Reviewed, UniProtKB/Swiss-Prot Q1WTD6 (ASNA_LACS1)

Names and origin

Protein names

Recommended name:
    Aspartate--ammonia ligase
    EC=6.3.1.1
Alternative name(s):
    Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	LSL_1033

Organism

Lactobacillus salivarius subsp. salivarius (strain UCC118) [Complete proteome] [HAMAP]

Taxonomic identifier

362948 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000017953

Sequences

	Sequence		Length	Mass (Da)
	Q1WTD6-1 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: C296A67F4BF5C4CF	FASTA	336	39,133
	10 20 30 40 50 60 MDLIIPKDYD PKLSIRETQE AIRYIRETFQ DEFGKEMGLN RVSAPMYVEK SSGINDNLNG 70 80 90 100 110 120 YEKPVSFTMK DMPGETIEVV HSLAKWKRMA LKKYGFGLHE GLYTNMNAIR KDEDLDNFHS 130 140 150 160 170 180 SYVDQWDWEK VISKDERNEK TLKETVELIF KVVKHMEHEV WYKFPNAVYH LPDKIHFITS 190 200 210 220 230 240 QELEDKYPEL EDAKDRENAI CKELGCVFVM QIGDVLKSGK RHDGRAPDYD DWKLNGDILF 250 260 270 280 290 300 WYEPLQCALE LSSMGIRVDE DSMVEQLKKT GDEDRLKLQY HKMILNKELP YTIGGGIGQS 310 320 330 RLCMLLLGKA HVGEVQASIW PDEMLKKCEE NGIHIL

References

[1]

"Multireplicon genome architecture of Lactobacillus salivarius." Claesson M.J., Li Y., Leahy S., Canchaya C., van Pijkeren J.P., Cerdeno-Tarraga A.M., Parkhill J., Flynn S., O'Sullivan G.C., Collins J.K., Higgins D., Shanahan F., Fitzgerald G.F., van Sinderen D., O'Toole P.W. Proc. Natl. Acad. Sci. U.S.A. 103:6718-6723(2006) [PubMed: 16617113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000233 Genomic DNA. Translation: ABD99841.1.
RefSeq	YP_535924.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1WTD6.
Genome annotation databases
GeneID	3977019.
GenomeReviews	Gene locus LSL_1033 in contig CP000233_GR.
KEGG	lsl:LSL_1033.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2502.
HOGENOM	HBG288146.
OMA	LNDNLNG.
PhylomeDB	Q1WTD6.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-dom. IPR004618. AsnA. [Graphical view]
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. asnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_LACS1

Accession

Primary (citable) accession number: Q1WTD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 2, 2006
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)