Reviewed, UniProtKB/Swiss-Prot Q1WVB1 (PYRC_LACS1)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	LSL_0191

Organism

Lactobacillus salivarius subsp. salivarius (strain UCC118) [Complete proteome] [HAMAP]

Taxonomic identifier

362948 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Dihydroorotase		PRO_1000024091
Sites
	Metal binding	61	1	Zinc 1 By similarity
	Metal binding	63	1	Zinc 1 By similarity
	Metal binding	143	1	Zinc 1; via carbamate group By similarity
	Metal binding	143	1	Zinc 2; via carbamate group By similarity
	Metal binding	180	1	Zinc 2 By similarity
	Metal binding	233	1	Zinc 2 By similarity
	Metal binding	306	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	143	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1WVB1-1 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: 7A32DF5D16BE2303	FASTA	429	46,735
	10 20 30 40 50 60 MATLIKNGNL YQAGRIYQAD VLIEDGKIKA IGKNLSDVVK ENLVEIDATN KLVAPGLVDV 70 80 90 100 110 120 HVHYRDPGFT YKETIHTGSL AAAHGGYTTV CAMPNLDPVP DTPELVEKMC SRNQEDGVVK 130 140 150 160 170 180 VKQYGSITHG LKSEELVDFV GMKKAGAFAF SNDGSGVQTA GTMYQAMKSA AALNMAIVAH 190 200 210 220 230 240 VEDNSLLFGG VMNAGKRADE LGLPGILGIS ESSQIARDLL LAKETGVHYH VCHVSTKESV 250 260 270 280 290 300 ELVRLAKQHK INVTCEVSPH HLLLADVDIP GNDPYYKMNP PLRGVEDRQA LIDSLLDGTI 310 320 330 340 350 360 DMIATDHAPH SIDEKTGDMR EASFGITGSE TAFAMLYTKF VKTGIFTLEQ LLQWMSINPA 370 380 390 400 410 420 EKFGMEDAGE LLPGKSADLA IFDLNKEYVI KEEDYLSKGI NTPFTGEKVY GQTVLTMVDG QKVYQREEK

References

[1]

"Multireplicon genome architecture of Lactobacillus salivarius." Claesson M.J., Li Y., Leahy S., Canchaya C., van Pijkeren J.P., Cerdeno-Tarraga A.M., Parkhill J., Flynn S., O'Sullivan G.C., Collins J.K., Higgins D., Shanahan F., Fitzgerald G.F., van Sinderen D., O'Toole P.W. Proc. Natl. Acad. Sci. U.S.A. 103:6718-6723(2006) [PubMed: 16617113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000233 Genomic DNA. Translation: ABD99006.1.
RefSeq	YP_535089.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M38.972.
Genome annotation databases
GeneID	3978041.
GenomeReviews	Gene locus LSL_0191 in contig CP000233_GR.
KEGG	lsl:LSL_0191.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1WVB1.
Enzyme and pathway databases
BioCyc	LSAL362948:LSL_0191-MON.
Family and domain databases
HAMAP	MF_00220. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOmult. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD000518. DHOase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00857. pyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. False negative. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_LACS1

Accession

Primary (citable) accession number: Q1WVB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 2, 2006
Last modified:	September 2, 2008
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)