ID   FTHS_LIGS1              Reviewed;         552 AA.
AC   Q1WTW0;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2006, sequence version 1.
DT   27-MAR-2024, entry version 89.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=LSL_0842;
OS   Ligilactobacillus salivarius (strain UCC118) (Lactobacillus salivarius).
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Ligilactobacillus.
OX   NCBI_TaxID=362948;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCC118;
RX   PubMed=16617113; DOI=10.1073/pnas.0511060103;
RA   Claesson M.J., Li Y., Leahy S., Canchaya C., van Pijkeren J.P.,
RA   Cerdeno-Tarraga A.M., Parkhill J., Flynn S., O'Sullivan G.C., Collins J.K.,
RA   Higgins D., Shanahan F., Fitzgerald G.F., van Sinderen D., O'Toole P.W.;
RT   "Multireplicon genome architecture of Lactobacillus salivarius.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:6718-6723(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000233; ABD99652.1; -; Genomic_DNA.
DR   RefSeq; WP_011475989.1; NC_007929.1.
DR   RefSeq; YP_535735.1; NC_007929.1.
DR   AlphaFoldDB; Q1WTW0; -.
DR   SMR; Q1WTW0; -.
DR   STRING; 362948.LSL_0842; -.
DR   KEGG; lsl:LSL_0842; -.
DR   PATRIC; fig|362948.14.peg.916; -.
DR   HOGENOM; CLU_003601_3_3_9; -.
DR   OrthoDB; 9761733at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000006559; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..552
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000293043"
FT   BINDING         62..69
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   552 AA;  59469 MW;  60151621C7ABA759 CRC64;
     MKDIEIAQKS EMLPITEVAQ KVGLTADEIE QYGKYKAKIS LPLSAREQTK RKLVLVTAIN
     PTPAGEGKST VTVGLGDAFS LLNKKVMIAL REPSLGPVMG MKGGATGGGY SQVVPMEDIN
     LHFTGDMHAL TTANNTLAAL IDNHIYQGNK LNIDQRRVIW KRVLDINDRA LRHVVIGLGG
     ATQGIPREDG FDITVASELM AILCLSKDIS DLKKRIGQIV IGYNFDKEPV TVDQLGVTGA
     ITLLLKDACK PNLVQTLAHT PAIVHGGPFA NIAHGCNSVL ATQTALNLAD YTITEAGFGA
     DLGAEKFLDI KTPVLGKTPD TIVIVATARA LKMNGGVAKD NLDEENVAAV EQGFANLKKH
     IQSMKRYNVP VVVAINKFTQ DTDAELAKIV ELCEQDDTKA IVADVWAKGG EGATELAKAV
     IESCDNDQEF TRLYNSDDSV EEKINKIVTE IYGGDGVEFS AKAKRQLRQF EKLGWNHLPV
     CMAKTQYSLS DNAKVLGAPK GFKIHVREFV PKLGAQFLVA LTGNILTMPG LPKVPAADGM
     DVDENGKISG LY
//