Reviewed, UniProtKB/Swiss-Prot Q1WU04 (TRMFO_LACS1)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO
    EC=2.1.1.74
Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferase
    Folate-dependent tRNA(M-5-U54)-methyltransferase

Gene names

Name:	trmFO
Synonyms:	gid
Ordered Locus Names:	LSL_0721

Organism

Lactobacillus salivarius subsp. salivarius (strain UCC118) [Complete proteome] [HAMAP]

Taxonomic identifier

362948 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC tRNA (uracil-5-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO HAMAP MF_01037		PRO_1000072978
Regions
	Nucleotide binding	9 – 14	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1WU04-1 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: 01571FADDDE6DB3C	FASTA	436	48,676
	10 20 30 40 50 60 MTASVNVIGA GLAGSEAAWQ IANQGVKVRL YEMRPQKLTP AHHNENFAEL VCTNSLRANR 70 80 90 100 110 120 LTNAAGLLKE EMRTFNSIIM ESADKHSVPA GGALAVDRET FSKEVTEKLH NHPNVEIINE 130 140 150 160 170 180 EIDEIPEGLT VIATGPLTSD ALAKDITKFT GSDGLFFFDA AAPILEKSSL DMDKVYLKSR 190 200 210 220 230 240 YDKGEAAYLN APMTKDEFYN FYNELIKAET AELHDFEDDK FFEGCMPIEE IASRGAQTML 250 260 270 280 290 300 YGPLKPVGLE DPRTGKEPFA VVQLRQDNAA GDLYNIVGFQ THLKWGEQKR VFSMIPGLEN 310 320 330 340 350 360 ARFVRYGVMH RNTFLCSPEV MQATYQTKKR LDLFFAGQMT GVEGYVESAA SGLYAGLNAA 370 380 390 400 410 420 RIAQGKDPVI FPEETMMGAM AHYITHASVK NFQPINANFG IVPKLQERIR NKQERNLKIS 430 ERAIDRIKKF KNLNFD

References

[1]

"Multireplicon genome architecture of Lactobacillus salivarius." Claesson M.J., Li Y., Leahy S., Canchaya C., van Pijkeren J.P., Cerdeno-Tarraga A.M., Parkhill J., Flynn S., O'Sullivan G.C., Collins J.K., Higgins D., Shanahan F., Fitzgerald G.F., van Sinderen D., O'Toole P.W. Proc. Natl. Acad. Sci. U.S.A. 103:6718-6723(2006) [PubMed: 16617113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000233 Genomic DNA. Translation: ABD99531.1.
RefSeq	YP_535614.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3976950.
GenomeReviews	Gene locus LSL_0721 in contig CP000233_GR.
KEGG	lsl:LSL_0721.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1WU04.
OMA	Q1WU04. MKPVGLT.
Enzyme and pathway databases
BioCyc	LSAL362948:LSL_0721-MON.
Family and domain databases
HAMAP	MF_01037. [Tree]
InterPro	IPR004417. Gid. IPR002218. GIDA-rel. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11806. GIDA. 1 hit.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
ProDom	PD003738. GIDA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00137. gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMFO_LACS1

Accession

Primary (citable) accession number: Q1WU04

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 2, 2006
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)