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Q1WTA9

- SYI_LACS1

UniProt

Q1WTA9 - SYI_LACS1

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus salivarius (strain UCC118)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei556 – 5561Aminoacyl-adenylateUniRule annotation
Binding sitei600 – 6001ATPUniRule annotation
Metal bindingi891 – 8911ZincUniRule annotation
Metal bindingi894 – 8941ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation
Metal bindingi914 – 9141ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLSAL362948:GJDJ-1120-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:LSL_1042
OrganismiLactobacillus salivarius (strain UCC118)
Taxonomic identifieri362948 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000006559: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 931931Isoleucine--tRNA ligasePRO_1000022085Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi362948.LSL_1042.

Structurei

3D structure databases

ProteinModelPortaliQ1WTA9.
SMRiQ1WTA9. Positions 1-920.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi597 – 6015"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1WTA9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVKDTLNLG KTKFPMRGNL PVREVERQNE WEENKVYEQR QKLNEGKPSF
60 70 80 90 100
VLHDGPPYAN GNIHMGHAMN KISKDFIVRS KSMMGFRAPY VPGWDTHGLP
110 120 130 140 150
IEQQLKKAGV DRKALSVAEF REMCRQYALE QVDKQRKDFK RLGVAGEWDN
160 170 180 190 200
PYLTLKPEYE AQQIRVFGKM AEKGLIYKGK KPVFWSWSSE SALAEAEVEY
210 220 230 240 250
HDVTSPSAFY GEQVVDGKGV LDENTYMVVW TTTPWTIPAS EGITIDATFD
260 270 280 290 300
YAVVQHDDDE RKYVLAADLV NADAELFGWN DVKIVKTVKG AELENVLCQH
310 320 330 340 350
PFYPERKLVT MLGDFVTTDA GTGLVHTAPG FGEDDFNIGV KYGLDVYVPV
360 370 380 390 400
DDKGYMTEDT GEDFAGLFYE DANEVSLKKL EEAGVLLKQM DYEHSYPFDW
410 420 430 440 450
RTKKPIIFRA TPQWFASVDK IRDQILGAIN EVQFFPDWGQ KRLYNMIRDR
460 470 480 490 500
GDWVISRQRV WGVPLPIFYG EDGEAIMTKE TIEHVAKLVE EHGSNIWFQR
510 520 530 540 550
EAKDLLPEGF TSEHSPNGKF TKETDIMDVW FDSGSSHQGV CAERDYLTYP
560 570 580 590 600
ADLYLEGSDQ YRGWFNSSLI TSVAYSGHAP YKQILSQGFT LDGKGRKMSK
610 620 630 640 650
SLGNTIVPSE VIDKMGAEII RLWVLSVDTS ADVRVSMGSF QQIAESYRKF
660 670 680 690 700
RNTVRFLLAN TTDFDPAKNA VAFDEMESID KYMTVLVNKF TKEILDAYAN
710 720 730 740 750
YEFMEIYKKL INFITTDLSA FYMDVAKDVV YIEAPDSKKR RSMQTVLYDV
760 770 780 790 800
VVRLTKLMTP ILPHTTEEIW KYLKEDEEYA QLSEMPEVKH FNNEEKLVDL
810 820 830 840 850
WNRFMNLRSG VFKALEEARN EKLIGKSFEA HVDLYVSNGV QADLDALNAN
860 870 880 890 900
VRQALIVSAL DVHPLSEAPE NALKFNDEYA VVVEHAEGEV CPRCRMIKTD
910 920 930
IGSDADLPTL CASCAEIVRE NYPEALTEGL E
Length:931
Mass (Da):106,202
Last modified:May 2, 2006 - v1
Checksum:i5DC79435C3CACD89
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000233 Genomic DNA. Translation: ABD99850.1.
RefSeqiYP_535933.1. NC_007929.1.

Genome annotation databases

EnsemblBacteriaiABD99850; ABD99850; LSL_1042.
GeneIDi3976743.
KEGGilsl:LSL_1042.
PATRICi22278987. VBILacSal43464_1115.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000233 Genomic DNA. Translation: ABD99850.1 .
RefSeqi YP_535933.1. NC_007929.1.

3D structure databases

ProteinModelPortali Q1WTA9.
SMRi Q1WTA9. Positions 1-920.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 362948.LSL_1042.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD99850 ; ABD99850 ; LSL_1042 .
GeneIDi 3976743.
KEGGi lsl:LSL_1042.
PATRICi 22278987. VBILacSal43464_1115.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci LSAL362948:GJDJ-1120-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UCC118.

Entry informationi

Entry nameiSYI_LACS1
AccessioniPrimary (citable) accession number: Q1WTA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 2, 2006
Last modified: October 29, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3