB577549	FASTA	566	64,847
	10 20 30 40 50 60 MLTQQSISLL KSVEKISPDF FYFTSTSLLY RAALENVNHA KTKSVRNFFI NLKNEKMIQD 70 80 90 100 110 120 LNELNEVFSL IEQAPLSDTI SEYSAVVIDN YIKRLLLACG DSLCLISCSK QLIEQKNITS 130 140 150 160 170 180 IVSQLTKAYE ILDEKDTQTL ATILANLLVH LDTRKKISIN SSILSGFTEL DSITQGFKKS 190 200 210 220 230 240 DLIILAGRPS MGKTAFAINI TRYVINQKRS YVILFSLEMS TEQLLRRILA QECNLNGQKI 250 260 270 280 290 300 QSGQLNNDEW QYVIQKSKTL ANLNLYIDDS AKISTDTIKT KVKFFKLQGK NIELIIIDYL 310 320 330 340 350 360 QLLQESRQSD NRSQELSLIT RSLKILAKDL SLPILALSQL NRNLETRSDK RPLLSDLRES 370 380 390 400 410 420 GCISKFNYLQ TSLYNQLQIL FNCYYKKIEV INFSAQKQQL FLAIKCSISK TGKKTVYKII 430 440 450 460 470 480 TEAGKYIQLT SNHKLLTTQG WKRCDKINQN DMIAIQIKNF EEQKNVFNSF SSLTFESIQK 490 500 510 520 530 540 INVTSLQIVF DLECKPLCNF ISNNFIVHNS IEQDADLVIM LYRESYYTQE TRSRDFTEVI 550 560 IAKHRNGPTG TFQLKFNAQI AKFSDA

References

[1]

"Whole genome sequence of Porphyra yezoensis chloroplast." Kunimoto M., Morishima K., Yoshikawa M., Fukuda S., Kobayashi T., Kobayashi M., Okazaki T., Ohara I., Nakayama I. Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: U-51.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006715 Genomic DNA. Translation: BAE92458.1.
RefSeq	YP_537015.1. NC_007932.1.
3D structure databases
ProteinModelPortal	Q1XDF3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3978882.
Phylogenomic databases
ProtClustDB	CLSC858394.
Family and domain databases
InterPro	IPR007692. DNA_helicase_DnaB. IPR007694. DNA_helicase_DnaB-like_C. IPR007693. DNA_helicase_DnaB-like_N. IPR006141. Intein_splice_site. [Graphical view]
Pfam	PF03796. DnaB_C. 2 hits. [Graphical view]
SUPFAM	SSF48024. DNA_helicase_DnaB-like_N. 1 hit.
TIGRFAMs	TIGR00665. DnaB. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50817. INTEIN_N_TER. False negative. PS51199. SF4_HELICASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAB_PORYE

Accession

Primary (citable) accession number: Q1XDF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	May 2, 2006
Last modified:	March 6, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)