Q1WTU1 (Q1WTU1_LACS1) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase HAMAP MF_01684
Short name=MTA/SAH nucleosidase HAMAP MF_01684
Short name=MTAN HAMAP MF_01684
EC=3.2.2.9 HAMAP MF_01684

Alternative name(s):
5'-methylthioadenosine nucleosidase HAMAP MF_01684
S-adenosylhomocysteine nucleosidase HAMAP MF_01684

Gene names

Name:	pfs EMBL ABD99669.1
Synonyms:	mtnN HAMAP MF_01684
Ordered Locus Names:	LSL_0859

Organism

Lactobacillus salivarius (strain UCC118) [Complete proteome] [HAMAP] EMBL ABD99669.1

Taxonomic identifier

362948 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively By similarity. HAMAP MF_01684
Catalytic activity	S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine. HAMAP MF_01684 SAAS SAAS010049 S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine. HAMAP MF_01684 SAAS SAAS010049
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2. HAMAP MF_01684
Sequence similarities	Belongs to the PNP/UDP phosphorylase family. MtnN subfamily. HAMAP MF_01684

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis HAMAP MF_01684 SAAS SAAS010049
Molecular function	Glycosidase EMBL ABD99669.1 Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-methionine biosynthetic process from S-adenosylmethionine Inferred from electronic annotation. Source: HAMAP L-methionine salvage from methylthioadenosine Inferred from electronic annotation. Source: InterPro nucleoside catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenosylhomocysteine nucleosidase activity Inferred from electronic annotation. Source: HAMAP methylthioadenosine nucleosidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	173 – 174	2	Substrate binding By similarity HAMAP MF_01684
Sites
	Active site	12	1	Proton acceptor By similarity HAMAP MF_01684
	Binding site	78	1	Substrate; via amide nitrogen By similarity HAMAP MF_01684
	Binding site	197	1	Substrate By similarity HAMAP MF_01684

Sequences

	Sequence		Length	Mass (Da)
	Q1WTU1 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: ECF0C2224E613086	FASTA	228	24,106
	10 20 30 40 50 60 MKYGIICAME EEIKSLVAQL EDKKQDTIGG IDFYSGTING KEVVLTRSGI GKVQAGVTTG 70 80 90 100 110 120 LLIANYKVDA VINSGSAGGI GEGLHVGDVV LSTGAAYHDA DATAFGYKPG QLPQQPQIYT 130 140 150 160 170 180 ADKDLLEAIS KAAKESNLVA KEGLIVTGDQ FVSSSEKIAA IKEIYPDALS CEMEGAAIAQ 190 200 210 220 VAYQFSVPFL IIRAMSDVGD EDAGQSFDEF IIEAGKKSAN MILNFLKK

References

[1]

"Multireplicon genome architecture of Lactobacillus salivarius." Claesson M.J., Li Y., Leahy S., Canchaya C., van Pijkeren J.P., Cerdeno-Tarraga A.M., Parkhill J., Flynn S., O'Sullivan G.C., Collins J.K., Higgins D., Shanahan F., Fitzgerald G.F., van Sinderen D., O'Toole P.W. Proc. Natl. Acad. Sci. U.S.A. 103:6718-6723(2006) [PubMed: 16617113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UCC118.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000233 Genomic DNA. Translation: ABD99669.1.
RefSeq	YP_535752.1. NC_007929.1.
3D structure databases
ProteinModelPortal	Q1WTU1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1WTU1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3977752.
GenomeReviews	Gene locus LSL_0859 in contig CP000233_GR.
KEGG	lsl:LSL_0859.
PATRIC	22278623. VBILacSal43464_0934.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0775.
HOGENOM	HBG367723.
OMA	LEHFPTM.
PhylomeDB	Q1WTU1.
ProtClustDB	CLSK770861.
Family and domain databases
HAMAP	MF_01684. Salvage_tnN. [Tree]
InterPro	IPR010049. MTA_SAH_Nsdase. IPR018017. Nucleoside_phosphorylase. IPR000845. Nucleoside_phosphorylase_d. [Graphical view]
KO	K01243.
PANTHER	PTHR21234. PNP_UDP. 1 hit. PTHR21234:SF6. PTHR21234:SF6. 1 hit.
Pfam	PF01048. PNP_UDP_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01704. MTA/SAH-Nsdase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q1WTU1_LACS1

Accession

Primary (citable) accession number: Q1WTU1

Entry history

Integrated into UniProtKB/TrEMBL:	May 2, 2006
Last sequence update:	May 2, 2006
Last modified:	December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)