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Q1WUT3 (SYL_LACS1) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:LSL_0442
OrganismLactobacillus salivarius (strain UCC118) [Complete proteome] [HAMAP]
Taxonomic identifier362948 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length805 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 805805Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009361

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1WUT3 [UniParc].

Last modified May 2, 2006. Version 1.
Checksum: 255216A43873B93C

FASTA80592,215
        10         20         30         40         50         60 
MSYKHIEIEK KWQRYWEEHK TFKTTEDDDK KNYYALDMFP YPSGQGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
MARMKRMQGY NVLHPMGWDA FGLPAEQYAL NTGNSPREFT KKNVNNFRRQ IKSLGLSYDW 

       130        140        150        160        170        180 
DREVNTTDPA YYKWTQWIFE QLYKKGLAYE AEVPVNWSPD LGTVVANEEV IDGKTERGGF 

       190        200        210        220        230        240 
PVIRKPMRQW VLKITAYADR LIDDLDDLDW PEAIKEQQRN WIGRSVGAAI NFPVSGDENT 

       250        260        270        280        290        300 
KIEVFSTRPD TIFGVAALVL APEHELVKQL TTPEHENEVE AYIEKISHKS DLERTDLAKD 

       310        320        330        340        350        360 
KTGVFTGSYV VNPVSGEKLP IWIADYVLNS YGTGAVMVVP AHDERDHEFA QKFDLPIVQV 

       370        380        390        400        410        420 
IEGGDVQKEA YTGDGVHINS DFLNGMDKEE AIDAINNWLE ENGVGEKKVN YRLRDWLFSR 

       430        440        450        460        470        480 
QRYWGEPIPV IHWEDGETTL VPEDELPLYL PKATDIKPSG TGESPLANLD DWVNVVDENG 

       490        500        510        520        530        540 
RKGRRETNTM PQWAGSSWYF LRYIDPHNNH EIADYEKLKE WLPVNLYVGG AEHAVLHLLY 

       550        560        570        580        590        600 
ARFWHKFLYD LGVVPTKEPF QKLVNQGMIL GSNHEKMSKS KGNVVNPDDI VEQYGADTLR 

       610        620        630        640        650        660 
LYEMFMGPLD ASIPWSEEGL GGAHKFINRV WNLLIDENDN LRDRVTTINN HDLDKIYNET 

       670        680        690        700        710        720 
VKKVTEDYEA MHFNTAISQL MVFVNNAYKA DSLPLEYVEG LVKLLSPVVP HITEELWSKL 

       730        740        750        760        770        780 
GHVGSIAYAK WPTYDESKLV EDVVEIVVQI NGKVRQHLQV SKDASREELQ ALALNDERIK 

       790        800 
QELADKEVKK VIAVPGKLVS IVVAK 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000233 Genomic DNA. Translation: ABD99252.1.
RefSeqYP_535335.1. NC_007929.1.

3D structure databases

ProteinModelPortalQ1WUT3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING362948.LSL_0442.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD99252; ABD99252; LSL_0442.
GeneID3977437.
KEGGlsl:LSL_0442.
PATRIC22277779. VBILacSal43464_0518.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMADKPKYYA.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycLSAL362948:GJDJ-507-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LACS1
AccessionPrimary (citable) accession number: Q1WUT3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 2, 2006
Last modified: June 11, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries