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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Lactobacillus salivarius (strain UCC118)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciLSAL362948:GJDJ-794-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gid
Ordered Locus Names:LSL_0721
OrganismiLactobacillus salivarius (strain UCC118)
Taxonomic identifieri362948 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000006559 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 436436Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_1000072978Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi362948.LSL_0721.

Structurei

3D structure databases

ProteinModelPortaliQ1WU04.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiPOG091H00B3.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1WU04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTASVNVIGA GLAGSEAAWQ IANQGVKVRL YEMRPQKLTP AHHNENFAEL
60 70 80 90 100
VCTNSLRANR LTNAAGLLKE EMRTFNSIIM ESADKHSVPA GGALAVDRET
110 120 130 140 150
FSKEVTEKLH NHPNVEIINE EIDEIPEGLT VIATGPLTSD ALAKDITKFT
160 170 180 190 200
GSDGLFFFDA AAPILEKSSL DMDKVYLKSR YDKGEAAYLN APMTKDEFYN
210 220 230 240 250
FYNELIKAET AELHDFEDDK FFEGCMPIEE IASRGAQTML YGPLKPVGLE
260 270 280 290 300
DPRTGKEPFA VVQLRQDNAA GDLYNIVGFQ THLKWGEQKR VFSMIPGLEN
310 320 330 340 350
ARFVRYGVMH RNTFLCSPEV MQATYQTKKR LDLFFAGQMT GVEGYVESAA
360 370 380 390 400
SGLYAGLNAA RIAQGKDPVI FPEETMMGAM AHYITHASVK NFQPINANFG
410 420 430
IVPKLQERIR NKQERNLKIS ERAIDRIKKF KNLNFD
Length:436
Mass (Da):48,676
Last modified:May 2, 2006 - v1
Checksum:i01571FADDDE6DB3C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000233 Genomic DNA. Translation: ABD99531.1.
RefSeqiWP_011475886.1. NC_007929.1.
YP_535614.1. NC_007929.1.

Genome annotation databases

EnsemblBacteriaiABD99531; ABD99531; LSL_0721.
GeneIDi3976950.
KEGGilsl:LSL_0721.
PATRICi22278351. VBILacSal43464_0800.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000233 Genomic DNA. Translation: ABD99531.1.
RefSeqiWP_011475886.1. NC_007929.1.
YP_535614.1. NC_007929.1.

3D structure databases

ProteinModelPortaliQ1WU04.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi362948.LSL_0721.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD99531; ABD99531; LSL_0721.
GeneIDi3976950.
KEGGilsl:LSL_0721.
PATRICi22278351. VBILacSal43464_0800.

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiPOG091H00B3.

Enzyme and pathway databases

BioCyciLSAL362948:GJDJ-794-MONOMER.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMFO_LACS1
AccessioniPrimary (citable) accession number: Q1WU04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 2, 2006
Last modified: September 7, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.